CAS 212118-77-9

FlAsH

Description:

FlAsH, with the CAS number 212118-77-9, is a chemical compound known for its application in biological research, particularly in the field of fluorescence microscopy. It is a small, cell-permeable molecule that serves as a fluorescent probe for detecting specific proteins or cellular structures. FlAsH is characterized by its ability to bind selectively to tetracysteine-tagged proteins, allowing for visualization and tracking of these proteins within live cells. The compound exhibits strong fluorescence properties, making it useful for imaging techniques. Additionally, FlAsH is typically non-toxic to cells, which is crucial for live-cell imaging applications. Its specificity and brightness make it a valuable tool in studies involving protein localization, dynamics, and interactions in various biological systems. Overall, FlAsH is an important reagent in molecular biology and biochemistry, facilitating advancements in our understanding of cellular processes.

Formula: C₂₄H₁₈As₂O₅S₄

InChI: InChI=1S/C24H18As2O5S4/c27-17-7-5-15-21(19(17)25-32-9-10-33-25)30-22-16(6-8-18(28)20(22)26-34-11-12-35-26)24(15)14-4-2-1-3-13(14)23(29)31-24/h1-8,27-28H,9-12H2

InChI key: InChIKey=AJNUQUGWNQHQDJ-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C(=C(C(O)=CC3)[As]4SCCS4)OC=5C2=CC=C(O)C5[As]6SCCS6)C=7C1=CC=CC7

Synonyms:

• FlAsH-EDT2
• Lumio Green
• Fluorescein arsenical helix binder
• 4′,5′-Bis(1,3,2-dithiarsolan-2-yl)-3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 4′,5′-bis(1,3,2-dithiarsolan-2-yl)-3′,6′-dihydroxy-

FlAsH-EDT2

CAS: 212118-77-9

FlAsH-EDT2

Purity: 98%

Molecular weight: 664.5g/mol

Ref: 54-OR1037116

FlAsH-EDT2

Controlled Product

CAS: 212118-77-9

Applications The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin.
Not a dangerous good if item is equal to or less than 1g/ml and there is less than 100g/ml in the package
References Gaietta, G., et al.: Science, 296, 503 (2002), Hoffmann, C., et al.: Nature Methods, 2, 171 (2005), Dyachok, O., et al.: Nature, 439, 349 (2006), Gautier, A., et al.: Chem. Biol., 15, 128 (2008),

Formula: C₂₄H₁₈As₂O₅S₄

Color and Shape: White To Light Orange

Molecular weight: 664.50

Ref: TR-F335200

FlAsH-EDT2

CAS: 212118-77-9

FlAsH-EDT2 is a red fluorescent dye that binds to calcium ions. It has been shown to inhibit the growth of wild-type herpes simplex virus, while not affecting the growth of recombinant viruses with mutated sequences. FlAsH-EDT2 binds to the kinase domain of soluble guanylate cyclase and inhibits its activity, thereby inhibiting cellular proliferation in response to extracellular growth factors. This inhibition can be reversed by removing excess calcium ions from the system. The binding of FlAsH-EDT2 to calcium ions also results in changes in electrophysiological properties.

Formula: C₂₄H₁₈As₂O₅S₄

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 664.5 g/mol

Ref: 3D-FF23296

FlAsH-EDT2

CAS: 212118-77-9

Purity: 98%

Molecular weight: 664.4899902

Ref: 10-F608863

FlAsH-EDT2

CAS: 212118-77-9

FlAsH-EDT2 is a membrane-permeable fluorescent reagent that can label proteins. FlAsH-EDT2 can bind to CCXXCC motifs and nonspecific endogenous cysteine-rich proteins. FlAsH-EDT2 can only be used to label recombinant proteins that are expressed at very high levels.

Formula: C₂₄H₁₈As₂O₅S₄

Purity: 98%

Color and Shape: Solid

Molecular weight: 664.5

Ref: TM-T31797