CAS 3326-34-9

5-Aminofluorescein

Description:

5-Aminofluorescein is a synthetic organic compound that belongs to the class of fluorescein derivatives. It is characterized by its bright green fluorescence, which makes it useful in various applications, including biological imaging and as a fluorescent tracer. The molecular structure features an amino group (-NH2) that enhances its reactivity and solubility in aqueous solutions. This compound typically exhibits strong absorption in the ultraviolet-visible spectrum, with fluorescence emission occurring in the visible range, making it valuable for fluorescence microscopy and other analytical techniques. 5-Aminofluorescein is also known for its potential use in bioconjugation and as a marker in cellular studies due to its ability to bind to biomolecules. Additionally, it is important to handle this compound with care, as it may pose health risks if ingested or inhaled, and appropriate safety measures should be taken during its use in laboratory settings. Overall, 5-Aminofluorescein is a versatile compound with significant utility in scientific research and diagnostics.

Formula: C₂₀H₁₃NO₅

InChI: InChI=1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2

InChI key: InChIKey=GZAJOEGTZDUSKS-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC(N)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Synonyms:

• 4-Aminofluorescein
• 5-Amino-2-(3-Hydroxy-6-Oxoxanthen-9-Yl)Benzoic Acid
• 5-Amino-3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
• 5-Aminofluorescein
• 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid
• 5-amino-6'-hydroxy-3H-spiro[2-benzofuran-1,9'-xanthene]-3,3'(9a'H)-dione
• Fluorescein amine isomer 1
• Fluorescein, 5-amino-
• Fluoresceinamine-I
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 5-amino-3′,6′-dihydroxy-

5-Aminofluorescein (isomer I)

CAS: 3326-34-9

Formula: C₂₀H₁₃NO₅

Purity: >95.0%(T)(HPLC)

Color and Shape: Orange to Brown to Dark red powder to crystal

Molecular weight: 347.33

Ref: 3B-A0306

Fluoresceinamine, isomer I, 96%

CAS: 3326-34-9

A fluorescent labeling reagent

Formula: C₂₀H₁₃NO₅

Purity: 96%

Molecular weight: 347.32

Ref: 02-J62175

5-Aminofluorescein

CAS: 3326-34-9

Formula: C₂₀H₁₃NO₅

Purity: 95%

Color and Shape: Solid

Molecular weight: 347.3209

Ref: IN-DA00BXOF

Fluoresceinamine, isomer I

CAS: 3326-34-9

Fluoresceinamine, isomer I

Formula: C₂₀H₁₃NO₅

Purity: 95%

Color and Shape: Solid

Molecular weight: 347.32092

Ref: 54-BIF4011

5-Aminofluorescein

CAS: 3326-34-9

5-Aminofluorescein

Purity: ≥98%

Molecular weight: 347.32g/mol

Ref: 54-BUP17598

Fluoresceinamine, isomer I

CAS: 3326-34-9

Formula: C₂₀H₁₃NO₅

Purity: (HPLC) ≥ 97.0%

Color and Shape: Dark red to brown crystalline powder

Molecular weight: 347.32

Ref: 7W-GT9206

5-Aminofluorescein

CAS: 3326-34-9

5-Aminofluorescein (5-AF) is a fluorescent marker bound to human serum albumin; excites at 490 nm, emits at 515 nm.

Formula: C₂₀H₁₃NO₅

Purity: 98.59%

Color and Shape: Dark Brown Solid

Molecular weight: 347.32

Ref: TM-T18889

5-Aminofluorescein

CAS: 3326-34-9

5-Aminofluorescein is a fluorescence probe that is used to detect the presence of reactive groups on basic fibroblast cells. 5-Aminofluorescein reacts with an ionotropic gelation agent and forms a fluorescent gel when it contacts cells. The fluorescent gel penetrates the cell membrane and binds to enzymes, which will cause the release of cellular energy in the form of heat. This energy can be measured by calorimetry or by measuring the change in fluorescence. 5-Aminofluorescein has been shown to have significant cytotoxicity against malignant brain tumor cells, which may be due to its ability to penetrate into these cells and bind to DNA. It also has antimicrobial properties and can be used as a titration indicator for certain drugs, such as antibiotics.

Formula: C₂₀H₁₃NO₅

Purity: Min. 95 Area-%

Color and Shape: Powder

Molecular weight: 347.32 g/mol

Ref: 3D-FA02871

5-Aminofluorescein

CAS: 3326-34-9

Formula: C₂₀H₁₃NO₅

Purity: 95%

Color and Shape: Solid, Blackish purple powder

Molecular weight: 347.326

Ref: 10-F223256

Fluoresceinamine, Isomer 1

Controlled Product

CAS: 3326-34-9

Stability Light and Moisture Sensitive
Applications Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. Dyes and metabolites.
References Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958), Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1972),

Formula: C₂₀H₁₃NO₅

Color and Shape: Neat

Molecular weight: 347.32

Ref: TR-F485300