Enzyme Substrates
Enzyme substrates are molecules upon which enzymes act to catalyze chemical reactions. The substrate binds to the active site of the enzyme, where it undergoes a specific transformation to form the product of the reaction. Enzyme substrates are essential tools in research for studying enzyme kinetics, specificity, and mechanism of action. They are also used in diagnostic assays and biochemical studies. At CymitQuimica, we provide a diverse selection of high-quality enzyme substrates to support your research in enzymology and biochemistry.
Subcategories of "Enzyme Substrates"
Found 1526 products of "Enzyme Substrates"
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5-Bromo-3-indolyl-β-D-galactopyranoside-6-sulfate sodium salt
CAS:5-Bromo-3-indolyl-beta-D-galactopyranoside-6-sulfate sodium salt is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in sulfatase activity assays and for the diagnosis of Mucopolysaccharidosis IVA (Morquio A).Formula:C14H15BrNNaO9SPurity:Min. 95%Color and Shape:PowderMolecular weight:476.23 g/mol5-Bromo-4-chloro-3-indoxyl-1-acetate
CAS:5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).
Purity:Min. 95%Color and Shape:PowderMolecular weight:288.52 g/molHippurate hydrolase from Campylobacter jejuni, aqueous solution with glycerol
Hippurate hydrolase is an enzyme that catalyzes the hydrolysis of hippurate to glycine and taurine. It is a fluorogenic substrate with high purity, ligand, and high quality. Hippurate hydrolase is used as a diagnostic enzyme for detecting the presence of hippuric acid in urine. This enzyme can be conjugated to a variety of molecules, including fluorophores, biotin or haptens, for use in immunoassay or other applications.Formula:C1944H3000N516O573S18Purity:Min. 95%Molecular weight:24,542 g/mol4-Nitrophenyl-alpha-L-fucopyranoside
CAS:Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.Formula:C12H15NO7Purity:Min. 98 Area-%Molecular weight:285.26 g/mol4-Chloro-1-naphthol
CAS:Substrate for horseradish peroxidase
Formula:C10H7ClOPurity:Min. 96.0 Area-%Color and Shape:Brown PowderMolecular weight:178.61 g/molAldol® 455 alpha-D-glucopyranoside - Biosynth Patent: EP 2427431 and US 8940909
Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.
Formula:C27H24ClNO7Purity:Min. 95 Area-%Color and Shape:PowderMolecular weight:509.93 g/mol5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside
5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).Formula:C13H13BrClNO5Purity:Min. 98.0 Area-%Color and Shape:PowderMolecular weight:378.6 g/molAldol® 484 beta-D-glucopyranoside, Biosynth Patent: EP 2427431 and US 8940909
CAS:Aldol® 484 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by bacteria producing beta-glucosidase in liquid or solid media, yielding intense red color and green fluorescent signal. Aldol® 484 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.Formula:C26H24ClFN2O7Purity:Min. 95%Color and Shape:PowderMolecular weight:530.93 g/mol5-Bromo-4-chloro-3-indoxyl phosphate, dipotassium salt
CAS:Also known as BCIP; can be used for visualization of phosphatase labelled antibodies on Western blots. This method quickly became extremely popular in diagnostics and biology research.Formula:C8H4BrClK2NO4PColor and Shape:Brown Off-White PowderMolecular weight:402.65 g/molL-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt
CAS:L-Phenylalanine 7-amido-4-methylcoumarin trifluoroacetate salt is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.Formula:C19H18N2O3•C2HF3O2Purity:Min. 98 Area-%Color and Shape:White PowderMolecular weight:436.38 g/molIsopropyl-β-D-thiogalactopyranoside - non-animal origin
CAS:A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.Formula:C9H18O5SPurity:Min. 99.0 Area-%Molecular weight:238.3 g/molN-(6-Hydroxyhexyl)trifluoroacetamide
CAS:Please enquire for more information about N-(6-Hydroxyhexyl)trifluoroacetamide including the price, delivery time and more detailed product information at the technical inquiry form on this pageFormula:C8H14F3NO2Molecular weight:213.2 g/mol2-Fluoro-4-nitrophenyl b-D-galactopyranoside
CAS:2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.Purity:Min. 95%Color and Shape:PowderMolecular weight:319.24 g/mol4-Methylumbelliferone
CAS:4-Methylumbelliferone is a coumarin derivative that inhibits the activity of enzymes such as p-nitrophenyl phosphate oxidase and aminopeptidase. It is used in vitro to study oxidative injury, which plays a role in the pathogenesis of inflammatory diseases, including primary sclerosing cholangitis and bone marrow transplantation. 4-Methylumbelliferone has been shown to induce apoptosis by increasing reactive oxygen species levels and activating toll-like receptor 4 (TLR4). It also has inhibitory effects on osteosarcoma cell proliferation in vitro.Formula:C10H8O3Molecular weight:176.17 g/mol4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-β-D-glucopyranoside
4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.Formula:C24H26O11SPurity:Min. 95%Color and Shape:White to off-white solid.Molecular weight:522.52 g/mol3-Nitrophenyl b-D-galactopyranoside
CAS:Chromogenic substrate targeting beta-galactosidaseFormula:C12H15NO8Color and Shape:PowderMolecular weight:301.25 g/mol4-Nitrophenyl-beta-D-lactopyranoside
CAS:Chromogenic substrate for cellobiohydrolase and endoglucanase.Formula:C18H25NO13Purity:Min. 99.0 Area-%Molecular weight:463.40 g/molAquaSpark® 515 Singlet Oxygen Probe, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191
CAS:Singlet Oxygen Chemiluminescence Probe, SOCLFormula:C21H23ClO4Purity:Min. 95 Area-%Color and Shape:PowderMolecular weight:374.13 g/mol4-Methylumbelliferyl N,N',N''-triacetyl-b-D-chitotrioside
CAS:4-Methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotrioside is a fluorogenic substrate for chitinase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl N,N',N''-triacetyl-beta-D-chitotriosideas a substrate for measuring the chitinase activity is used for fungal infection assays.Formula:C34H47N3O18Purity:Min. 95.0 Area-%Color and Shape:White Off-White PowderMolecular weight:785.75 g/mol6-Bromo-2-naphthyl b-D-glucopyranoside
CAS:6-Bromo-2-naphthyl b-D-glucopyranoside is an enzyme substrate used in chromogenic assays with beta-glucosidases. It has been used in culture media for the identification of Legionella pneumophila.Formula:C16H17BrO6Purity:Min. 98 Area-%Color and Shape:PowderMolecular weight:385.21 g/mol
