Enzyme Substrates

<p>5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.</p>

<p>Esculetin-7-beta-D-galactopyranoside, also known as 6,7-Dihydroxycoumaryl-7-beta-D-galactopyranoside, is a fluorogenic substrate used in the detection of beta-galactosidase activity. This coumarin derivative, when hydrolyzed, yields esculetin (6,7-dihydroxycoumarin) which, in the presence of iron salts, forms a brown-black complex.</p>

<p>6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.</p>

<p>Aldol 458 nonanoate is a high quality, chromogenic and fluorogenic substrate for use in enzymatic, chemiluminescent, and immunochemical reactions. Aldol 458 nonanoate is used as a chromogenic substrate for the detection of glucose oxidase activity in culture media and as a fluorogenic substrate for the detection of glucose oxidase activity in environmental testing. This product has CAS number 2484872-52-6.</p>

<p>Resorufin b-D-cellobioside is a fluorogenic substrate for measurement of cellulase activity. The product of enzyme reaction, resorufin, exhibits fluorescence emission at 585 nm with excitation at 571 nm at or near physiological pH values.</p>

<p>6-Chloro-4-(trifluoromethyl)umbelliferyl b-D-cellotrioside is a fluorogenic substrate for cellulases and beta-glucosidases. After hydrolisis, the fluorophore TFMU is released generating a blue fluorescence.</p>

<p>4-Methylumbelliferyl beta-D-apiofuranoside is a fluorescent substrate that is used in the detection of enzyme activity. It is a yellow crystalline powder with a melting point of 132°C and an optical rotation of +27°. 4-Methylumbelliferyl beta-D-apiofuranoside can be used as a conjugate for fluorescence, chemiluminescence, or bioluminescence detection of enzymes such as β-galactosidase, phosphatases, esterases, and peroxidases. It is also used to stain cells and tissues for microscopic analysis. This product has been tested for its effects on the environment and found to have little potential for harm to the environment. 4-Methylumbelliferyl beta-D-apiofuranoside has been shown to be high purity with no significant impurities detected in testing. The chemical has shown high quality characteristics in</p>

<p>N-Methylindoxyl-beta-D-galactopyranoside monohydrate (MG) is a chromogenic probe that interacts with galactose residues found on glycoproteins, which are found on cell surfaces, leading to activation of cells. MG also binds specifically to the surface of lung cells and can inhibit the development of certain types of infections..</p>

<p>5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is a chromogenic substrate used to detect the specific enzymatic activity of alkaline phosphatase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is used in ELISA and molecular biology assays such as lacZ reporter systems.</p>

<p>2-Naphthyl butyrate is used as a chromogenic substrate in hematological studies via measuring esterase activity. This type of assays are important as it can help in distinguishing acute leukemias with monocytic or granulocytic differentiation. After hydrolysis of 2-naphthyl butyrate by the esterase (for example acylase), b-napthol is released that can be quantified spectrophotometrically (Exc 320 nm; Em 410 nm).</p>

<p>4-Nitrophenyl sulfate potassium salt is a chromogenic substrate for arylsulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl sulfate potassium salt is used in different applications such as the Metachromatic leukodystrophy diagnosis, sulfatase activity assays</p>

<p>Please enquire for more information about BCM® Staphylococcus aureus Plating medium including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chromogenic substrate for beta-glucuronidase</p>

<p>4-Methylumbelliferyl-alpha-L-rhamnopyranoside is a fluorogenic substrate used in enzyme assays. It is a substrate of the enzyme beta-galactosidase, which catalyzes the hydrolysis of terminal galactose residues from polysaccharides and glycoproteins. The fluorescent product 4-methylumbelliferone (4MU) is formed by cleavage of the terminal galactose residue and can be measured spectrophotometrically at 360 nm. 4-Methylumbelliferone has been used for chemiluminescent detection in immunoassays and as a substrate for bioluminescence reactions.</p>

<p>Aldol® 470 butyrate, a chromogenic substrate manufactured by Cymit Quimica and used for enzymatic assays that produces a yellow-orange color upon enzymatic cleavage.</p>

<p>A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.</p>

<p>3-(4,4-Difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionamido)propyl b -D -lactoside (FDPBP) is a substrate for alkaline phosphatase. It reacts with the enzyme to produce a blue color. FDPBP is used in diagnostic applications such as food testing and bioluminescence.</p>

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. This may also be used for in-gel assays</p>

<p>Caspases are a family of protease enzymes playing essential roles in programmed cell death and inflammation. The acronym caspase stands for Cysteine Aspartic Acid Specific Protease due to their specific cleavage of an aspartic acid residue, by mean of a cysteine in the catalytic site of the enzyme._x000D_About 11 or 12 different caspases are known in humans and 10 in mice, participating in numerous different cellular functions. Caspase deficiency, for example, has been identified as a cause of tumor development, while over-activation of some caspases such as caspase-3 can lead to excessive apoptosis that can be observed in several neurodegenerative diseases. Caspases cleave proteins at specific peptide recognition sequences. Ac-DEVD-6-Amino-D-Luciferin (Ac-Asp-Glu-Val-Asp-6-Amino-D-Luciferin, Cat.No_x000D_L-8282) is a synthetic chemiluminescent peptide for the determination of caspase-3 and caspase-7activity.</p>

<p>Luc-S.aureus K is a fluoro-ligand that is used as a diagnostic to detect and quantify the activity of S.aureus in any given sample. It is a detection substrate for the enzyme β-galactosidase, which catalyzes the hydrolysis of the luciferin. When luciferin is cleaved by β-galactosidase, it emits light that can be detected using a luminometer to measure the amount of light produced and calculate the quantity of bacteria present in the sample. Luc-S.aureus K has been approved for use as a detector for bioluminescence in food testing, environmental testing, and other diagnostic applications.</p>