CAS 72040-63-2

NHS-LC-Biotin

Description:

NHS-LC-Biotin, with the CAS number 72040-63-2, is a biotin derivative commonly used in biochemical applications, particularly in labeling and detection assays. It features a N-hydroxysuccinimide (NHS) ester, which allows for efficient conjugation to amine-containing molecules, such as proteins and peptides, under mild conditions. The "LC" in its name indicates that it has a long carbon chain, enhancing its solubility and stability in various biological environments. NHS-LC-Biotin is characterized by its ability to form stable amide bonds with primary amines, making it a valuable tool in bioconjugation techniques. This compound is often utilized in applications such as affinity purification, immunoassays, and in the development of biotinylated probes for various analytical techniques. Its biotin component allows for strong binding to avidin or streptavidin, facilitating the detection and isolation of biomolecules. Overall, NHS-LC-Biotin is an essential reagent in molecular biology and biochemistry for studying protein interactions and functions.

Formula: C₂₀H₃₀N₄O₆S

InChI: InChI=1S/C20H30N4O6S/c25-15(7-4-3-6-14-19-13(12-31-14)22-20(29)23-19)21-11-5-1-2-8-18(28)30-24-16(26)9-10-17(24)27/h13-14,19H,1-12H2,(H,21,25)(H2,22,23,29)/t13-,14-,19-/m0/s1

InChI key: InChIKey=UVGHPGOONBRLCX-NJSLBKSFSA-N

SMILES: C(CCCC(NCCCCCC(ON1C(=O)CCC1=O)=O)=O)[C@H]2[C@@]3([C@](CS2)(NC(=O)N3)[H])[H]

Synonyms:

• (+)-Biotinamidocaproate N-hydroxysuccinimidyl ester
• 1H-Thieno[3,4-d]imidazole-4-pentanamide, N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]hexahydro-2-oxo-, (3aS,4S,6aR)-
• 1H-Thieno[3,4-d]imidazole-4-pentanamide, N-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]hexahydro-2-oxo-, [3aS-(3aα,4β,6aα)]-
• 5-(N-Succinimidyloxycarbonyl)pentyl d-biotinamide
• 6-[(Biotinyl)amino]hexanoic acid N-hydroxysuccinimide ester
• <span class="text-smallcaps">D</span>-(+)-Biotinyl-ε-aminocaproic acid N-hydroxysuccimide ester
• B 1582
• Biotin-LC-NHS
• Biotin-X, SE
• Biotinamidocaproate N-hydroxysuccinimide ester
• Biotinamidohexanoic acid N-hydroxysuccinimide ester
• EZ-Link NHS-LC-Biotin
• Enzotin
• Hexanoic acid, 6-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-, 2,5-dioxo-1-pyrrolidinyl ester
• N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester
• N-Hydroxysuccinimidyl 6-(biotinamido)hexanoate
• N-Hydroxysuccinimidyl biotinamidocaproate
• N-Succinimidyl N-biotinyl-6-aminocaproate
• N-[5-(Succinimidyloxycarbonyl)pentyl]biotin amide
• N-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
• N-{6-[(2,5-dioxopyrrolidin-1-yl)oxy]-6-oxohexyl}-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide

N-Succinimidyl 6-Biotinamidohexanoate

CAS: 72040-63-2

Formula: C₂₀H₃₀N₄O₆S

Purity: >97.0%(HPLC)

Color and Shape: White to Almost white powder to crystal

Molecular weight: 454.54

Ref: 3B-S0490

2,5-Dioxopyrrolidin-1-yl 6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoate

CAS: 72040-63-2

Formula: C₂₀H₃₀N₄O₆S

Purity: 96%

Color and Shape: Solid

Molecular weight: 454.5404

Ref: IN-DA005NUV

Biotin-C5-NHS Ester

CAS: 72040-63-2

Biotin-C5-NHS Ester is an alkyl/ether-based linker for PROTAC synthesis[1].

Formula: C₂₀H₃₀N₄O₆S

Color and Shape: White Solid

Molecular weight: 454.54

Ref: TM-T14576

Biotin-X-NHS

CAS: 72040-63-2

Ref: 7W-GW6213

Succinimidyl-6-(biotinamido)hexanoate

CAS: 72040-63-2

Succinimidyl-6-(biotinamido)hexanoate

Formula: C₂₀H₃₀N₄O₆S

Purity: By hplc: 99.9% (Typical Value in Batch COA)

Color and Shape: white crystalline powder

Molecular weight: 454.54g/mol

Ref: 54-BIB104

Succinimidyl-6-(biotinamido) Hexanoate

Controlled Product

CAS: 72040-63-2

Stability Moisture Sensitive
Applications Succinimidyl-6-(biotinamido) Hexanoate is a biotinylation reagent incorporating an aminocaproyl “spacer”. This can reduce the steric hindrance in binding avidin to some biotinylated compounds when used to biotinylate both the antibody and the carrier in the Protein Avidin-Biotin Capture System. This system avoids the direct interaction of a captured antibody with solid surfaces, such as plastics, which may reduce antigenicity. It has also been used to enhance the detection of DNA on nitrocellulose. Has also been used as a cell surface labelling reagent
References Hofmann, K., et al.: Biochemistry, 21, 978 (1982); Leary, J.J., et al.: P. Natl. Acad. Sci. USA, 80, 4045, (1983); Suter, M., et al.: Immunology Letters, 13, 313 (1986); Gretch, D.R., et al.: Anal. Biochem., 163, 270 (1987), Burgess, T.L., et al.: J. Biol. Chem., 269 (32), 19188 (1995), Schumacher, T.N.M., et al.: Science, 271, 1854 (1996)

Formula: C₂₀H₃₀N₄O₆S

Color and Shape: Off White Crystalline Powder

Molecular weight: 454.54

Ref: TR-S690250

Biotin-X, succinimidyl ester

CAS: 72040-63-2

M03680 - Biotin-X, succinimidyl ester

Formula: C₂₀H₃₀N₄O₆S

Purity: 96%

Color and Shape: Solid, Crystalline Powder

Molecular weight: 454.54

Ref: 10-M03680