CAS 143744-88-1

DANSYL-GCVLS

Description:

DANSYL-GCVLS is a synthetic compound characterized by its unique structure, which includes a dansyl group, a fluorescent moiety commonly used in biochemistry for labeling and detection purposes. The compound is often utilized in peptide synthesis and studies involving protein interactions due to its ability to fluoresce under UV light, making it a valuable tool in various analytical techniques, including fluorescence microscopy and spectroscopy. The presence of the GCVLS sequence indicates that it is a peptide derivative, which may play a role in biological processes or serve as a model for studying peptide behavior. DANSYL-GCVLS is soluble in organic solvents, and its fluorescence properties can be influenced by the surrounding environment, such as pH and polarity. This compound is typically handled with care in laboratory settings, as with many synthetic peptides, to ensure safety and integrity during experiments. Overall, DANSYL-GCVLS is an important reagent in biochemical research, particularly in the fields of proteomics and molecular biology.

Formula: C₃₁H₄₆N₆O₉S₂

Synonyms:

• Dansyl-Gly-Cys-Val-Leu-Ser-Oh
• Dns-Gcvls
• L-Serine, N-[[5-(dimethylamino)-1-naphthalenyl]sulfonyl]glycyl-L-cysteinyl-L-valyl-L-leucyl-
• DANSYL-GCVLS

Dansyl-Gly-Cys-Val-Leu-Ser-OH

CAS: 143744-88-1

Fluorogenic substrate for farnesyl diphosphate farnesyltransferase (FTase). The pentapeptide is based on the C-terminal region of H-Ras with a dansyl group attached to the N-terminus. Due to farnesylation of the cysteine thiol group the dansyl group is placed from a polar to a non-polar molecular environment which is accompanied by an enhancement of fluorescence and a shift to a lower wavelength emission maximum of the dansyl group. Complete conversion of the product results in a decrease of the emission maximum wavelength from 565 nm to 515 nm together with a 13-fold enhancement in fluorescence intensity at 505 nm. Dansyl-GCVLS can be used for continuously monitoring FTase activity in the presence of farnesyl diphosphate (FPP) at 505 nm using an excitation wavelength of 340 nm (kcat = 0.5 s⁻¹; Km = 1.4 µM). It represents a useful tool for screening potential FTase inhibitors. The substrate is highly specific to FTase and is not recognized by geranylgeranyl transferse type I (GGTase I). Concentrations of stock solutions of Dns-GCVLS can be calculated from the extinction coefficient of the dansyl moiety (ε₃₄₀ = 4250 M⁻¹cm⁻¹ in 20 mM Tris-HCl, pH 7.5, 10 mM EDTA).

Formula: C₃₁H₄₆N₆O₉S₂

Purity: 92.2%

Color and Shape: Light Yellow

Molecular weight: 710.87

Ref: 01-4037632

Dansyl-Gly-Cys-Val-Leu-Ser-OH

CAS: 143744-88-1

Dansyl-Gly-Cys-Val-Leu-Ser-OH is an α subunit of the enzyme farnesyl diphosphate synthase. Dansyl-Gly-Cys-Val-Leu-Ser-OH is a substrate for this enzyme, which catalyzes the conversion of prenyl pyrophosphates to farnesyl pyrophosphate. The biological function of this peptide is not well understood, but it may be involved in cholesterol synthesis or cellular signaling. This peptide binds to cell membranes and has been shown to have a fluorescent property. Dansyl-Gly-Cys-Val-Leu-Ser OH can also bind to peptides and proteins, including the protein receptor for the HIV virus (CD4).

Formula: C₃₁H₄₆N₆O₉S₂

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 710.86 g/mol

Ref: 3D-FD110983