Enzyme Substrates

2-Nitrophenyl b-D-arabinopyranoside is a chemical compound that is used in the detection of ATP by chemiluminescence. It acts as a substrate for enzymes such as luciferase and catalase, which catalyze the oxidation of 2-nitrophenyl b-D-arabinopyranoside to phenol and carbon dioxide with the release of light. This reaction can be used in diagnostic applications such as detecting bacteria in culture media or diagnosing food spoilage. 2-Nitrophenyl b-D-arabinopyranoside can also be used as a conjugate to detect proteins or nucleic acids by fluorescence spectroscopy. The CAS number for this compound is 107772-06-1.

Inorganic phosphate targeted colorimetric probe

Ostazin Brilliant Red H-3B Galactomannan is a chemiluminescent substrate used for the detection of β-1,4-galactomannans. It reacts with the glucose residues on the mannan backbone to produce a red fluorescent product. This product can be detected by spectrophotometry at an excitation wavelength of 395 nm and emission wavelength of 510 nm. The Ostazin Brilliant Red H-3B Galactomannan substrate is useful in detecting Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Group A streptococci, Group B streptococci, Group D streptococci, Group G streptococci, and Group C streptococci.

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium salt is a fluorogenic substrate for beta-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium saltas a substrate for measuring the beta-N-acetylglucosaminidase activity is used for diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB) (a rare lysosomal storage disorder) and Sanfilippo B syndrome screening.

5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).

BIOSYNTH Culture Medium for the isolation andidentification of Escherichia coli O157:H7

This botanical is a download for the Floral Database.

Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.