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Chromogenic Substrates

Chromogenic Substrates

Chromogenic substrates are compounds that produce a visible color change when acted upon by specific enzymes. These substrates are widely used in diagnostic assays, biochemical research, and enzyme-linked immunosorbent assays (ELISAs) to detect and quantify enzyme activity. The color change allows for easy and rapid visual identification of enzyme reactions, making chromogenic substrates essential tools in laboratory analysis. At CymitQuimica, we provide a diverse selection of high-quality chromogenic substrates to support your research and diagnostic applications.

Found 712 products of "Chromogenic Substrates"

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  • 2-Nitrophenyl 2,3-di-O-acetyl-b-D-xylopyranoside

    CAS:

    2-Nitrophenyl 2,3-di-O-acetyl-b-D-xylopyranoside is a chromogenic substrate used to assay the activity of xylosidase enzymes. It is hydrolyzed by xylosidase to yield a yellow-colored product, 2-nitrophenol, which absorbs light at 405 and 410 nm. This substrate is used in research to study the function of xylosidase enzymes and their role in various biological processes. It is also used in diagnostic tests for the detection of xylosidase activity in different microorganisms, including bacteria and fungi.

    Purity:Min. 95%
    Molecular weight:355.3 g/mol

    Ref: 3D-EN06455

    2mg
    305.00€
    5mg
    367.00€
    10mg
    572.00€
  • 4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside

    CAS:

    4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.

    Purity:Min. 95%
    Molecular weight:650.58 g/mol

    Ref: 3D-EN45633

    1mg
    349.00€
    5mg
    1,180.00€
    10mg
    1,887.00€
  • 2,4-Dinitrophenyl b-D-cellobioside

    CAS:
    2,4-Dinitrophenyl b-D-cellobioside is a chemiluminescent substrate that is used to detect the activity of enzymes and other proteins. It can be conjugated with an antibody or other ligand for use in diagnostic purposes. 2,4-DNPBC is a pure compound with a high quality that does not contain any other impurities. Its CAS number is 157956-96-2.
    Purity:Min. 95%

    Ref: 3D-ED165936

    100mg
    2,555.00€
    250mg
    3,920.00€
    500mg
    5,003.00€
  • N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride

    CAS:

    N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride is a fluorogenic substrate that can be used for the detection of β-glucuronidase. It has been shown to have high purity and quality, which makes it an excellent choice for use in food testing and staining.

    Formula:C26H34N8O6•HCl
    Purity:Min. 95%
    Molecular weight:591.06 g/mol

    Ref: 3D-EB182653

    2mg
    305.00€
    5mg
    491.00€
    10mg
    700.00€
    25mg
    1,202.00€
    50mg
    1,802.00€
  • 2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside

    CAS:
    2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside is a chromogenic enzyme substrate used in the biochemical characterization of various enzymes. It produces a yellow color when cleaved by enzymes and is commonly used in assays to study enzyme kinetics and activity. It is commonly used is used to study the biochemical and physiological effects of glycosylation and acts as a substrate for glycosylation-dependent enzymes involved in carbohydrate metabolism. Glycosidases hydrolyse 2-Chloro-4-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranoside, releasing 2-chloro-4-nitrophenyl moiety, and the substrate can then be further metabolized by other enzymes. It is also used in the investigate the effects and activity on glycoproteins and glycolipids.
    Purity:Min. 95%
    Molecular weight:503.84 g/mol

    Ref: 3D-EC03194

    1g
    1,675.00€
    50mg
    305.00€
    100mg
    343.00€
    250mg
    615.00€
    500mg
    1,008.00€
  • 4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside

    CAS:
    4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene a D galactopyranoside is an analog of galactose that binds to the DNA and regulates gene expression. This compound has been shown to be able to regulate gene expression levels in cells. 4NP2B6OB was used as a probe for microarray analysis of gene expression profiles from environmental samples. It was found that 4NP2B6OB could be used as a probe for the identification of genes in high density arrays.
    Purity:Min. 95%
    Molecular weight:717.67 g/mol

    Ref: 3D-EN06603

    2mg
    305.00€
    5mg
    366.00€
    10mg
    505.00€
    25mg
    1,034.00€
  • 4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-3,4,6-tri-O-acetyl-b-D-glucopyranosyl)-4,6-di-O-acetyl-2-deoxy-a-D-galactopyranoside


    4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-3,4,6-tri-O-acetyl-b-D-glucopyranosyl)-4,6-di-O-acetyl-2deoxy--aDgalactopyranoside is a substrate for enzymes such as beta galactosidase and alpha amylase. It can be used for the detection of these enzymes in biological samples, including culture media and body fluids. 4NPAG has also been used as a chromogenic substrate to measure the activity of NADH oxidase in neutrophils. 4NPAG is fluorescent in aqueous solution and can be used to detect bioluminescence reactions.
    Purity:Min. 95%
    Molecular weight:755.68 g/mol

