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CAS 72-57-1

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Trypan blue

Description:
Trypan blue is a synthetic dye commonly used in biological and medical research, particularly for cell viability assays. It is a diazo dye that appears as a dark blue powder or solution and is soluble in water. The dye selectively penetrates dead cells, allowing researchers to distinguish between viable and non-viable cells under a microscope. This characteristic is due to the compromised membrane integrity of dead cells, which allows the dye to enter, whereas live cells exclude it. Trypan blue is often used in conjunction with hemocytometers for counting cells in culture. While it is generally considered safe for laboratory use, it is important to handle it with care, as it can be a potential irritant and is classified as a possible carcinogen. In addition to its applications in cell biology, Trypan blue has also been used in histology and as a staining agent in various diagnostic procedures. Overall, its ability to provide a visual distinction between live and dead cells makes it a valuable tool in cell biology research.
Formula:C34H28N6O14S4·4Na
InChI:InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;
InChI key:InChIKey=UBHCTCVOHFAILV-UHFFFAOYSA-N
SMILES:OC=1C2=C(C=C(S(=O)(=O)O)C1N=NC3=C(C)C=C(C=C3)C4=CC(C)=C(N=NC=5C(S(=O)(=O)O)=CC6=C(C5O)C(N)=CC(S(=O)(=O)O)=C6)C=C4)C=C(S(=O)(=O)O)C=C2N.[Na]
Synonyms:
  • 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-, sodium salt (1:4)
  • 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxy-, tetrasodium salt
  • 3,3'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphtalene-2,7-disulfonate] de tetrasodium
  • 3,3'-[(3,3'-Dimetil[1,1'-Bifenil]-4,4'-Diil)Bis(Azo)]Bis[5-Amino-4-Hidroxinaftaleno-2,7-Disulfonato] De Tetrasodio
  • Amanil Sky Blue R
  • Bencidal Blue 3B
  • Benzamine blue
  • Benzanil Blue 3BN
  • Benzo Blue 3BS
  • Benzo blue
  • See more synonyms
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Found 14 products.
  • Direct Blue 14

    CAS:
    Formula:C34H24N6Na4O14S4
    Color and Shape:Amber to Dark green to Black powder to crystal
    Molecular weight:960.79

    Ref: 3B-T0556

    25g
    42.00€
  • Trypan Blue, dye content >60%

    CAS:
    <p>Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell clust</p>
    Formula:C34H24N6Na4O14S4
    Purity:60%
    Color and Shape:Dark purple to dark brown or black, Powder
    Molecular weight:960.79

    Ref: 02-A18600

    25g
    38.00€
    100g
    105.00€
    500g
    To inquire
  • Ref: IN-DA00IC7M

    1g
    27.00€
    5g
    47.00€
    25g
    72.00€
    100g
    158.00€
  • Trypan Blue (C.I. 23850); 0.4% solution

    CAS:
    Formula:C34H24N6Na4O14S4
    Color and Shape:Blue to very dark blue liquid
    Molecular weight:960.82

    Ref: 7W-GT0822

    100ml
    63.00€
  • Trypan Blue

    CAS:
    <p>Trypan Blue</p>
    Purity:80%
    Molecular weight:960.81g/mol

    Ref: 54-OR1015400

    1g
    32.00€
    5g
    30.00€
    25g
    57.00€
    100g
    163.00€
    500g
    683.00€
    2.5kg
    2,971.00€
  • Diphenyl Blue

    CAS:
    <p>Diphenyl Blue</p>
    Molecular weight:960.81g/mol

    Ref: 54-BUP19760

    5g
    To inquire
  • Trypan Blue (C.I. 23850)

    CAS:
    Formula:C34H24N6Na4O14S4
    Color and Shape:Dark green, brown or black powder
    Molecular weight:960.82

    Ref: 7W-GT3970

    25g
    42.00€
    100g
    99.00€
  • Trypan Blue

    CAS:
    Formula:C34H28N6NaO14S4
    Molecular weight:895.86

    Ref: 4Z-D-164054

    5mg
    To inquire
    10mg
    To inquire
    25mg
    To inquire
    50mg
    To inquire
    100mg
    To inquire
  • Diphenyl Blue

    CAS:
    <p>Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.</p>
    Formula:C34H24N6Na4O14S4
    Purity:≥98%
    Color and Shape:Blueish Grey Powder
    Molecular weight:960.81
  • Trypan Blue (Technical Grade)

    CAS:
    <p>Applications Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.<br>References Bonnefille, P. et al. 2010. Fundam Clin Pharmacol. PMID: 21121944; Han, T.H. et al. 2010. J Acupunct Meridian Stud. 3(4): 241-248. PMID: 21185538; Saadatnia, G. et al. 2010. Trop Biomed. 27(1): 125-130. PMID: 20562822; Hani, H. et al. 2010. Xenotransplantation. 17(6): 469-480. PMID: 21158948<br></p>
    Formula:C34H24N6O14S4·4Na
    Color and Shape:Neat
    Molecular weight:960.80

    Ref: TR-T895523

    1g
    91.00€
    5g
    180.00€
    10g
    312.00€
  • Trypan blue

    CAS:
    <p>Dye used in microscopy for assessment of cell viability</p>
    Formula:C34H24N6O14S4Na4
    Color and Shape:Powder
    Molecular weight:960.81 g/mol

    Ref: 3D-FT47140

    1kg
    806.00€
    2kg
    1,356.00€
    100g
    184.00€
    250g
    339.00€
    500g
    500.00€
  • Trypan Blue 0.4% Solution

    CAS:
    Color and Shape:Dark blue, Liquid

    Ref: SR-12788

    50ml
    32.00€
    125ml
    26.00€
  • Trypan Blue

    CAS:
    Formula:C34H24N6Na4O14S4
    Color and Shape:Brown to black, Powder
    Molecular weight:960.80

    Ref: SR-58026

    25g
    28.00€
    100g
    55.00€
  • Trypan Blue for tissue culture

    CAS:
    Formula:C34H24N6Na4O14S4
    Color and Shape:Brown to black, Powder
    Molecular weight:960.80

    Ref: SR-31591

    25g
    27.00€