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CAS 28718-90-3

:

4′,6-Diamidino-2-phenylindole dihydrochloride

Description:
4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) is a fluorescent stain that binds strongly to A-T rich regions in DNA, making it a valuable tool in molecular biology and microscopy. It is commonly used for staining cell nuclei in fluorescence microscopy, allowing researchers to visualize and quantify cellular structures. DAPI emits blue fluorescence when excited by ultraviolet light, which facilitates the identification of cells and their nuclei in various biological samples. The compound is soluble in water and exhibits a high affinity for double-stranded DNA, which enhances its utility in applications such as cell cycle analysis and apoptosis studies. Additionally, DAPI is known for its low toxicity, making it suitable for live-cell imaging under specific conditions. However, it is important to handle DAPI with care, as it can be a potential mutagen. Overall, DAPI is a widely used reagent in the fields of cell biology, histology, and molecular genetics due to its effective staining properties and ease of use.
Formula:C16H15N5·2ClH
InChI:InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key:InChIKey=FPNZBYLXNYPRLR-UHFFFAOYSA-N
SMILES:C(=N)(N)C=1C=C2C(C=C(N2)C3=CC=C(C(=N)N)C=C3)=CC1.Cl
Synonyms:
  • 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, dihydrochloride
  • 1H-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)phenyl]-, hydrochloride (1:2)
  • 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
  • 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
  • 2-Phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate)
  • 4′,6-Diamidino-2-phenylindole dihydrochloride
  • 6-Amidino-2-(4-amidinophenyl)indole dihydrochloride
  • Ccris 8836
  • FxCycle Violet
  • Indole-6-carboxamidine, 2-(p-amidinophenyl)-, dihydrochloride
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