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CAS 27565-41-9

:

Dithiothreitol

Description:
Dithiothreitol (DTT), with the CAS number 27565-41-9, is a small-molecule reducing agent commonly used in biochemistry and molecular biology. It is characterized by its ability to break disulfide bonds in proteins, thereby maintaining proteins in a reduced state and preventing oxidation. DTT is a colorless, crystalline solid that is soluble in water and has a slightly sweet odor. Its chemical structure features two thiol (-SH) groups, which are responsible for its reducing properties. DTT is often employed in protein purification, enzyme assays, and the stabilization of proteins during storage and analysis. It is important to handle DTT with care, as it can be toxic in high concentrations and may cause irritation upon contact with skin or eyes. Additionally, DTT is sensitive to air and should be stored in a cool, dry place, preferably under inert gas conditions to prevent oxidation. Overall, DTT is a vital reagent in various biochemical applications due to its effectiveness in maintaining the reduced state of biomolecules.
Formula:C4H10O2S2
InChI:InChI=1/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/s2
InChI key:InChIKey=VHJLVAABSRFDPM-SEFKMRKONA-N
SMILES:[C@H]([C@@H](CS)O)(CS)O
Synonyms:
  • (2S,3S)-1,4-disulfanylbutane-2,3-diol
  • (R*,R*)-1,4-Dimercapto-2,3-butanediol
  • (R,R)-Dithiothreitol
  • (±)-1,4-Dimercapto-2,3-butanediol
  • (±)-Dithiothreitol
  • 1,4-Dithio-<span class="text-smallcaps">DL</span>-threitol
  • 1,4-Dithio-DL-threitol
  • 1,4-Dithio-Dl-Threitol Sol.
  • 1,4-Dithio-l-threitol
  • 1,4-Dithiothreitol
  • See more synonyms
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