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CAS 3326-32-7

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Fluorescein 5-isothiocyanate

Description:
Fluorescein 5-isothiocyanate (FITC) is a fluorescent dye commonly used in biological research and immunology for labeling proteins, antibodies, and nucleic acids. It is characterized by its bright green fluorescence when excited by ultraviolet or blue light, making it an excellent marker for fluorescence microscopy and flow cytometry. The compound features a fluorescein backbone, which contributes to its strong fluorescence properties, and an isothiocyanate functional group that facilitates covalent bonding to amino groups in proteins. FITC is soluble in organic solvents and water, allowing for versatile applications in various experimental conditions. Its reactivity with primary amines enables the formation of stable conjugates, which are crucial for tracking biomolecules in live cells or tissues. However, it is important to handle FITC with care, as it can be sensitive to pH changes and light exposure, which may affect its fluorescence intensity. Overall, FITC is a valuable tool in the field of biochemistry and molecular biology for visualizing and studying cellular processes.
Formula:C21H11NO5S
InChI:InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key:InChIKey=MHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILES:O=C1OC2(C=3C1=CC(N=C=S)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4
Synonyms:
  • 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid
  • 3′,6′-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
  • 3′,6′-Dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one
  • 4-Isothiocyanatofluorescein
  • 5-Fitc
  • 5-Isothiocyanato fluorescein
  • 5-Isothiocyanatofluorescein
  • Fitc 'Isomer I'
  • Fitc Isomer
  • Fitc isomer 1
  • See more synonyms
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Found 16 products.
  • Fluorescein 5-Isothiocyanate (isomer I)

    CAS:
    Formula:C21H11NO5S
    Purity:>97.0%(T)(HPLC)
    Color and Shape:White to Yellow to Orange powder to crystal
    Molecular weight:389.38

    Ref: 3B-F0026

    100mg
    54.00€
    1g
    352.00€
  • Fluorescein isothiocyanate, isomer 1, 95%

    CAS:
    Fluorescein isothiocyanate is used as fluorescent labeling reagent for proteins and for rapid identification of pathogens in fluorescent antibody technique. It is also used as a reagent for microsequencing of proteins and peptides (HPLC). On celite it is used for the labeling of fibrinogen. This Th
    Formula:C21H11NO5S
    Purity:95%
    Color and Shape:Powder, Orange to brown
    Molecular weight:389.38

    Ref: 02-L09319

    50mg
    32.00€
    250mg
    102.00€
    1g
    276.00€
  • FITC

    CAS:
    3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
    Formula:C21H11NO5S
    Purity:95%
    Molecular weight:389.38

    Ref: IN-DG1290

    100mg
    35.00€
    250mg
    44.00€
    1g
    86.00€
    5g
    341.00€
    25g
    1,161.00€
  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-

    CAS:
    Formula:C21H11NO5S
    Purity:95%
    Color and Shape:Solid
    Molecular weight:389.3807

    Ref: IN-DA000E31

    25g
    To inquire
    50mg
    28.00€
    100mg
    28.00€
    250mg
    40.00€
    1g
    84.00€
    5g
    228.00€
    100g
    3,582.00€
    250g
    7,618.00€
  • FITC I

    CAS:
    FITC I
    Formula:C21H11NO5S
    Purity:By hplc: 96% by area (Typical Value in Batch COA)
    Color and Shape:Solid
    Molecular weight:389.38074

    Ref: 54-BIF4014

    100mg
    108.00€
    250mg
    186.00€
    1g
    400.00€
    2g
    758.00€
    5g
    1,303.00€
  • FITC

    CAS:
    FITC is a green fluorescein derivative. FITC can be used as a fluorescent probe to detect cell viability, cell apoptosis, etc. High-Quality, Low-Cost!
    Formula:C21H11NO5S
    Purity:95.27% - >99.99%
    Color and Shape:Powder
    Molecular weight:389.38

