CAS 61135-33-9

5-Ethynyl-2′-deoxyuridine

Description:

5-Ethynyl-2′-deoxyuridine (EdU) is a nucleoside analog of thymidine, characterized by the presence of an ethynyl group at the 5-position of the uracil base. This modification enhances its incorporation into DNA during replication, making it a valuable tool in molecular biology, particularly for studying cell proliferation and DNA synthesis. EdU is often used in click chemistry applications, allowing for the selective labeling of newly synthesized DNA in live cells. It is soluble in aqueous solutions, which facilitates its use in various biological assays. The compound exhibits low toxicity, making it suitable for in vivo studies. Additionally, EdU can be detected using fluorescent labeling techniques, enabling researchers to visualize and quantify cell division. Its unique properties make it an essential reagent in cancer research, developmental biology, and other fields where understanding cellular dynamics is crucial. Overall, EdU serves as a powerful tool for investigating cellular processes and has broad applications in both basic and applied research.

Formula: C₁₁H₁₂N₂O₅

InChI: InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1

InChI key: InChIKey=CDEURGJCGCHYFH-DJLDLDEBSA-N

SMILES: O=C1N([C@@H]2O[C@H](CO)[C@@H](O)C2)C=C(C#C)C(=O)N1

Synonyms:

• 2'-Deoxy-5-ethynyluridine
• 2,4(1H,3H)-pyrimidinedione, 1-(2-deoxypentofuranosyl)-5-ethynyl-
• 5-Ethynyl-2'-Deoxyuridine
• Uridine, 2'-Deoxy-5-Ethynyl-
• EdU

5-Ethynyl-2'-deoxyuridine

CAS: 61135-33-9

Formula: C₁₁H₁₂N₂O₅

Purity: >98.0%(T)(HPLC)

Color and Shape: White to Orange to Green powder to crystal

Molecular weight: 252.23

Ref: 3B-E1057

Uridine, 2'-deoxy-5-ethynyl-

CAS: 61135-33-9

Formula: C₁₁H₁₂N₂O₅

Purity: 98%

Color and Shape: Solid

Molecular weight: 252.2234

Ref: IN-DA00IATB

5''-Ethynyl-2''-deoxyuridine

CAS: 61135-33-9

Formula: C₁₁H₁₂N₂O₅

Purity: ≥ 98.0%

Color and Shape: White to off-white powder

Molecular weight: 252.23

Ref: 7W-GN5170

5-Ethynyl-2'-deoxyuridine

CAS: 61135-33-9

5-Ethynyl-2'-deoxyuridine

Purity: 98%

Color and Shape: Solid

Molecular weight: 252.22g/mol

Ref: 54-OR918030

5-Ethynyl-2'-deoxyuridine

CAS: 61135-33-9

5-Ethynyl-2'-deoxyuridine

Purity: ≥98%

Molecular weight: 252.22g/mol

Ref: 54-BUP15113

5-Ethynyl-2'-deoxyuridine

CAS: 61135-33-9

EdU, a thymidine analog, tracks DNA synthesis via click chemistry and is a PROTAC linker.

Formula: C₁₁H₁₂N₂O₅

Purity: 98.1% - >99.99%

Color and Shape: Solid

Molecular weight: 252.22

Ref: TM-T17341

5-Ethynyl-2’-deoxyuridine

Controlled Product

CAS: 61135-33-9

Applications 5-Ethynyl-2’-deoxyuridine is an extensively used nucleoside analog used as a probe for DNA synthesis. 5-Ethynyl-2’-deoxyuridine is known as an toxic anti-metabolite, which potentially causes DNA instabiliy, necrosis and cell-cycle arrest, therefore it is often used for short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required.
References Neef, A.B., et al.: P. Natl. Acad. Sci.., 108, 20404 (2011); Gristwood, T., et al.: Nucleic. Acids. Rsch., 40, 5487 (2012);

Formula: C₁₁H₁₂N₂O₅

Color and Shape: Neat

Molecular weight: 252.22

Ref: TR-E932175

EdU

CAS: 61135-33-9

2'-Deoxy-5-ethynyluridine (5-EdU) is a modified thymidine analogue containing a terminal alkyne group, which is used for nucleotide labelling. 5-Edu is easily taken up by cells and incorporated by metabolic activation during DNA synthesis. By adding an azide conjugated to a fluorophore or a biotin moiety, a click reaction occurs from the labelled nucleotide linked by a triazole group. 5-Edu are employed in cell proliferation assays, to study DNA and RNA localisation, structure and dynamics, in the synthesis of aptamers with the click-SELEX method, and in other applications such as the preparation of oligonucleotide microarrays and nanoelectronic devices.

Formula: C₁₁H₁₂N₂O₅

Purity: Min. 98 Area-%

Color and Shape: Powder

Molecular weight: 252.23 g/mol

Ref: 3D-NE08701

5-Ethynyl-2′-deoxyuridine

CAS: 61135-33-9

Formula: C₁₁H₁₂N₂O₅

Purity: 95%

Color and Shape: Solid

Molecular weight: 252.226

Ref: 10-F463473

5-Ethynyl-2’-deoxyuridine-d1

Controlled Product

CAS: 61135-33-9

Formula: C₁₁DH₁₁N₂O₅

Color and Shape: Neat

Molecular weight: 253.23

Ref: TR-E932177