CAS 117548-22-8

5(6)-Carboxyfluorescein, Nhs Ester

Description:

5(6)-Carboxyfluorescein, N-hydroxysuccinimide (NHS) ester is a fluorescent dye commonly used in bioconjugation and labeling applications. This compound features a carboxyfluorescein moiety, which provides strong fluorescence properties, making it suitable for various imaging techniques. The NHS ester functional group allows for the formation of stable amide bonds with primary amines, facilitating the conjugation of the dye to proteins, peptides, or other biomolecules. The compound is typically soluble in organic solvents and exhibits a high degree of photostability, which is advantageous for long-term experiments. Its fluorescence emission is generally in the green region of the spectrum, making it compatible with many fluorescence detection systems. Additionally, 5(6)-Carboxyfluorescein, NHS ester is often utilized in assays and research involving cellular uptake studies, tracking biomolecules, and in the development of fluorescent probes. Care should be taken when handling this compound, as it can be sensitive to light and moisture, which may affect its stability and performance in experimental settings.

Formula: C₅₀H₃₀N₂O₁₈

InChI: InChI=1/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

SMILES: c1cc(c(cc1C(=O)ON1C(=O)CCC1=O)c1c2ccc(cc2oc2cc(=O)ccc12)O)C(=O)O.c1cc(c(c(c1)C(=O)ON1C(=O)CCC1=O)c1c2ccc(cc2oc2cc(=O)ccc12)O)C(=O)O

Synonyms:

• 5(6)-Carboxyfluorescein N-Hydroxysuccinime Ester
• 5(6)-Carboxyfluorescein N-succinimidyl ester
• Fluos
• 5-(And-6)-Carboxyfluorescein, Succinimidyl Ester
• 5-(And-6)-Carboxy Snafl(R)-1, Succinimidyl Ester
• 5-(And-6)-Carboxy Snafl(R)-2, Succinimidyl Ester
• 5(6)-carboxyfluorescein N-succinimidyl ester,mixt.of isom.
• 5(6)-Carboxyfluorescein N-Hydroxysuccinimide Ester
• 5,6-Carboxyfluorescein Se
• 5(6)-Fam Se
• 3-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid-4-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid (1:1)

5(6)-FAM SE

CAS: 117548-22-8

5(6)-FAM SE

Formula: C₅₀H₃₀N₂O₁₈

Purity: ≥90%

Molecular weight: 946.786

Ref: 54-BUP14498

5(6)-Carboxyfluorescein N-succinimidyl ester

CAS: 117548-22-8

Formula: C₂₆H₁₉NO₉

Purity: 95%

Color and Shape: Solid

Molecular weight: 489.4304

Ref: IN-DA008R05

5(6)-Carboxyfluorescein N-hydroxy succinimide ester

CAS: 117548-22-8

5(6)-Carboxyfluorescein N-hydroxy succinimide ester

Formula: C₂₅H₁₅NO₉

Purity: 98%

Color and Shape: dark yellow orange powder

Molecular weight: 473.39g/mol

Ref: 54-BIC1074

5(6)-Carboxyfluorescein N-Hydrosuccinimide ester

CAS: 117548-22-8

Formula: C₂₅H₁₅NO₉

Molecular weight: 473.39

Ref: 4Z-F-139001

5(6)-FAM SE

CAS: 117548-22-8

5(6)-FAM SE is a green dye that reacts with amines, often used for protein labeling.

Formula: C₅₀H₃₀N₂O₁₈

Purity: 95.04% - 99.77%

Color and Shape: Solid

Molecular weight: 946.786

Ref: TM-T18895

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

CAS: 117548-22-8

Formula: C₂₅H₁₅NO₉

Purity: ≥ 98.0%

Color and Shape: Yellow to orange powder

Molecular weight: 473.39

Ref: 7W-GT9499

5(6)-Carboxyfluorescein succinimidyl ester, mixture of isomers

CAS: 117548-22-8

(5)6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies, it is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions. 5(6)-Carboxyfluorescein with succinimidyl ester-activated carboxylic acids react with primary amines.

Formula: C₂₅H₁₅NO₉

Purity: Min. 95 Area-%

Color and Shape: Powder

Molecular weight: 473.39 g/mol

Ref: 3D-FC45649

5(6)-FAM SE

CAS: 117548-22-8

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

Formula: C₂₅H₁₅NO₉

Purity: 95% (isomer mixture)

Molecular weight: 473.39

Ref: IN-DG130603