CAS 165174-57-2

N-succinyl-gly-phe-gly P-nitroanilide

Description:

N-succinyl-gly-phe-gly P-nitroanilide, with the CAS number 165174-57-2, is a synthetic peptide substrate commonly used in biochemical assays, particularly for the detection of proteolytic enzymes. This compound features a succinyl group that enhances its solubility and stability, along with a sequence of amino acids—glycine, phenylalanine, and another glycine—contributing to its specificity for certain enzymes. The P-nitroanilide moiety serves as a chromogenic indicator, allowing for the quantification of enzymatic activity through colorimetric changes upon cleavage by proteases. The compound is typically characterized by its relatively low molecular weight and the presence of functional groups that facilitate interactions with enzymes. Its applications extend to research in enzymology, drug discovery, and the development of diagnostic assays, making it a valuable tool in both academic and industrial laboratories. Proper handling and storage conditions are essential to maintain its stability and reactivity in experimental settings.

Formula: C₂₃H₂₅N₅O₈

Synonyms:

• Suc-Gly-Phe-Gly-pNA
• SUC-GFG-PNA
• Glycinamide, N-(3-carboxy-1-oxopropyl)glycyl-L-phenylalanyl-N-(4-nitrophenyl)- (9CI)
• N-SUCCINYL-GLY-PHE-GLY P-NITROANILIDE

Suc-Gly-Phe-Gly-pNA tifluoroacetic acid

CAS: 165174-57-2

Suc-Gly-Phe-Gly-pNA is a synthetic substrate for proteolytic enzymes that is used to measure the activity of these enzymes. The substrate is hydrolysed by the enzyme to produce an amino acid sequence that is then measured using high performance liquid chromatography (HPLC). Suc-Gly-Phe-Gly-pNA was found to be more sensitive than thioglycolate, which is commonly used as a substrate in protease assays. This product has been shown to have maximal activity at alkaline pH and can be used as a substitute for thioglycolate in protease assays.

Formula: C₂₃H₂₅N₅O₈•(CF₃CO₂H)x

Purity: Min. 95%

Molecular weight: 499.47 g/mol

Ref: 3D-FS110773