Enzyme Substrates

<p>Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a high-quality pNP enzyme substrate developed for the sensitive detection and quantification of Beta-L-fucosidase activity. The enzymatic hydrolysis of the substrate generates 4-nitrophenol, a yellow chromogenic component that can be conveniently measured spectrophotometrically, allowing for accurate determination of enzyme concentration and kinetics. This substrate finds applications in various research fields, diagnostics, and industrial enzyme production.</p>

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. This may also be used for in-gel assays</p>

<p>Chromogenic substrate for beta-galactosidase</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that can be used in the detection of various enzymes. This product has been tested for sterility and purity, and it has passed all tests. It is also free of any detectable levels of heavy metals or other contaminants. 4NP2AG6S is a high quality bioluminescent substrate with CAS No. 71498-92-5. It is a fluorescent compound that reacts with an enzyme to produce light. The reaction can be detected by measuring the emitted light intensity at 680 nm (max).</p>

<p>Chromogenic substrate for beta-glucosidase</p>

<p>Fluorogenic substrate for cathepsin B or aminopeptidase III. Yields a blue fluorescent solution upon cleavage.</p>

<p>Chromogenic α-maltosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate for β-D-galactosidase, yielding a blue precipitate upon cleavage.</p>

<p>2-O-(4-Nitrophenyl)-alpha-D-N-acetylneuraminic acid sodium salt is a chromogenic substrate used to detect the enzymatic activity of neuraminidase or sialidase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-O-(4-Nitrophenyl)-alpha-D-N-acetylneuraminic acid sodium salt is used in bacterial neuraminidase activity assays.</p>

<p>N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a high quality and highly purified substrate for enzymes that catalyze the release of light when they react with their specific substrates. It is used in a variety of applications, including diagnostics and food testing. NACBZLAMC hydrochloride is an excellent ligand for chemiluminescence reactions and can be used to detect the presence of various analytes. It can also be used as a staining agent to detect proteins on Western blots or to stain bacterial cultures for identification. This product has been shown to be effective against numerous bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa, as well as fungi like Candida albicans.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.</p>

<p>Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Luc-coliforms Na is a fluorogenic substrate that is used in the diagnosis of bacterial contamination. It is a conjugate of the enzyme luciferase and the substrate coelenterazine, which are combined in a buffer solution. Luc-coliforms Na is used to detect bacteria by adding it to a culture broth or other growth media. The bacteria will emit light as they break down the luc-coliforms Na and produce ATP, which can then be measured with an appropriate detector. Luc-coliforms Na can also be used as a stain for gram-negative rods, such as coliforms, e.g., Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis.</p>

<p>A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.</p>

<p>PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.</p>

<p>Resorufin N-acetyl-b-D-glucosamine is fluorogenic substrate for N-acetyl-beta-D-glucosaminidase (NAG).</p>

<p>Chromogenic substrate targeting alpha-L-arabinofuranosidase</p>

<p>Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.</p>