    Ref: 3D-EN06445

    1mg
    305.00€
    5mg
    349.00€
    10mg
    515.00€
  • 5-Bromo-4-chloro-1H-indol-3-yl-b-D-glucuronide

    CAS:
    5-Bromo-4-chloro-3-indolyl β-D-glucuronide (BCIG) is an chromogenic enzyme substrate used to detect the presence of glucuronidase enzyme activity. The reaction occurs when BCIG reacts with the enzyme beta-glucuronidase yielding an intense blue color. It is used in a variety of biochemical and biomedical applications, such as the detection of pathogenic bacteria, the diagnosis of genetic disorders, and the isolation of recombinant proteins.
    Formula:C14H13BrClNO7
    Purity:Min. 95%
    Molecular weight:422.61 g/mol

    Ref: 3D-EB03256

    1g
    2,838.00€
  • 2-Nitrophenyl 2,2',3,3',4'-penta-O-acetyl-b-D-xylobioside

    CAS:
    2-Nitrophenyl 2,2',3,3',4'-penta-O-acetyl-b-D-xylobioside is an oligosaccharide that is used as a substrate for the transport of nucleic acid molecules. It can be used to study the function of membrane transport proteins and their homologues. This compound has been shown to inhibit the growth of corynebacterium glutamicum and trisaccharide emissions.
    Purity:Min. 95%
    Molecular weight:613.52 g/mol

    Ref: 3D-EN06793

    1mg
    305.00€
    2mg
    308.00€
    5mg
    481.00€
  • 4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thiogalactopyranoside

    CAS:
    4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thiogalactopyranoside (NPG) is a fluorogenic substrate that can be used in the detection of beta-galactosidase activity. It reacts with 4-nitrophenol to form an orange product at pH 6.0. The fluorescence intensity increases with increasing concentrations of the substrate and enzyme. The reaction is inhibited by borax, which competes for the enzyme active site. This product has been used as a chromogenic substrate for beta-galactosidase and as an enzyme substrate for beta-galactosidase in diagnostic tests for galactosemia and other carbohydrate metabolism disorders. NPG has also been used in chemiluminescent assays to detect beta-galactosidase activity in culture media and as a bioluminescence substrate in the detection of bacterial
    Purity:Min. 95%
    Molecular weight:485.47 g/mol

    Ref: 3D-EN04939

    2g
    305.00€
    5g
    328.00€
    10g
    515.00€
  • 3-Indolyl b-D-cellotrioside


    3-Indolyl b-D-cellotrioside is a chemiluminescent substrate that is used for the detection of bacterial activity. 3-Indolyl b-D-cellotrioside reacts with hydrogen peroxide to produce light, which can be detected by luminometers. This product can be used in diagnostic tests to detect the presence of bacteria in food, water, and other samples. It has been shown to have excellent performance characteristics and high purity and quality.
    Purity:Min. 95%
    Molecular weight:619.57 g/mol

    Ref: 3D-EI45056

    5mg
    1,748.00€
    10mg
    2,412.00€
  • Aldol® 470 N-tosyl-L-alanine ester, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:

    Aldol® 470 N-tosyl-L-alanine ester is a fluorogenic substrate that is used in enzyme and chromogenic assays. It is a high purity, high quality, and highly soluble substrate for chemiluminescent reactions. Aldol® 470 N-tosyl-L-alanine ester can be used to detect the presence of bacteria in culture media and as an indicator of bacterial growth. It is also used to test for the presence of different types of enzymes that catalyze the oxidation of alcohols.

    Formula:C33H30N2O7S
    Purity:Min. 90.0 Area-%
    Molecular weight:598.67 g/mol

    Ref: 3D-A-4712_P00

    1g
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  • Aldol® 467 β-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:

    Aldol® 467 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 467 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 467 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

    Formula:C29H28ClNO9
    Purity:Min. 95.0 Area-%
    Molecular weight:570 g/mol

    Ref: 3D-A-4676_P00

    1g
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  • Aldol® 544 β-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol 544 beta-D-galactopyranoside is a chromogenic substrate for the detection of beta-galactosidase activity in food testing and diagnostics. Aldol 544 beta-D-galactopyranoside is used as a conjugate to detect the presence of an enzyme, such as beta-galactosidase, in biological samples. It has been used in environmental testing and as a fluorogenic substrate for the detection of β-galactosidase activity.
    Formula:C33H33ClN2O7
    Purity:Min. 95.0 Area-%
    Molecular weight:605.08 g/mol

