Chromogenic Substrates
Chromogenic substrates are compounds that produce a visible color change when acted upon by specific enzymes. These substrates are widely used in diagnostic assays, biochemical research, and enzyme-linked immunosorbent assays (ELISAs) to detect and quantify enzyme activity. The color change allows for easy and rapid visual identification of enzyme reactions, making chromogenic substrates essential tools in laboratory analysis. At CymitQuimica, we provide a diverse selection of high-quality chromogenic substrates to support your research and diagnostic applications.
Found 712 products of "Chromogenic Substrates"
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2-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)]-2-deoxy-b-D-glucopyranoside
2-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)]--2-deoxy--bDglucopyranoside is an acylated glucopyranoside that is used as a substrate for the enzyme β -galactosidase. When hydrolyzed by β -galactosidase, it yields a blue product that has been widely used in chemiluminescence and bioluminescence assays. It also can be used for staining and ligand binding studies.
Purity:Min. 95%Molecular weight:884.83 g/mol4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-O-benzylidene-b-D-glucopyranoside
CAS:4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-O-benzylidene-b-D-glucopyranoside is a compound that is used in gene expression profiling. It has been shown to be an effective inhibitor of glutamicum and corynebacterium glutamicum. 4NPBG can inhibit the production of acid molecules by blocking the activity of cytochrome P450 enzymes in the cells of these bacteria. The compound also inhibits cellular transport and membrane transport genes in these species. This inhibition prevents the synthesis of nucleic acid molecules such as RNA and DNA.Purity:Min. 95%Molecular weight:719.64 g/mol4-Nitrophenyl β-D-glucuronide sodium
CAS:4-Nitrophenyl b-D-glucuronide sodium salt is a high-quality chromogenic substrate specifically designed for the colorimetric detection and measurement of β-glucuronidase activity. Upon enzymatic cleavage, it releases the yellow 4-nitrophenol, enabling quantitative analysis via spectroscopy. This compound is conveniently provided as a sodium salt to ensure enhanced solubility, stability, and shelf life. It is an ideal choice for biochemical, research, and diagnostic applications requiring a reliable and efficient b-D-glucuronide substrate.Formula:C12H12NNaO9Purity:Min. 95%Molecular weight:337.21 g/mol1-Naphthyl a-D-glucopyranoside
CAS:1-Naphthyl a-D-glucopyranoside is a chromogenic substrate used to detect the presence of enzymes that catalyze the hydrolysis of glycosidic bonds, such as alpha-glucosidase. When the substrate is cleaved by the enzyme, it produces a colored product that can be visualized and quantified.Purity:Min. 95%Molecular weight:306.31 g/mol5-Bromo-3-indolyl decanoate
CAS:5-Bromo-3-indolyl decanoate (BID) is a chromogenic enzyme substrate often used for the detection of lipase activity. It is hydrolyzed by lipase to produce a blue-violet product, which can be detected visually or by spectrophotometry. BID is particularly useful for the detection of microbial lipases in food and environmental samples. It is commonly used in a wide range of biochemical, biological, and environmental testing applications that require precise lipase enzyme analysis.Formula:C18H24BrNO2Purity:Min. 95%Color and Shape:PowderMolecular weight:366.29 g/mol4-Nitrophenyl hepta-O-acetyl-1-thio-b-lactoside
CAS:4-Nitrophenyl hepta-O-acetyl-1-thio-b-lactoside is an inorganic molecule that binds to the surface of bacteria. It has been used as a fingerprinting tool for a variety of organisms, including corynebacterium glutamicum. The molecule can be used to measure the metabolic activity of corynebacterium glutamicum with a technique called incremental acidification. This technique is based on the observation that the concentration of 4-nitrophenol decreases by one unit for each glucose metabolized by corynebacterium glutamicum. The use of 4NPHTBL and other primers can also be useful in diagnosing patients with autoimmune diseases such as celiac disease and inflammatory bowel disease.Purity:Min. 95%4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside
