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CAS 3150-24-1

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p-Nitrophenyl β-D-galactopyranoside

Description:
p-Nitrophenyl β-D-galactopyranoside (CAS 3150-24-1) is a synthetic compound commonly used as a substrate in enzymatic assays, particularly for the detection of β-galactosidase activity. It is a glycoside, consisting of a galactopyranoside moiety linked to a p-nitrophenyl group. This compound is typically a white to pale yellow crystalline solid that is soluble in water and organic solvents like methanol and ethanol. Upon hydrolysis by β-galactosidase, it releases p-nitrophenol, which can be quantified spectrophotometrically due to its distinct yellow color, making it a valuable tool in biochemical research. The compound is stable under normal laboratory conditions but should be stored in a cool, dry place, protected from light to prevent degradation. Its use in various assays highlights its importance in molecular biology and biochemistry, particularly in studies involving lactose metabolism and enzyme kinetics.
Formula:C12H15NO8
InChI:InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChI key:InChIKey=IFBHRQDFSNCLOZ-YBXAARCKSA-N
SMILES:O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)C2=CC=C(N(=O)=O)C=C2
Synonyms:
  • 4-Nitrophenyl galactoside
  • 4-Nitrophenyl-beta-D-galactopyranoside
  • Galactopyranoside, p-nitrophenyl, β-<span class="text-smallcaps">D</span>-
  • NSC 89287
  • p-Nitrophenol galactoside
  • p-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
  • p-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactoside
  • p-Nitrophenyl-β-D-Galactopyranoside
  • p-Nitrophenyl-β-galactopyranoside
  • β-<span class="text-smallcaps">D</span>-Galactopyranoside, 4-nitrophenyl
  • See more synonyms
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