CAS 7365-45-9: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Description: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, commonly known as HEPES, is a zwitterionic buffering agent widely used in biological and biochemical research. It has a pKa value around 7.5, making it particularly effective for maintaining physiological pH in cell culture and biochemical assays. HEPES is soluble in water and exhibits good stability under physiological conditions, which is crucial for experiments requiring consistent pH levels. Its structure features a piperazine ring, a sulfonic acid group, and a hydroxyethyl side chain, contributing to its buffering capacity. HEPES is non-toxic to cells, making it suitable for various applications, including protein purification and enzyme assays. Additionally, it is less prone to interfere with biological processes compared to other buffers, such as phosphate buffers. However, it is important to note that HEPES can absorb UV light, which may affect certain spectroscopic analyses. Overall, HEPES is a versatile and widely utilized buffer in laboratory settings, particularly in the fields of molecular biology and biochemistry.

Formula: C₈H₁₈N₂O₄S

InChI: InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key: InChIKey=JKMHFZQWWAIEOD-UHFFFAOYSA-N

SMILES: O=S(=O)(O)CCN1CCN(CCO)CC1

Synonyms:

• 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
• 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
• 2-[4-(2-Hydroxyethyl)-1-Piperazinyl]Ethanesulfonic Acid
• 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)Piperazin-4-Ium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)Piperazinediium-1-Yl]Ethanesulfonate
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
• 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
• 2-[4-(Hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
• 4-(2-Hydroxyethy1)-1-piperazineethanesulfonic acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid
• 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonicacid
• 4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
• 4-(2-Hydroxyethyl)Piperazin-1-Ylethanesulphonic Acid
• 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid
• 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
• 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
• Acide 4-(2-Hydroxyethyl)Piperazine-1-Ylethanesulfonique
• Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
• Acido 4-(2-Hidroxietil)Piperazin-1-Iletanosulfonico
• Hepes
• Labotest-Bb Lt00138109
• Labotest-Bb Lt00233156
• N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Molecular Biology Grade
• N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonic acid), Tissue Culture Tested
• N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
• N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
• Nsc 166663
• Piperazine, 1-(Ethylsulfonic Acid)-4- (2-Hydroxyethyl)-
• Tvz 7
• Was 13

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: >99.0%(T)
• Color and Shape: White to Almost white powder to crystal
• Molecular weight: 238.30

HEPES, 1.0M buffer soln., pH 6.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. …

HEPES, 1.0M buffer soln., pH 9.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. …

HEPES Buffer, 0.2M buffer soln., pH 7.4, low endotoxin

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. …

HEPES, 99%

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological …

• Formula: C₈H₁₈N₂O₄S
• Purity: 99%
• Color and Shape: White, Crystals or powder or crystalline powder
• Molecular weight: 238.30

HEPES, 0.5M buffer soln., pH 7.0

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. …

HEPES, 1.0M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Liquid, Clear colorless
• Molecular weight: 238.30

HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Molecular weight: 238.30

HEPES, 0.5M buffer soln., pH 8.0

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and …

HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Liquid, Clear colorless
• Molecular weight: 238.30

HEPES, 0.5M buffer soln., pH 7.5

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It …

HEPES-buffered saline, pH 7.0 (5X)

CAS: 7365-45-9

HEPES is widely used in cell culture. HEPES is used to neutralize the complex proteins …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Clear, colorless liquid
• Molecular weight: 238.30

HEPES, 0.5M buffer soln., pH 8.5

CAS: 7365-45-9

HEPES has been utilized extensively for research and commercial applications in the cell culture and …

HEPES, 0.5M buffer soln., pH 7.6

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It …

HEPES, ≥99% (Dry Basis), Ultrapure

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: 99%
• Color and Shape: White crystalline powder
• Molecular weight: 238.30

HEPES-buffered saline, pH 7.0 (2X for transfection)

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Clear colorless, Liquid
• Molecular weight: 238.30

HEPES-buffered saline, pH 6.5 (5X)

CAS: 7365-45-9

HEPES is typically used in cell culture at concentration between 5mM to 30 mM. It …

• Formula: C₈H₁₈N₂O₄S
• Molecular weight: 238.30

HEPES, 1.0M buffer soln., pH 7.0

CAS: 7365-45-9

HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological …

• Formula: C₈H₁₈N₂O₄S
• Color and Shape: Clear colorless, Liquid
• Molecular weight: 238.30

HEPES, 1.0M buffer soln., pH 8.5

CAS: 7365-45-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. …

2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid

CAS: 7365-45-9

• Formula: C₈H₁₈N₂O₄S
• Purity: 97%
• Color and Shape: Solid
• Molecular weight: 238.3045