CAS:4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside is a fluorescent substrate for beta-galactosidase that is used in diagnostics and research. The product has been shown to be an excellent substrate for beta-galactosidase and beta-glucuronidase, as well as other glycosidases. It can be used to detect the presence of these enzymes in any sample containing glycosides. This product is also used in food testing, environmental testing, and bioluminescence experiments. 4NPAFG is available in high purity with a conjugate group at the C4 position.Purity:Min. 95%Molecular weight:488.44 g/mol4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)-α-D-galactopyranoside
CAS:4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)-α-D-galactopyranoside is a chemiluminescent substrate that has been used in the detection of bacterial activity. It is used in the detection of bacterial activity through chemiluminescence and can be conjugated to an enzyme or protein for increased sensitivity. This product has been shown to have high purity and quality and is suitable for use in diagnostic applications.Formula:C28H36N2O17Purity:Min. 95%Color and Shape:PowderMolecular weight:672.59 g/molEspresso-Gal
Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.Molecular weight:495.6 g/mol4-Nitrophenyl 6-O-trityl-a-D-glucopyranoside
CAS:4-Nitrophenyl 6-O-trityl-a-D-glucopyranoside is a high-quality trityl pNP substrate that performs excellently in various enzymatic assays. Containing a trityl-protected sugar moiety, this substrate is perfect for investigating alpha-D-glucopyranoside-specific enzymes and providing precise data for your experiments.Purity:Min. 95%Molecular weight:543.56 g/mol2-Nitrophenyl caprylate
CAS:2-Nitrophenyl caprylate is a potent inhibitor of gram-negative bacteria. It has been shown to inhibit the enzymatic activity of certain bacterial enzymes, such as the modification of cellular fatty acids. 2-Nitrophenyl caprylate has also been shown to inhibit in vitro growth of various gram-negative bacteria, including E. coli and Salmonella enterica serovar Typhimurium, through detergent compositions and enzymatic reactions (e.g., aminopeptidases). 2-Nitrophenyl caprylate may be useful for the treatment of pancreatitis due to its inhibition of pancreatic secretions and inhibition of pancreatic lipase activity. Transposable sequences have been found in the 2-nitrophenyl caprylate gene cluster, which may be related to its regulatory properties.Purity:Min. 95%Color and Shape:PowderMolecular weight:265.31 g/mol4-Nitrophenyl hepta-O-acetyl-b-lactoside
CAS:4-Nitrophenyl hepta-O-acetyl-b-lactoside is a high-quality chromogenic pNP enzyme substrate that exhibits excellent sensitivity and specificity in enzyme assays. This synthetic compound is designed to react with β-galactosidase enzymes, generating a yellow product (4-nitrophenol) upon cleavage. The resulting color change allows for quantitative measurement of enzyme activity, providing valuable insights in various research applications including enzyme kinetics, screening of inhibitors, drug discovery, and molecular diagnostics. Our product ensures consistent results with minimal interference, making it a reliable choice for a wide range of biochemical applications.Purity:Min. 95%Molecular weight:757.65 g/molBCIP disodium salt sesquihydrate
CAS:BCIP is a dye that can be used as a substrate in a fluorometric assay to detect the presence of effector proteins. It is also used in analytical chemistry to detect phosphate, and as a reagent in diagnostic tests for mucin gene expression. BCIP has been shown to have transcriptional regulatory effects on genes encoding for proteins involved in antibody production. BCIP has been shown to act as an effector protein, which influences the amount of mRNA produced by the gene encoding for tissue culture cells. BCIP can also be used as a model system to study the regulation of transcription in vitro.
