Chromogenic Substrates

<p>2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.</p>

<p>Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.</p>

<p>6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.</p>

<p>4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics</p>

<p>4-Nitrophenyl sulfate potassium salt is a chromogenic substrate for arylsulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl sulfate potassium salt is used in different applications such as the Metachromatic leukodystrophy diagnosis, sulfatase activity assays</p>

<p>5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid ammonium salt is a substrate used to detect and quantify sialidase activity. Sialidases are enzymes that cleave sialic acid residues from glycoconjugates, and their activity is important in various cellular processes. The substrate is commonly used in research aimed at understanding the role of sialidases in diseases such as influenza and cancer.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>5-Bromo-4-chloro-3-indoxyl oleate is a chromogenic substrate used to detect the specific enzymatic activity of lipase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl oleate is used in lipid metabolism disorder screening.</p>

<p>Beta-naphthyl stearate is a chromogenic substrate for measuring enzymatic activity.</p>

<p>5-Bromo-3-indolyl phosphate disodium salt is a chemiluminescent substrate that is used for the detection of bacterial activity. It reacts with oxygen to produce light, which can be measured using a luminometer. It is also used as a chromogenic substrate for detection of bacterial activity in culture media. The enzyme reaction produces an intense blue fluorescence and is used for diagnostic purposes in food testing and medical diagnostics. 5-Bromo-3-indolyl phosphate disodium salt has been shown to be effective against many bacteria and fungi, including Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. This product is chemically stable at room temperature but should be stored at 4°C or below.</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.</p>

<p>Nalpha-CBZ-L-Citrulline-p-nitroanilide is a synthetic chromogenic enzyme substrate used in protease enzymatic stutides. After hydrolysis, p-nitroaniline is released, which produces a yellow color detectable spectrophotometrically at 405 nm.</p>

<p>Aldol® 470 nonanoate is a chemiluminescent substrate that can be used as a ligand for the detection of C8/C9-esterases, lipases activity, to result in an orange/green color. Aldol® 470 nonanoate has been shown to be active with horseradish peroxidase in a number of different assay systems. The purity and quality of Aldol® 470 nonanoate has been tested with culture media, environmental testing, bioluminescence, staining, fluorogenic substrate, chromogenic substrate, enzyme substrate, food testing and conjugates.</p>

<p>6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.</p>

<p>Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.</p>

<p>4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.</p>

<p>Chromogenic substrate for alpha-L-fucosidase</p>

<p>4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose degradation studies, biofuel research</p>

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>