CAS 3326-32-7: Fluorescein 5-isothiocyanate

Description: Fluorescein 5-isothiocyanate (FITC) is a fluorescent dye commonly used in biological research and immunology for labeling proteins, antibodies, and nucleic acids. It is characterized by its bright green fluorescence when excited by ultraviolet or blue light, making it an excellent marker for fluorescence microscopy and flow cytometry. The compound features a fluorescein backbone, which contributes to its strong fluorescence properties, and an isothiocyanate functional group that facilitates covalent bonding to amino groups in proteins. FITC is soluble in organic solvents and water, allowing for versatile applications in various experimental conditions. Its reactivity with primary amines enables the formation of stable conjugates, which are crucial for tracking biomolecules in live cells or tissues. However, it is important to handle FITC with care, as it can be sensitive to pH changes and light exposure, which may affect its fluorescence intensity. Overall, FITC is a valuable tool in the field of biochemistry and molecular biology for visualizing and studying cellular processes.

Formula: C₂₁H₁₁NO₅S

InChI: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key: InChIKey=MHMNJMPURVTYEJ-UHFFFAOYSA-N

SMILES: O=C1OC2(C3=CC=C(O)C=C3OC4=CC(O)=CC=C42)C5=CC=C(N=C=S)C=C15

Synonyms:

• 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid
• 3′,6′-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
• 3′,6′-Dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one
• 4-Isothiocyanatofluorescein
• 5-Fitc
• 5-Isothiocyanato fluorescein
• 5-Isothiocyanatofluorescein
• Fitc 'Isomer I'
• Fitc Isomer
• Fitc isomer 1
• Fitc-Celite(R)
• Fluorescein 5-isothiocyanate
• Fluorescein Isomer I Isothiocyanate
• Fluorescein Isothiocyante
• Fluorescein isothiocyanate, isomer 1
• Fluorescein, 5-isothiocyanato-
• Fluoroscein-5-isothiocyanate
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-
• Fluorescein isothiocyanate isomer I

Fluorescein 5-Isothiocyanate (isomer I)

CAS: 3326-32-7

• Formula: C₂₁H₁₁NO₅S
• Purity: >97.0%(T)(HPLC)
• Color and Shape: White to Yellow to Orange powder to crystal
• Molecular weight: 389.38

Fluorescein isothiocyanate, isomer 1, 95%

CAS: 3326-32-7

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of …

• Formula: C₂₁H₁₁NO₅S
• Purity: 95%
• Color and Shape: Powder, Orange to brown
• Molecular weight: 389.38

FITC

CAS: 3326-32-7

FITC (Fluorescein 5-isothiocyanate) is a derivative of fluorescein for the labeling of amines.

• Formula: C₂₁H₁₁NO₅S
• Purity: 100% - 96.02%
• Color and Shape: Powder
• Molecular weight: 389.38

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-

CAS: 3326-32-7

• Formula: C₂₁H₁₁NO₅S
• Purity: 95%
• Color and Shape: Solid
• Molecular weight: 389.3807

Fluorescein Isothiocyanate Isomer I, 90%, Pure

CAS: 3326-32-7

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. …

• Formula: C₂₁H₁₁NO₅S
• Purity: 90%
• Color and Shape: Orange, Crystalline powder
• Molecular weight: 389.38

Fluorescein isothiocyanate isomer I

CAS: 3326-32-7

• Purity: 95.0%
• Color and Shape: Solid, Crystalline Powder
• Molecular weight: 389.380004882813

FITC I

CAS: 3326-32-7

• Formula: C₂₁H₁₁NO₅S
• Purity: By hplc: 96% by area (Typical Value in Batch COA)
• Color and Shape: orange powder
• Molecular weight: 389.38g/mol

Fluorescein isothiocyanate isomer I, 97.5%

CAS: 3326-32-7

• Formula: C₂₁H₁₁NO₅S
• Purity: ≥ 97.5%
• Color and Shape: Orange crystalline powder
• Molecular weight: 389.39

Fluorescein 5-Isothiocyanate, Isomer 1 (~90%)

CAS: 3326-32-7

Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification …

• Formula: C₂₁H₁₁NO₅S
• Purity: ~90%
• Color and Shape: Neat
• Molecular weight: 389.38

Fluorescein isothiocyanate Isomer I

CAS: 3326-32-7

Fluorescent reagent for labeling amines

• Formula: C₂₁H₁₁NO₅S
• Purity: Min. 90 Area-%
• Color and Shape: Orange Powder
• Molecular weight: 389.38 g/mol

Fluorescein isothiocyanate Isomer I

CAS: 3326-32-7

Fluorescent labeling reagent for proteins. FITC-conjugated antibodies are commonly used in immunohistochemistry for immunofluorescent staining. …

• Formula: C₂₁H₁₁NO₅S
• Purity: Min. 90.0 Area-%
• Molecular weight: 389.39 g/mol

Fluorescein Isothiocyanate Isomer I (FITC) extrapure, 95%

CAS: 3326-32-7

• Formula: C₂₁H₁₁NO₅S
• Purity: min. 95%
• Color and Shape: Yellow to Orange to Dark Orange Red, Powder
• Molecular weight: 389.39

Fluorescein 5-Isothiocyanate, Isomer 1, 95%

CAS: 3326-32-7

Stability Moisture Sensitive Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique …

• Formula: C₂₁H₁₁NO₅S
• Purity: 95%
• Color and Shape: Dark Orange
• Molecular weight: 389.38

5-Fluorescein isothiocyanate isomer I - 95%

CAS: 3326-32-7

5-Fluorescein isothiocyanate isomer I (5FITC) is a fluorescent probe that has been used to study …

• Formula: C₂₁H₁₁NO₅S
• Purity: Min. 95%
• Molecular weight: 389.38 g/mol