    Ref: 3D-A-4738_P00

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  • Aldol® 479 acetate, Biosynth Patent: EP 2427431 and US 8940909

    Controlled Product
    CAS:

    Aldol acetate is a chromogenic substrate that is used in food testing and diagnostics. Aldol acetate can be used in the detection of aminopeptidase and dipeptidyl peptidase activities, as well as in the determination of urinary proteins. Aldol acetate can also be used to detect anaerobic bacteria. This compound is conjugated with various ligands for use in immunoassays, such as beta-galactosidase, beta-glucuronidase, alpha-naphthyl phosphate ester, and phenyl phosphate ester. This product has been shown to be useful for chemiluminescent assays of DNA polymerases and reverse transcriptases in environmental samples. It has also been shown to be useful for bioluminescent assays of DNA polymerases and reverse transcriptases in environmental samples.

    Formula:C25H22N2O3
    Purity:Min. 95.0 Area-%
    Molecular weight:398.45 g/mol

    Ref: 3D-A-4729_P00

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  • 4-Nitrophenyl 2-O-(a-L-fucopyranosyl)-a-D-galactopyranoside

    CAS:
    4-Nitrophenyl 2-O-(a-L-fucopyranosyl)-a-D-galactopyranoside is a chromogenic pNP enzyme substrate consisting of a fucose and galactose moieties. Upon enzymatic hydrolysis by specific fucosidases or galactosidases, it releases the highly chromogenic molecule 4-nitrophenol, which exhibits a distinct yellow color that can be monitored spectrophotometrically. This substrate is frequently used to study enzyme specificity, kinetics, and inhibition, and it plays a critical role in the investigation of carbohydrate active enzymes (CAZymes), the screening of enzyme inhibitors, and the development of novel analytical methods for the detection and quantitation of enzyme activities.
    Purity:Min. 95%
    Molecular weight:447.39 g/mol

    Ref: 3D-EN06127

    1mg
    424.00€
    2mg
    719.00€
    5mg
    1,530.00€
    10mg
    2,384.00€
  • Aldol® 484 β-D-galactopyranoside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.
    Formula:C26H24ClFN2O7
    Purity:Min. 95.0 Area-%
    Molecular weight:530.93 g/mol

    Ref: 3D-A-4697_P00

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  • Phosphoric acid, mono(4-nitrophenyl) ester

    CAS:
    Phosphoric acid, mono(4-nitrophenyl) ester is an inhibitor that binds to the human serum albumin (HSA), thereby preventing it from binding to the transcriptional regulatory protein. This leads to a decrease in the activity of this protein and consequently less inhibition of gene expression. The structural analysis of this inhibitor has shown that it forms hydrogen bonding interactions with basic proteins, while its reactivity is mainly dependent on Michaelis–Menten kinetics. The inhibitor also inhibits enzyme activities by binding to their active sites, reducing the enzyme's ability to catalyze reactions.
    Formula:C6H6NO6P
    Molecular weight:219.09 g/mol

    Ref: 3D-P-6460

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  • Aldol® 518 β-D-cellobioside, Biosynth Patent: EP 2427431 and US 8940909

    CAS:
    Aldol® 518 beta-D-cellobioside is a chromogenic substrate for the detection of glycosidases. Aldol® 518 beta-D-cellobioside reacts with enzymes such as alpha-amylase, beta-galactosidase, and alpha-glucosidase to produce a fluorescent product. This product is detected by spectrophotometer, fluorometer, or luminometer. The fluorescence intensity is proportional to the concentration of the enzyme in the sample. It has been shown that Aldol® 518 beta-D-cellobioside can be used as a substitute for conventional substrates in food testing and diagnostics, such as staining and culture media. It also has applications in environmental testing and enzyme substrate assays.
    Formula:C35H39ClN2O12
    Purity:Min. 95 Area-%
    Molecular weight:715.15 g/mol

    Ref: 3D-A-4751_P00

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  • 6-Bromo-2-naphthyl-α-D-mannopyranoside

    CAS:

    6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a strong inhibitor of alpha-mannosidase. It has been shown to inhibit the growth of Mycobacterium tuberculosis and Streptococcus pneumoniae, which are both known to cause bacterial infections in humans. 6BNA inhibits the enzyme alpha-mannosidase, which hydrolyzes terminal α-(1,4)-linked mannose residues from oligosaccharides and glycoproteins. This drug has a high affinity for the active site of alpha-mannosidase and an IC50 value of 0.2 mM, making it an effective inhibitor.

    Formula:C16H17BrO6
    Purity:Min. 98.0 Area-%
    Molecular weight:385.22 g/mol

    Ref: 3D-B-8930

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