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CAS 1185-53-1

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Tris(hydroxymethyl)aminomethane hydrochloride

Description:
Tris(hydroxymethyl)aminomethane hydrochloride, commonly known as Tris hydrochloride, is a white crystalline powder that is highly soluble in water. It is a buffering agent widely used in biochemistry and molecular biology to maintain a stable pH in various biological and chemical reactions. The compound features three hydroxymethyl groups attached to an amino group, which contributes to its buffering capacity, particularly in the physiological pH range. Tris hydrochloride is often utilized in the preparation of buffers for electrophoresis, cell culture, and enzyme assays. Its pKa value is around 8.1, making it effective for buffering solutions near neutral pH. Additionally, Tris hydrochloride is relatively non-toxic and has low reactivity, which makes it suitable for use in sensitive biological applications. However, it is important to note that Tris can interfere with certain assays, particularly those involving phenolic compounds. Proper handling and storage conditions should be observed to maintain its stability and effectiveness.
Formula:C4H11NO3·ClH
InChI:InChI=1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key:InChIKey=QKNYBSVHEMOAJP-UHFFFAOYSA-N
SMILES:C(CO)(CO)(CO)N.Cl
Synonyms:
  • 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-, Chloride
  • 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1)
  • 1,3-Propanediol,2-amino-2-(hydroxymethyl)-,hydrochloride
  • 2-Amino-2-(Hidroximetil)Propano-1,3-Diol, Clorhidrato
  • 2-Amino-2-(Hydroxymethyl)-1,3-Propanediol, Hydrochloride
  • 2-Amino-2-(Hydroxymethyl)Propane-1,3-Diol Hydrochloride
  • 2-Amino-2-(Hydroxymethyl)Propane-1,3-Diol Hydrochloride (1:1)
  • 2-Amino-2-(hydroxymethyl)propan-1,3-diolhydrochlorid
  • 2-Amino-2-(hydroxymethyl)propane-1,3-diol, chlorhydrate
  • 3-Propanediol,2-Amino-2-(Hydroxymethyl)-Hydrochloride
  • See more synonyms
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