CAS 28718-90-3

4′,6-Diamidino-2-phenylindole dihydrochloride

Description:

4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) is a fluorescent stain that binds strongly to A-T rich regions in DNA, making it a valuable tool in molecular biology and microscopy. It is commonly used for staining cell nuclei in fluorescence microscopy, allowing researchers to visualize and quantify cellular structures. DAPI emits blue fluorescence when excited by ultraviolet light, which facilitates the identification of cells and their nuclei in various biological samples. The compound is soluble in water and exhibits a high affinity for double-stranded DNA, which enhances its utility in applications such as cell cycle analysis and apoptosis studies. Additionally, DAPI is known for its low toxicity, making it suitable for live-cell imaging under specific conditions. However, it is important to handle DAPI with care, as it can be a potential mutagen. Overall, DAPI is a widely used reagent in the fields of cell biology, histology, and molecular genetics due to its effective staining properties and ease of use.

Formula: C₁₆H₁₅N₅·2ClH

InChI: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key: InChIKey=FPNZBYLXNYPRLR-UHFFFAOYSA-N

SMILES: C(=N)(N)C=1C=C2C(C=C(N2)C3=CC=C(C(=N)N)C=C3)=CC1.Cl

Synonyms:

• 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride
• 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)
• 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
• 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
• 2-Phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate)
• 4′,6-Diamidino-2-phenylindole dihydrochloride
• 6-Amidino-2-(4-amidinophenyl)indole dihydrochloride
• Ccris 8836
• FxCycle Violet
• Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dihydrochloride

DAPI 2HCl [for Biochemical Research]

CAS: 28718-90-3

Formula: C₁₆H₁₅N₅·2HCl

Purity: >90.0%(HPLC)

Color and Shape: Light yellow to Yellow to Orange powder to crystal

Molecular weight: 350.25

Ref: 3B-A2412

DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

CAS: 28718-90-3

Formula: C₁₆H₁₅N₅·2HCl

Color and Shape: Light yellow to Yellow clear liquid

Molecular weight: 350.25

Ref: 3B-D5888

1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)

CAS: 28718-90-3

Formula: C₁₆H₁₇Cl₂N₅

Purity: 97%

Color and Shape: Solid

Molecular weight: 350.2457

Ref: IN-DA002W8S

DAPI Dihydrochloride

CAS: 28718-90-3

DAPI Dihydrochloride

Purity: ≥98%

Molecular weight: 350.24g/mol

Ref: 54-BUP10615

4',6-Diamidino-2-phenylindole dihydrochloride

CAS: 28718-90-3

4',6-Diamidino-2-phenylindole dihydrochloride

Formula: C₁₆H₁₅N₅·2ClH

Purity: 98%

Color and Shape: yellow solid

Molecular weight: 350.24568g/mol

Ref: 54-BID0433

4'',6-Diamidino-2-phenylindole dihydrochloride

CAS: 28718-90-3

Formula: C₁₆H₁₅N₅·2HCl

Purity: ≥ 95.0%

Color and Shape: Yellow powder

Molecular weight: 350.3

Ref: 7W-GT2414

DAPI Dihydrochloride

CAS: 28718-90-3

DAPI Dihydrochloride (FxCycle Violet) is a cell-permeable fluorescent probe, to bound in groves of A-T-rich DNA sequences. High-Quality, Low-Cost!

Formula: C₁₆H₁₇Cl₂N₅

Purity: 98.83% - 99.94%

Color and Shape: Yellow Solid

Molecular weight: 350.24

Ref: TM-T19827

2-(4-CARBAMIMIDOYLPHENYL)-1H-INDOLE-6-CARBOXIMIDAMIDE 2HCL

CAS: 28718-90-3

Purity: 97.0%

Color and Shape: Solid

Molecular weight: 350.25

Ref: 10-F053590

4’,6-Diamidino-2-phenylindole Dihydrochloride

Controlled Product

CAS: 28718-90-3

Stability Hygroscopic
Applications A cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
References Jpeeesen, C, and Nielsen, P.E.: Eur. J. Biochem., 182, 437 (1989), Li,J., et al.: J. Exp. Biol., 200, 1499 (1997), Zaitsev, E.N., and Kowalczykowsi, S.C.: Nucl. Acids Res., 26(2), 650 (1998), Germ, M., et al.: Biochem. Biophys. Res. Commun., 261, 452 (1999),

Formula: C₁₆H₁₅N₅·2ClH

Color and Shape: Yellow Powder

Molecular weight: 350.25

Ref: TR-D416050

4',6-Diamidino-2-phenylindole 2HCl

CAS: 28718-90-3

4',6-Diamidino-2-phenylindole 2HCl (DAB) is a chemical compound used as a histological stain to detect and identify different types of cancer cells. It has been shown to have cytotoxic activity against cancer cells in vitro, but not normal cells. DAB inhibits the growth of cancer cells by disrupting the mitochondrial membrane potential, leading to the release of cytochrome c and apoptosis. The use of this substance has shown that it is not toxic to maternal blood or embryonic tissues. DAB binds to nuclear DNA and polymerase chain reaction products, providing an accurate way to measure cancer cell proliferation.

Formula: C₁₆H₁₇Cl₂N₅

Purity: Min. 97.5 Area-%

Color and Shape: Yellow Powder

Molecular weight: 350.25 g/mol

Ref: 3D-FD21444

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) for molecular biology, 95%

CAS: 28718-90-3

Formula: C₁₆H₁₅N₅·2HCl

Purity: min. 95%

Color and Shape: Light yellow to Yellow to Orange, Powder, Clear, Yellow

Molecular weight: 350.25

Ref: SR-18668

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) Solution (1mg/ml in H2O)

CAS: 28718-90-3

Formula: ‎C16H15N5.2HCl

Color and Shape: Clear, slight pale yellow, Liquid

Molecular weight: 350.25

Ref: SR-80145

DAPI Dihydrochloride (4,6-Diamidino-2-Phenylindole Dihydrochloride) for tissue culture, 95%

CAS: 28718-90-3

Formula: C₁₆H₁₅N₅·2HCl

Purity: min. 95%

Color and Shape: Light yellow to Yellow to Orange, Powder, Clear, Yellow

Molecular weight: 350.25

Ref: SR-28488