CAS 128802-73-3

succinimidyl-alanyl-phenylalanyl-prolyl-phenylalanine 4-nitroanilide

Description:

Succinimidyl-alanyl-phenylalanyl-prolyl-phenylalanine 4-nitroanilide, identified by its CAS number 128802-73-3, is a synthetic peptide substrate commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound features a succinimidyl group that facilitates its conjugation to various biomolecules, enhancing its utility in labeling and detection applications. The peptide sequence includes amino acids such as alanine, phenylalanine, and proline, which contribute to its structural and functional properties. The presence of the 4-nitroanilide moiety is significant as it serves as a chromogenic group, allowing for the quantification of enzymatic activity through colorimetric assays. This compound is typically soluble in organic solvents and exhibits stability under standard laboratory conditions. Its specific interactions with enzymes, particularly proteases, make it valuable for studying enzyme kinetics and mechanisms. Overall, succinimidyl-alanyl-phenylalanyl-prolyl-phenylalanine 4-nitroanilide is an important tool in biochemical assays and research involving protein interactions and enzymatic functions.

Formula: C₃₆H₄₀N₆O₉

InChI: InChI=1/C36H40N6O9/c1-23(38-31(43)18-19-32(44)45)33(46)39-29(21-24-9-4-2-5-10-24)36(49)41(26-14-16-27(17-15-26)42(50)51)30(22-25-11-6-3-7-12-25)35(48)40-34(47)28-13-8-20-37-28/h2-7,9-12,14-17,23,28-30,37H,8,13,18-22H2,1H3,(H,38,43)(H,39,46)(H,44,45)(H,40,47,48)/t23-,28-,29-,30-/m0/s1

SMILES: C[C@@H](C(=N[C@@H](Cc1ccccc1)C(=O)N(c1ccc(cc1)N(=O)=O)[C@@H](Cc1ccccc1)C(=O)N=C([C@@H]1CCCN1)O)O)N=C(CCC(=O)O)O

Synonyms:

• Suc-ala-phe-pro-phe-pna
• Suc-apppa
• N-(3-carboxypropanoyl)-L-alanyl-L-phenylalanyl-Nalpha-(4-nitrophenyl)-N-[(2S)-pyrrolidin-2-ylcarbonyl]-L-phenylalaninamide
• Succinimidyl-alanyl-phenylalanyl-prolyl-phenylalanine 4-nitroanilide

Suc-Ala-Phe-Pro-Phe-pNA

CAS: 128802-73-3

Substrate for FK-506 binding proteins (FKBPs, also called macrophilins) and cyclophilins, which belong to the group of peptidyl prolyl cis-trans isomerases (PPIases). Suc-AFPF-pNA has been used for an uncoupled protease-free assay of PPIase activity.

Formula: C₃₆H₄₀N₆O₉

Purity: > 99%

Color and Shape: Light Yellow Powder

Molecular weight: 700.75

Ref: 01-4016001

Suc-Ala-Phe-Pro-Phe-pNA

CAS: 128802-73-3

Suc-Ala-Phe-Pro-Phe-pNA is a recombinant isomerase that has been shown to be an immunosuppressive agent. It is able to catalyze the conversion of cyclosporin A into its two inactive forms, as well as the conversion of cyclophilins A and B into their corresponding inactive forms. The enzyme has also been shown to have chaperone activity. Suc-Ala-Phe-Pro-Phe-pNA has been expressed in Escherichia coli and purified from inclusion bodies using an affinity column with immobilized recombinant cyclophilin A and B. The enzyme's activity was measured by catalysis of the conversion of pNPP, a substrate analogue, into pNPPdG, which can be detected spectrophotometrically at a wavelength of 340 nm. Suc-Ala-Phe-Pro-Phe-pNA shows exponential growth in the presence of

Formula: C₃₆H₄₀N₆O₉

Purity: Min. 95%

Molecular weight: 700.74 g/mol

Ref: 3D-FS110764