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Enzyme Substrates

Enzyme Substrates

Enzyme substrates are molecules upon which enzymes act to catalyze chemical reactions. The substrate binds to the active site of the enzyme, where it undergoes a specific transformation to form the product of the reaction. Enzyme substrates are essential tools in research for studying enzyme kinetics, specificity, and mechanism of action. They are also used in diagnostic assays and biochemical studies. At CymitQuimica, we provide a diverse selection of high-quality enzyme substrates to support your research in enzymology and biochemistry.

Subcategories of "Enzyme Substrates"

Found 1527 products of "Enzyme Substrates"

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  • 4-Nitrophenyl a-D-mannopyranoside

    CAS:

    4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production.

    Formula:C12H15NO8
    Purity:Min. 98.0 Area-%
    Color and Shape:Off-White Powder
    Molecular weight:301.25 g/mol

    Ref: 3D-EN03183

    5g
    501.00€
    10g
    769.00€
    25g
    1,633.00€
    50g
    2,904.00€
    100g
    5,256.00€
  • 8-Hydroxyquinoline-beta-D-glucuronic acid, sodium salt

    CAS:

    8-Hydroxyquinoline-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used for enzymatic detection of peroxidase. The substrate is available in a variety of forms, including powder and liquid. This product has been tested for environmental and bioluminescence activity, as well as food safety. It has also been shown to be a useful ligand in immunoassays.

    Formula:C15H14NNaO7
    Purity:Min. 98 Area-%
    Color and Shape:White Powder
    Molecular weight:343.26 g/mol

    Ref: 3D-EH05119

    1g
    303.00€
    5g
    735.00€
    10g
    1,149.00€
    25g
    2,234.00€
    2500mg
    455.00€
  • 4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxy-b-D-galactopyranoside

    CAS:

    4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.

    Formula:C26H38N2O17
    Purity:Min. 95%
    Molecular weight:650.58 g/mol

    Ref: 3D-EN45632

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  • AquaSpark® β-D-galactoside, lyophilized, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191


    Chemiluminescence beta-galactosidase substrate
    Formula:C34H39ClO12
    Molecular weight:675.12 g/mol

    Ref: 3D-A-8168_P00

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  • 4-Nitrophenyl a-L-arabinopyranoside

    CAS:
    Substrate for a-L-arabinopyranosidase
    Formula:C11H13NO7
    Purity:Min. 98 Area-%
    Color and Shape:White Powder
    Molecular weight:271.22 g/mol

    Ref: 3D-EN06879

    1g
    400.00€
    2g
    504.00€
    5g
    814.00€
    10g
    1,322.00€
    25g
    2,833.00€
  • 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside


    5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).
    Formula:C14H15BrClNO6
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:408.63 g/mol

    Ref: 3D-EB161470

    5mg
    136.00€
    10mg
    166.00€
    25mg
    296.00€
    50mg
    445.00€
  • 6-Chloro-3-indoxyl-beta-D-galactopyranoside

    CAS:
    6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.
    Formula:C14H16ClNO6
    Purity:Min. 98.0 Area-%
    Molecular weight:329.74 g/mol

    Ref: 3D-C-5000

    1g
    542.00€
    2g
    821.00€
    5g
    1,451.00€
    10g
    1,934.00€
    25g
    3,531.00€
  • L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride

    CAS:
    L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.
    Purity:Min. 98.0 Area-%
    Color and Shape:Powder

    Ref: 3D-EL172292

    1g
    1,551.00€
    5g
    5,710.00€
    250mg
    574.00€
    500mg
    946.00€
    2500mg
    3,284.00€
  • 5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside

    CAS:
    5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.
    Formula:C14H15BrClNO6
    Color and Shape:White to off-white solid.
    Molecular weight:408.63 g/mol

    Ref: 3D-EB03261

    1g
    641.00€
    5g
    1,988.00€
    10g
    3,227.00€
    25g
    6,721.00€
    2500mg
    1,193.00€
  • Resorufin b-D-xylobioside


    Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:477.42 g/mol

    Ref: 3D-ER76453

    1mg
    231.00€
    2mg
    411.00€
    5mg
    819.00€
    10mg
    1,322.00€
  • 2-Chloro-4-nitrophenyl α-D-mannopyranoside

    CAS:

    2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.

