CAS 3326-32-7

Fluorescein 5-isothiocyanate

Description:

Fluorescein 5-isothiocyanate (FITC) is a fluorescent dye commonly used in biological research and immunology for labeling proteins, antibodies, and nucleic acids. It is characterized by its bright green fluorescence when excited by ultraviolet or blue light, making it an excellent marker for fluorescence microscopy and flow cytometry. The compound features a fluorescein backbone, which contributes to its strong fluorescence properties, and an isothiocyanate functional group that facilitates covalent bonding to amino groups in proteins. FITC is soluble in organic solvents and water, allowing for versatile applications in various experimental conditions. Its reactivity with primary amines enables the formation of stable conjugates, which are crucial for tracking biomolecules in live cells or tissues. However, it is important to handle FITC with care, as it can be sensitive to pH changes and light exposure, which may affect its fluorescence intensity. Overall, FITC is a valuable tool in the field of biochemistry and molecular biology for visualizing and studying cellular processes.

Formula: C₂₁H₁₁NO₅S

InChI: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key: InChIKey=MHMNJMPURVTYEJ-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC(N=C=S)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Synonyms:

• 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid
• 3′,6′-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
• 3′,6′-Dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one
• 4-Isothiocyanatofluorescein
• 5-Fitc
• 5-Isothiocyanato fluorescein
• 5-Isothiocyanatofluorescein
• Fitc 'Isomer I'
• Fitc Isomer
• Fitc isomer 1
• Fitc-Celite(R)
• Fluorescein 5-isothiocyanate
• Fluorescein Isomer I Isothiocyanate
• Fluorescein Isothiocyante
• Fluorescein isothiocyanate, isomer 1
• Fluorescein, 5-isothiocyanato-
• Fluoroscein-5-isothiocyanate
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-isothiocyanato-

Fluorescein 5-Isothiocyanate (isomer I)

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Purity: >97.0%(T)(HPLC)

Color and Shape: White to Yellow to Orange powder to crystal

Molecular weight: 389.38

Ref: 3B-F0026

Fluorescein isothiocyanate, isomer 1, 95%

CAS: 3326-32-7

Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen. This Th

Formula: C₂₁H₁₁NO₅S

Purity: 95%

Color and Shape: Powder, Orange to brown

Molecular weight: 389.38

Ref: 02-L09319

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Purity: 95%

Color and Shape: Solid

Molecular weight: 389.3807

Ref: IN-DA000E31

FITC

CAS: 3326-32-7

FITC is a green fluorescein derivative. FITC can be used as a fluorescent probe to detect cell viability, cell apoptosis, etc. High-Quality, Low-Cost!

Formula: C₂₁H₁₁NO₅S

Purity: 95.27% - >99.99%

Color and Shape: Powder

Molecular weight: 389.38

Ref: TM-T15284

FITC

CAS: 3326-32-7

FITC

Purity: ≥95%

Molecular weight: 389.38g/mol

Ref: 54-BUP16918

Fluorescein isothiocyanate isomer I

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Purity: 95.0%

Color and Shape: Solid, Crystalline Powder

Molecular weight: 389.38

Ref: 10-F471600

Fluorescein isothiocyanate isomer I, 97.5%

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Purity: ≥ 97.5%

Color and Shape: Orange crystalline powder

Molecular weight: 389.39

Ref: 7W-GT3648

FITC I

CAS: 3326-32-7

FITC I

Formula: C₂₁H₁₁NO₅S

Purity: By hplc: 96% by area (Typical Value in Batch COA)

Color and Shape: orange powder

Molecular weight: 389.38g/mol

Ref: 54-BIF4014

Fluorescein isothiocyanate Isomer I

CAS: 3326-32-7

Fluorescent labeling reagent for proteins. FITC-conjugated antibodies are commonly used in immunohistochemistry for immunofluorescent staining. Fluorescein isothiocyanate (FITC) is a yellow-green colored low molecular weight dye which couples to proteins via reaction with primary amine groups at high pH. FITC is excitable at 488nm, close to its absorption maximum at 494nm, and produces maximum fluorescence emission around 520nm. It is commonly conjugated with antibodies for use in indirect immunofluorescence.

Formula: C₂₁H₁₁NO₅S

Purity: Min. 90.0 Area-%

Molecular weight: 389.39 g/mol

Ref: 3D-F-3000

Fluorescein Isothiocyanate Isomer I (FITC) extrapure, 95%

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Purity: min. 95%

Color and Shape: Yellow to Orange to Dark Orange Red, Powder

Molecular weight: 389.39

Ref: SR-73698

Fluorescein 5-Isothiocyanate, Isomer 1 (~90%)

Controlled Product

CAS: 3326-32-7

Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.
References Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958), Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1972),

Formula: C₂₁H₁₁NO₅S

Purity: ~90%

Color and Shape: Neat

Molecular weight: 389.38

Ref: TR-F485720

Fluorescein 5-Isothiocyanate, Isomer 1, 95%

Controlled Product

CAS: 3326-32-7

Stability Moisture Sensitive
Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. Dyes and metabolites.
References Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958), Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1972),

Formula: C₂₁H₁₁NO₅S

Purity: 95%

Color and Shape: Dark Orange

Molecular weight: 389.38

Ref: TR-F485700

Fluorescein 5-Isothiocyanate, Isomer 1

CAS: 3326-32-7

Formula: C₂₁H₁₁NO₅S

Molecular weight: 389.39

Ref: ST-EA-CP-F116033