    Ref: TM-T15284

    50mg
    34.00€
    100mg
    47.00€
    1mL*10mM (DMSO)
    52.00€
  • FITC

    CAS:
    FITC
    Formula:C21H11NO5S
    Purity:≥95%
    Molecular weight:389.38

    Ref: 54-BUP16918

    50mg
    53.00€
    100mg
    66.00€
  • 5-FITC (isomer I) (1mg×5)

    CAS:
    Formula:C21H11NO5S
    Color and Shape:White to Yellow to Orange powder to crystal
    Molecular weight:389.38

    Ref: 3B-F1285

    1set
    89.00€
  • Fluorescein isothiocyanate isomer I

    CAS:
    Formula:C21H11NO5S
    Purity:95.0%
    Color and Shape:Solid, Crystalline Powder
    Molecular weight:389.38

    Ref: 10-F471600

    250mg
    35.00€
    1g
    85.00€
    5g
    448.00€
    25g
    1,780.00€
  • Fluorescein isothiocyanate isomer I, 97.5%

    CAS:
    Formula:C21H11NO5S
    Purity:≥ 97.5%
    Color and Shape:Orange crystalline powder
    Molecular weight:389.39

    Ref: 7W-GT3648

    100mg
    198.00€
    250mg
    243.00€
    1g
    527.00€
  • Fluorescein isothiocyanate Isomer I

    CAS:
    Fluorescent labeling reagent for proteins. FITC-conjugated antibodies are commonly used in immunohistochemistry for immunofluorescent staining. Fluorescein isothiocyanate (FITC) is a yellow-green colored low molecular weight dye which couples to proteins via reaction with primary amine groups at high pH. FITC is excitable at 488nm, close to its absorption maximum at 494nm, and produces maximum fluorescence emission around 520nm. It is commonly conjugated with antibodies for use in indirect immunofluorescence.
    Formula:C21H11NO5S
    Purity:Min. 90.0 Area-%
    Molecular weight:389.39 g/mol

    Ref: 3D-F-3000

    1g
    404.00€
    2g
    632.00€
    5g
    1,218.00€
    10g
    2,123.00€
    25g
    4,682.00€
  • FITC

    CAS:
    3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
    Formula:C21H11NO5S
    Purity:98% (HPLC)
    Molecular weight:389.38

    Ref: IN-DG01411696

    50mg
    32.00€
    100mg
    54.00€
    250mg
    87.00€
    1g
    216.00€
  • Fluorescein Isothiocyanate Isomer I (FITC) extrapure, 95%

    CAS:
    Formula:C21H11NO5S
    Purity:min. 95%
    Color and Shape:Yellow to Orange to Dark Orange Red, Powder
    Molecular weight:389.39

    Ref: SR-73698

    25mg
    28.00€
    100mg
    41.00€
    250mg
    69.00€
    1g
    207.00€
  • Fluorescein 5-Isothiocyanate, Isomer 1, 95%

    Controlled Product
    CAS:

    Stability Moisture Sensitive
    Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. Dyes and metabolites.
    References Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958), Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1972),

    Formula:C21H11NO5S
    Purity:95%
    Color and Shape:Neat
    Molecular weight:389.38

    Ref: TR-F485700

    50mg
    112.00€
    1g
    202.00€
    10g
    1,257.00€
  • Fluorescein 5-Isothiocyanate, Isomer 1 (~90%)

    Controlled Product
    CAS:

    Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens.
    References Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958), Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1972),

    Formula:C21H11NO5S
    Purity:~90%
    Color and Shape:Neat
    Molecular weight:389.38

    Ref: TR-F485720

    100mg
    122.00€
    500mg
    137.00€
    1g
    175.00€
  • Fluorescein 5-Isothiocyanate, Isomer 1

    CAS:
    Formula:C21H11NO5S
    Molecular weight:389.39

    Ref: ST-EA-CP-F116033

    10mg
    To inquire
    25mg
    To inquire
    50mg
    To inquire
    100mg
    To inquire