    Formula:C12H14ClNO8
    Purity:Min. 98 Area-%
    Color and Shape:White Powder
    Molecular weight:335.69 g/mol

    Ref: 3D-EC15457

    25mg
    338.00€
    50mg
    470.00€
    100mg
    602.00€
    250mg
    889.00€
    500mg
    1,259.00€
  • 4-Nitrophenyl b-L-arabinofuranoside

    CAS:

    Chromogenic substrate targeting alpha-L-arabinofuranosidase

    Purity:Min. 95%
    Color and Shape:White/Off-White Solid
    Molecular weight:271.22 g/mol

    Ref: 3D-EN31820

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  • 4-Methylumbelliferyl 2-acetamido-2-deoxy-α-D-glucopyranoside-6-sulfate potassium

    CAS:
    4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassium is a fluorogenic substrate for N-acetylglucosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassiumas a substrate for measuring the N-acetylglucosamine-6-sulfatase activity is used for screening of Mucopolysaccharidosis IIID (MPS IIID) (a rare lysosomal storage disorder) and Sanfilippo D syndrome diagnosis.
    Formula:C18H20KNO11S
    Purity:Min. 95%
    Color and Shape:White Powder
    Molecular weight:497.52 g/mol

    Ref: 3D-EM04850

    1mg
    200.00€
    2mg
    319.00€
    5mg
    617.00€
    10mg
    1,014.00€
    500µg
    138.00€
  • Resorufin-β-D-glucuronic acid, sodium salt

    CAS:
    Resorufin-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used in diagnostic applications. It is a conjugate of resorufin and beta-D-glucuronic acid. The enzyme substrate reacts with the enzyme luciferase to produce light. The high purity and quality of this product make it ideal for use in the detection of the presence of glucose in urine, which can be used to diagnose diabetes mellitus.
    Formula:C18H14NNaO9
    Purity:Min. 90.0 Area-%
    Molecular weight:411.30 g/mol

    Ref: 3D-R-2150

    2mg
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    5mg
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    10mg
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    25mg
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    50mg
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  • 4-Nitrophenyl-α-D-maltopyranoside

    CAS:
    Chromogenic α-maltosidase substrate yielding a yellow solution upon cleavage.
    Formula:C18H25NO13
    Purity:Min. 98.0 Area-%
    Molecular weight:463.40 g/mol

    Ref: 3D-N-4490

    1g
    538.00€
    2g
    892.00€
    5g
    1,802.00€
    10g
    2,925.00€
    500mg
    378.00€
  • Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride

    CAS:

    Glutaryl-glycyl-L-arginine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.

    Formula:C23H30N6O7·HCl
    Purity:Min. 95%
    Color and Shape:Powder
    Molecular weight:538.98 g/mol

    Ref: 3D-EG138823

    1g
    1,192.00€
    100mg
    302.00€
    250mg
    500.00€
    500mg
    742.00€
  • 4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside-6-sulfate


    4-Nitrophenyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chemiluminescent substrate that can be used in the detection of various enzymes. This product has been tested for sterility and purity, and it has passed all tests. It is also free of any detectable levels of heavy metals or other contaminants. 4NP2AG6S is a high quality bioluminescent substrate with CAS No. 71498-92-5. It is a fluorescent compound that reacts with an enzyme to produce light. The reaction can be detected by measuring the emitted light intensity at 680 nm (max).
    Purity:Min. 95%

    Ref: 3D-EN43871

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  • Aldol® 584 β-D-glucuronic acid, triethylammonium salt, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.
    Formula:C37H46ClN3O10
    Purity:Min. 95.0 Area-%
    Molecular weight:728.20 g/mol

    Ref: 3D-A-4768_P00

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  • N-α-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride

    CAS:
    N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a high quality and highly purified substrate for enzymes that catalyze the release of light when they react with their specific substrates. It is used in a variety of applications, including diagnostics and food testing. NACBZLAMC hydrochloride is an excellent ligand for chemiluminescence reactions and can be used to detect the presence of various analytes. It can also be used as a staining agent to detect proteins on Western blots or to stain bacterial cultures for identification. This product has been shown to be effective against numerous bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa, as well as fungi like Candida albicans.
    Formula:C24H28ClN5O5
    Purity:Min. 99 Area-%
    Molecular weight:501.97 g/mol

    Ref: 3D-C-2100

    1g
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    5g
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    10g
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    500mg
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    2500mg
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    -Unit-gg
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  • Ac-Abu-Tle-Leu-Gln-AMC TFA salt


    A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).
    Formula:C33H48N6O8(freebase)
    Purity:Min. 95.0 Area-%
    Molecular weight:656.77 g/mol

    Ref: 3D-FA178674

    1mg
    249.00€
    2mg
    371.00€
    5mg
    575.00€
    10mg
    853.00€
    25mg
    1,313.00€