A hybridization technique using BCIP with DNA-dependent RNA polymerase II has been demonstrated and applied to analyze the mechanism of transcriptional control at the level of individual genes. The use of this technique allows for analysis at a single gene level and may allow for better understanding of transcriptional regulation.Purity:Min. 95%Molecular weight:388.45 g/mol2-Nitrophenyl 6-O-trityl-b-D-galactopyranoside
CAS:2-Nitrophenyl 6-O-trityl-b-D-galactopyranoside is a chromogenic enzyme substrate used in assays to detect galactosidase activity. It is a derivative of galactose that can be hydrolyzed by galactosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. The trityl group helps to stabilize the molecule and prevent undesired hydrolysis during storage. This substrate is commonly used in the study of carbohydrate metabolism and in the detection of galactosidase activity in microorganisms.Purity:Min. 95%Molecular weight:543.56 g/mol4-Nitrophenyl 2-O-(α-L-fucopyranosyl)-β-D-galactopyranoside
CAS:4-Nitrophenyl 2-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is a chromogenic enzymatic substrate for the detection and quantification of glycosidases, such as fucosidases and galactosidases. Upon enzymatic cleavage, the substrate releases the p-nitrophenol chromophore, which can be quantified via colorimetric analysis. This pNP-conjugated compound enables the rapid, sensitive, and high-throughput detection of enzyme activity in various applications, including enzymatic assays, inhibitor screening, and mechanistic studies. Its precise specificity for α-L-fucopyranosyl and β-D-galactopyranoside moieties makes it an excellent tool for advancing research in glycobiology and related fields.Formula:C18H25NO12Purity:Min. 95%Color and Shape:White Off-White PowderMolecular weight:447.39 g/mol4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-b-D-glucopyranoside
CAS:4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-b-D-glucopyranoside is a fluorescent substrate that can be used for the detection of β-galactosidase. It fluoresces when cleaved by β-galactosidase, which is an enzyme that catalyzes the hydrolysis of terminal galactose residues in the glycosidic linkage of β-galactosides. This product is an excellent substrate for use in chemiluminescence and bioluminescence assays. 4NPBG has been used to detect β-galactosidase activity in diverse environments, such as food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, and conjugate. It is also used in diagnostics, ligand binding assays,Purity:Min. 95%Molecular weight:754.64 g/mol2-(5'-Chloro-2'-hydroxyphenyl)-6-chloro-4(3H)-quinazolinonyl a-D-N-acetylgalactosamine
2-(5'-Chloro-2'-hydroxyphenyl)-6-chloro-4(3H)-quinazolinonyl a-D-N-acetylgalactosamine is an inhibitor of bacterial cell wall synthesis. It has been shown to inhibit the enzymatic activity of beta-lactamase, which is an enzyme that breaks down beta-lactam antibiotics. 2-(5'-Chloro-2'-hydroxyphenyl)-6-chloro-4(3H)-quinazolinonyl a-D-N-acetylgalactosamine can be used for the treatment of infections caused by bacteria such as methicillin resistant Staphylococcus aureus. This compound has been shown to have low toxicity and does not cause any significant side effects in animals.Purity:Min. 95%Molecular weight:510.32 g/mol5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid methyl ester
CAS:5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid methyl ester is a chromogenic substrate that reacts with enzymes to produce a colored product. It can be used in diagnostic tests for the detection of various bacteria, including Salmonella and Brucella, as well as for the determination of concentration in food testing or environmental testing. 5B4C3INA can also be conjugated to other substances, such as biotin or an antibody, which allows it to be used in immunoassays.Formula:C20H24BrClN2O9Purity:Min. 95%Molecular weight:551.77 g/mol4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-b-L-fucopyranosyl)-6-O-benzoyl-a-D-galactopyranoside
CAS:4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-b-L-fucopyranosyl)-6-O-benzoyl-a-D-galactopyranoside is a chemiluminescent substrate with a CAS number of 104341548. It is used as a fluorescent probe for the detection of alpha amylase, beta amylase and glucose oxidase. It can be used to detect bacteria in food. This substrate can be used in the diagnostic industry and is an important component for the production of various enzymes.Purity:Min. 95%Molecular weight:677.61 g/mol4-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester
CAS:4-Nitrophenyl 2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a fluorescent substrate for the beta-glucuronidase enzyme. It can be used to detect the presence of this enzyme in bacteria and other microorganisms by means of fluorometric or colorimetric assays. It has been shown to have an excellent stability in a variety of culture media and food testing applications. This product is available in high purity and quality, with a CAS number of 18472-49-6.Purity:Min. 95%Molecular weight:455.37 g/mol
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