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CAS 19887-85-5

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O-nitrophenyl-A-D-galactopyranoside

Description:
O-nitrophenyl-α-D-galactopyranoside (ONPG) is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of galactosidase activity. It is a colorless, crystalline solid that is soluble in water and organic solvents. The compound features a nitrophenyl group, which imparts a yellow color upon hydrolysis, making it useful for spectrophotometric measurements. ONPG is a derivative of galactose and is characterized by its ability to undergo enzymatic cleavage by β-galactosidase, resulting in the release of o-nitrophenol, which can be quantitatively measured. This property makes ONPG a valuable tool in molecular biology and microbiology for studying enzyme kinetics and microbial activity. Additionally, it is important to handle ONPG with care, as it may pose health risks if ingested or inhaled, and appropriate safety measures should be taken during its use in laboratory settings.
Formula:C12H15NO8
InChI:InChI=1/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11+,12-/m0/s1
Synonyms:
  • 2-nitrophenyl beta-L-galactopyranoside
  • 2-nitrophenyl alpha-L-galactopyranoside
  • 2-nitrophenyl alpha-L-talopyranoside
  • 2-nitrophenyl beta-L-talopyranoside
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