CAS 1230-27-9

P-nitrophenyl-1-thio-B-D-*galactopyranoside

Description:

P-nitrophenyl-1-thio-β-D-galactopyranoside, commonly referred to as PNPG, is a synthetic compound used primarily as a substrate in enzymatic assays, particularly for the detection of β-galactosidase activity. It features a p-nitrophenyl group, which provides a chromogenic response upon hydrolysis, making it useful for colorimetric assays. The compound is a thio-glycoside, indicating the presence of a sulfur atom in its structure, which contributes to its reactivity. Upon enzymatic cleavage by β-galactosidase, PNPG releases p-nitrophenol, a yellow compound that can be quantified spectrophotometrically. This property makes PNPG a valuable tool in molecular biology and biochemistry for studying enzyme kinetics and carbohydrate metabolism. The compound is typically soluble in organic solvents and has limited solubility in water, which is an important consideration for experimental applications. Safety data should be reviewed, as with any chemical, to ensure proper handling and disposal.

Formula: C₁₂H₁₅NO₇S

InChI: InChI=1/C12H15NO7S/c14-5-8-9(15)10(16)11(17)12(20-8)21-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8?,9-,10+,11-,12+/m1/s1

Synonyms:

• p-Nitrophenyl-1-thio-?-D-galactoside

4-Nitrophenyl 1-thio-β-D-galactopyranoside

CAS: 1230-27-9

Formula: C₁₂H₁₅NO₇S

Color and Shape: Solid

Molecular weight: 317.3150

Ref: IN-DA000JXH

4-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1230-27-9

4-Nitrophenyl b-D-thiogalactopyranoside is a specialized chromogenic substrate designed for measuring the activity of thio-β-galactosidase enzymes. Upon cleavage by thio-β-galactosidase, it releases the intensely colored 4-nitrophenolate ion, providing a straightforward, sensitive, and quantitative detection method. This substrate is easily incorporated into enzyme-coupled assays, making it a reliable and convenient choice for research and diagnostic applications. With its rapid color development and high signal-to-background ratio, 4-Nitrophenyl b-D-thiogalactopyranoside is ideal for monitoring enzyme kinetics, substrate specificity evaluation, and inhibitor screening in a range of scientific and biotechnological settings.

Formula: C₁₂H₁₅NO₇S

Purity: Min. 98 Area-%

Color and Shape: Powder

Molecular weight: 317.32 g/mol

Ref: 3D-EN04995