Enzymes

BioLit QuickRxn EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C ( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration : 20U/mcl

Isoschizomers: FauNDI Source: Neisseria denitrificans 5'-CA↓TA TG-3' 3'-GT AT↑AC-5

PstI is an endonuclease enzyme isolated from strains of Providencia stuartii, recombinant, E.coli and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is CTGCAG, which, as the complementary sequence is GACGTC, has rotational symmetry.

Isoschizomers: AhlI, BcuI Sphaerotilus species A↓CTAG T-3' 3'-T GATC↑A-5'

MspCI is an endonuclease enzyme isolated from strains of Micrococcus species and is part of the restriction modification system. It is an isoschizomer of AflII. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CTTAAG, which, as the complementary sequence is GAATTC, has rotational symmetry.

Isoschizomers: HindII Source: Haemophilus influenzae Rc 5'-GTY↓RAC-3' 3'-CAR↑YTG-5

<p>SacI is an Isoschizomers of SstI,<br> Neoschizomers: Ecl136II, EcoICRI<br> Source: Streptomyces Stanford<br> 5'-G AGCT↓C-3'<br> 3'-C↑TCGA G-5' </p>

SalI is an endonuclease enzyme isolated from strains of Streptomyces albus G and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GTCGAC, which, as the complementary sequence is CAGCTG, has rotational symmetry.

QuickRxn HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. In molecular biology it is used as a restriction enzyme of 35.8kDa. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is AAGCTT, which, as the complementary sequence is TTCGAA, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm or CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration: 20U/mcl

HinfI is an endonuclease enzyme isolated from strains of Haemophilus influenzae Rf, recombinant, E. coli and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3&apos; overhang. The nucleic acid sequence where the enzyme cuts is GANTC, which, as the complementary sequence is CTNAG, has rotational symmetry.

An Unique buffer that can be used for most Restriction enzyme reaction set-ups. This buffer aides in optimal digestion within the specified time with great efficiency

SmaI is an endonuclease enzyme isolated from strains of Serratia marcescens and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates blunt ends . The nucleic acid sequence where the enzyme cuts is CCCGGG, which, as the complementary sequence is GGGCCC.

XhoI is an Isoschizomer of SlaI. Source: Streptomyces lavendulae 5'-C↓TCGA G-3' 3'-G AGCT↑C-5'

<p>RsaI is an endonuclease enzyme isolated from strains of E.coli carrying the gene from Rhodopseudomonas sphaeroides and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is GTAC, which, as the complementary sequence is CATG, has rotational symmetry. </p>

<p>AluI is an endonuclease enzyme isolated from strains of Arthrobacter luteus, and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is AGCT which, as the complementary sequence is TCGA, has rotational symmetry. </p>

BglII is an endonuclease enzyme isolated from strains of Bacillus globigii lacking BglII and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme that creates sticky ends. The nucleic acid sequence where the enzyme cuts is AGATCT, which as the complementary sequence is TCTAGA.

BstEII is an endonuclease enzyme isolated from strains of Bacillus stearothermophilus , and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GGTNACC, which, as the complementary sequence is CCANTGG, has rotational symmetry.

<p>Isoschizomers: BfuCI, Bsp143I, BstENII, BstMBI, DpnII, Kzo9II, NdeII<br> Neoschizomers: BstKTI<br> Source: Moraxella bovis, recombinant, Streptomyces sp.<br> 5'-↓GATC-3'<br> 3'-CTAG↑-5' </p>

<p>This is an Isoschizomers of BshFI<br> Source :Haemophilus aegyptius<br> 5'-GG↓CC-3'<br> 3'-CC↑GG-5' </p>

<p>XbaI is an endonuclease enzyme isolated from strains of Xanthomonas badrii, and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is TCTAGA, which, as the complementary sequence is AGATCT, has rotational symmetry. </p>

EcoRV is an endonuclease enzyme isolated from strains of Escherichia.coli J62plg 74, and is part of the restriction modification system. It is also known as Eco321. In molecular biology it is used as a restriction enzyme. It creates blunt ends The nucleic acid sequence where the enzyme cuts is GATATC, which, as the complementary sequence is CTATAG, has rotational symmetry.

NotI is an endonuclease enzyme isolated from strains of Nocardia otitidis-caviarum and is part of the restriction modification system.In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is GCGGCCGC, which, as the complementary sequence is CGCCGGCG, has rotational symmetry.

KpnI is an endonuclease enzyme isolated from strains of Klebsiella pneumoniae Ok8, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is GGTACC, which, as the complementary sequence is CCATGG, has rotational symmetry.

BamHI is an endonuclease enzyme isolated from strains of B.amyloliquefaciens H, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATCC, which, as the complementary sequence is CTTAGG, has rotational symmetry.

EcoRI is an endonuclease enzyme isolated from strains of Escherichia. Coli RY13, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.