CAS 125328-83-8

4-METHYLUMBELLIFERYL PHOSPHATE, DILITHIUM SALT

Description:

4-Methylumbelliferyl phosphate, dilithium salt, is a chemical compound commonly used as a substrate in biochemical assays, particularly for the detection of phosphatase activity. It is characterized by its fluorescent properties, which are activated upon hydrolysis by phosphatases, resulting in the release of 4-methylumbelliferone, a fluorescent product. This compound is typically soluble in water and exhibits a high degree of stability under standard laboratory conditions. The dilithium salt form enhances its solubility and facilitates its use in various biological applications. Its fluorescence can be measured using a fluorometer, making it a valuable tool in enzymatic assays and research involving enzyme kinetics. Additionally, the compound is often utilized in studies related to signal transduction and cellular processes, where phosphatase activity plays a crucial role. Safety data sheets should be consulted for handling and storage guidelines, as with any chemical substance, to ensure proper laboratory practices.

Formula: C₁₀H₇O₆P

InChI: InChI=1/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)/p-2

InChI key: SNYWUWHQGOCFJJ-UHFFFAOYSA-L

SMILES: Cc1cc(=O)oc2cc(ccc12)OP(=O)([O-])[O-]

Synonyms:

• 4-Methyl-7-(Phosphonooxy)-2H-1-Benzopyran-2-Ondilithiumsalt
• 4-Mu-Phos Dili
• 4-Methylumbelliferylphosphate Dilithium Salt Hydrate
• 4-Methylumbelliferylphosphat Dilithiumsalz
• Mup Dili
• 4-Methylumbelliferyl Phosphate*Dilithium
• 4-Methylumberlliferylphosphatedilithiumsalt
• 4-methyl-2-oxo-2H-chromen-7-yl phosphate

4-Methylumbelliferyl phosphate, dilithium salt

CAS: 125328-83-8

4-Methylumbelliferyl phosphate, dilithium salt

Formula: C₁₀H₇O₆P·2Li·H₂O

Color and Shape: Solid

Molecular weight: 286.03002

Ref: 54-BIB6117

4-Methylumbelliferyl phosphate dilithium

CAS: 125328-83-8

4-Methylumbelliferyl phosphate dilithium is a fluorogenic substrate for alkaline phosphatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl phosphate dilithiumas a substrate for measuring the alkaline phosphatase activity is used for bone metabolism disorder studies and hypophosphatasia diagnosis.

Formula: C₁₀H₇O₆P•Li₂

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 268.01 g/mol

Ref: 3D-EM52229