CAS 117548-22-8: 5(6)-Carboxyfluorescein, Nhs Ester
Description:5(6)-Carboxyfluorescein, N-hydroxysuccinimide (NHS) ester is a fluorescent dye commonly used in bioconjugation and labeling applications. This compound features a carboxyfluorescein moiety, which provides strong fluorescence properties, making it suitable for various imaging techniques. The NHS ester functional group allows for the formation of stable amide bonds with primary amines, facilitating the conjugation of the dye to proteins, peptides, or other biomolecules. The compound is typically soluble in organic solvents and exhibits a high degree of photostability, which is advantageous for long-term experiments. Its fluorescence emission is generally in the green region of the spectrum, making it compatible with many fluorescence detection systems. Additionally, 5(6)-Carboxyfluorescein, NHS ester is often utilized in assays and research involving cellular uptake studies, tracking biomolecules, and in the development of fluorescent probes. Care should be taken when handling this compound, as it can be sensitive to light and moisture, which may affect its stability and performance in experimental settings.
Formula:C50H30N2O18
InChI:InChI=1/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)
- Synonyms:
- 5(6)-Carboxyfluorescein N-Hydroxysuccinime Ester
- 5(6)-Carboxyfluorescein N-succinimidyl ester
- Fluos
- 5-(And-6)-Carboxyfluorescein, Succinimidyl Ester
- 5-(And-6)-Carboxy Snafl(R)-1, Succinimidyl Ester
- 5-(And-6)-Carboxy Snafl(R)-2, Succinimidyl Ester
- 5(6)-carboxyfluorescein N-succinimidyl ester,mixt.of isom.
- 5(6)-Carboxyfluorescein N-Hydroxysuccinimide Ester
- 5,6-Carboxyfluorescein Se
- 5(6)-Fam Se
- See more synonyms
- 3-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid-4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid (1:1)
