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CAS 50931-35-6

:

Ac-Lys-pNA . HCl

Description:
Ac-Lys-pNA·HCl, or N-acetyl-Lysine p-nitroanilide hydrochloride, is a synthetic compound commonly used in biochemical research, particularly in enzyme assays. It features an acetylated lysine residue linked to a p-nitroaniline moiety, which serves as a chromogenic substrate. This compound is typically utilized to measure the activity of proteolytic enzymes, such as trypsin or chymotrypsin, which cleave peptide bonds. Upon cleavage, the p-nitroaniline is released, leading to a measurable color change that can be quantified spectrophotometrically. Ac-Lys-pNA·HCl is soluble in water and exhibits stability under appropriate storage conditions. Its hydrochloride form enhances solubility and stability in aqueous solutions. The compound is characterized by its ability to provide a clear and sensitive readout for enzyme activity, making it a valuable tool in both research and diagnostic applications. Safety precautions should be observed when handling this compound, as with many chemical substances, to avoid potential hazards associated with its use.
Formula:C14H20N4O4·HCl
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