CAS 72-57-1
:Trypan blue
Description:
Trypan blue is a synthetic dye commonly used in biological and medical research, particularly for cell viability assays. It is a diazo dye that appears as a dark blue powder or solution and is soluble in water. The dye selectively penetrates dead cells, allowing researchers to distinguish between viable and non-viable cells under a microscope. This characteristic is due to the compromised membrane integrity of dead cells, which allows the dye to enter, whereas live cells exclude it. Trypan blue is often used in conjunction with hemocytometers for counting cells in culture. While it is generally considered safe for laboratory use, it is important to handle it with care, as it can be a potential irritant and is classified as a possible carcinogen. In addition to its applications in cell biology, Trypan blue has also been used in histology and as a staining agent in various diagnostic procedures. Overall, its ability to provide a visual distinction between live and dead cells makes it a valuable tool in cell biology research.
Formula:C34H28N6O14S4·4Na
InChI:InChI=1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;
InChI key:InChIKey=UBHCTCVOHFAILV-UHFFFAOYSA-N
SMILES:OC=1C2=C(C=C(S(=O)(=O)O)C1N=NC3=C(C)C=C(C=C3)C4=CC(C)=C(N=NC=5C(S(=O)(=O)O)=CC6=C(C5O)C(N)=CC(S(=O)(=O)O)=C6)C=C4)C=C(S(=O)(=O)O)C=C2N.[Na]
Synonyms:- 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-, sodium salt (1:4)
- 2,7-Naphthalenedisulfonic acid, 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxy-, tetrasodium salt
- 3,3'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphtalene-2,7-disulfonate] de tetrasodium
- 3,3'-[(3,3'-Dimetil[1,1'-Bifenil]-4,4'-Diil)Bis(Azo)]Bis[5-Amino-4-Hidroxinaftaleno-2,7-Disulfonato] De Tetrasodio
- Amanil Sky Blue R
- Bencidal Blue 3B
- Benzamine blue
- Benzanil Blue 3BN
- Benzo Blue 3BS
- Benzo blue
- Blue 3B
- Blue EMB
- Brasilamina Blue 3B
- Chloramine Blue 3B
- Chrome Leather Blue 3B
- Congo blue
- Cresotine Blue 3B
- Diamine Blue 3B
- Diamineblue
- Diaphtamine Blue TH
- Diazine Blue 3B
- Diazol Blue 3B
- Diphenyl Blue
- Diphenyl Blue 3B
- Direct Blue 14
- Direct Blue 3B
- Direct Blue M 3B
- Hispamin Blue 3BX
- Naphthylamine blue
- Niagara Blue
- Niagara Blue 3B
- Paramine Blue 3B
- Pontamine Blue 3BX
- Sodium ditolyldisazobis-8-amino-1-naphthol-3,6-disulfonate
- Tetranatrium-3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalin-2,7-disulfonat]
- Tetrasodium 3,3'-[(3,3'-Dimethyl[1,1'-Biphenyl]-4,4'-Diyl)Bis(Azo)]Bis[5-Amino-4-Hydroxynaphthalene-2,7-Disulfonate]
- Tetrasodium 3,3'-[(3,3'-Dimethyl[1,1'-Biphenyl]-4,4'-Diyl)Bis(Azo)]Bis[5-Amino-4-Hydroxynaphthalene-2,7-Disulphonate]
- Trypan (Congo) Blue
- Trypan Blue (Dye Cont. Ca. 60%)
- Trypan Blue BPC
- Trypan Blue solution
- Trypane blue
- tetrasodium (3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonate)
- tetrasodium 3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)
- C.I. Direct Blue 14, tetrasodium salt
- C.I. Direct Blue 14, tetrasodium salt (8CI)
- C.I. Direct Blue 14
- C.I. Direct Blue 14, tetrasodium salt
- C.I. 23850
- Direct Blue 14 (C.I.)
- See more synonyms
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Direct Blue 14
CAS:Formula:C34H24N6Na4O14S4Color and Shape:Amber to Dark green to Black powder to crystalMolecular weight:960.79Trypan Blue, dye content >60%
CAS:Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. Used in dye exclusion studies in cell culture. A viability dye for quantifying live versus dead cells with permeable membranes, blue. It is also used to identify islet cell clustFormula:C34H24N6Na4O14S4Purity:60%Color and Shape:Dark purple to dark brown or black, PowderMolecular weight:960.79Trypan Blue
CAS:Trypan BlueFormula:C34H24N6Na4O14S4Purity:80%Color and Shape:SolidMolecular weight:960.81Trypan Blue (C.I. 23850)
CAS:Formula:C34H24N6Na4O14S4Color and Shape:Dark green, brown or black powderMolecular weight:960.82Trypan Blue (C.I. 23850); 0.4% solution
CAS:Formula:C34H24N6Na4O14S4Color and Shape:Blue to very dark blue liquidMolecular weight:960.822,7-Naphthalenedisulfonic acid,3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxy-, tetrasodium salt
CAS:Formula:C34H24N6Na4O14S4Purity:70%Color and Shape:SolidMolecular weight:960.8052Trypan Blue (Technical Grade)
CAS:Applications Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. Dyes and metabolites.
References Bonnefille, P. et al. 2010. Fundam Clin Pharmacol. PMID: 21121944; Han, T.H. et al. 2010. J Acupunct Meridian Stud. 3(4): 241-248. PMID: 21185538; Saadatnia, G. et al. 2010. Trop Biomed. 27(1): 125-130. PMID: 20562822; Hani, H. et al. 2010. Xenotransplantation. 17(6): 469-480. PMID: 21158948Formula:C34H24N6O14S4·4NaColor and Shape:NeatMolecular weight:960.8Diphenyl Blue
CAS:Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.Formula:C34H24N6Na4O14S4Purity:96.01%Color and Shape:Black SolidMolecular weight:960.81Sodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)
CAS:Sodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)Formula:C34H24N6Na4O14S4Purity:70% (dye content)Molecular weight:960.81Trypan blue
CAS:Dye used in microscopy for assessment of cell viability
Formula:C34H24N6O14S4Na4Color and Shape:PowderMolecular weight:960.81 g/molTrypan Blue
CAS:Formula:C34H24N6Na4O14S4Color and Shape:Brown to black, Crystalline powderMolecular weight:960.80Trypan Blue for tissue culture
CAS:Formula:C34H24N6Na4O14S4Color and Shape:Brown to black, PowderMolecular weight:960.80
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