Chromogenic Substrates

<p>Chromogenic cephalosporin substrate for the detection of beta-lactamase activity</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-xylosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is used in bacterial xylan metabolism studies and plant cell wall degradation assays.</p>

<p>2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside, also known as Gal-G2-CNP, is a high quality, water soluble, chromogenic substrate that is used as an enzyme substrate and can be used in bioluminescence and chemiluminescence assays. This product is designed for use in diagnostics, culture media, food testing, environmental testing, and other research applications. 2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside has been tested in chromogenic assays with alpha-amylases.</p>

<p>6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.</p>

<p>Chromogenic substrate for esterase with C9 activity yielding a salmon colored precipitate upon cleavage.</p>

<p>5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).</p>

<p>Chromogenic substrate for α-glucanase</p>

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>

<p>4-Nitrophenyl beta-D-xylobioside is a chromogenic substrate for xylanase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-xylobioside is used in different applications such as the Xylan degradation studies, paper/pulp industry applications</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.</p>

<p>Chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. Used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.</p>

<p>Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.</p>

<p>Chromogenic sulfatase substrate</p>

<p>Substrate for horseradish peroxidase</p>

<p>For detection of beta-galactosidase activity. <br>The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.</p>

<p>Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate used to measure phospholipase C (PLC) activity</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Isopropyl-beta-D-thiogalactopyranoside (IPTG) is a non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression of lac operon to take place. IPTG is present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.</p>

<p>6-Bromo-2-naphthyl β-D-galactopyranoside is a chromogenic substrate commonly used for the detection of the enzymatic activity of β-galactosidase. It can produce a yellow precipitate upon hydrolysis by β-galactosidase, indicating the presence of the enzyme. It is often used in molecular biology applications to detect gene expression or to monitor cloning efficiency.</p>

<p>Please enquire for more information about 5-Nitro-3-indoxyl-β-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.</p>

<p>4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of α-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.</p>

<p>Chromogenic substrate for α-L-Fucosidase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis.</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-glycolylneuraminic acid is commonly used to measure the activity of sialidase enzymes, specifically those that cleave glycoconjugates containing 2-O-(4-nitrophenyl)-alpha-D-N-glycolylneuraminic acid. The resulting product can be detected by measuring the absorbance at a specific wavelength using a spectrophotometer. This substrate offers a high degree of specificity for glycolylneuraminic acid-containing substrates and is useful for both research and diagnostic purposes.</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases.</p>

<p>2-Naphthyl caprylate is a substrate that is employed in an assays for carboxylesterase and lipase and when hydrolyzed, produces 2-naphthol, a compound that can also be identified via fluorescence analysis. By combining it with a diazonium salt, the formation of the corresponding azo dye occurs. In particular, this substrate is used to detect the extracellular lipase of Pseudomonas fluorescens B52.</p>

<p>2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.</p>

<p>Chromogenic substrate for cellobiohydrolase and endoglucanase.</p>

<p>Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their beta-galactosidase activity.</p>

<p>Chromogenic substrate yielding a soluble chromophore for the colorimetric assay of α-D-galactosidase upon cleavage. Used for the detection of hydrolases in tissues and membranes.</p>

<p>Synthetic chromogenic substrate for detection of lipase enzyme activity that produces a red coloured product, Suitable for use in serum of plasma matrices. This lipase substrate is commonly used in veterinary diagnostics for monitoring and detecting canine pancreatitis,</p>

<p>Chromogenic substrate for alpha-L-rhamnosidase</p>

<p>5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.</p>

<p>6-Chloro-3-indoxyl-3-acetate is a chromogenic substrate for carboxylesterase yielding a salmon colored precipitate upon cleavage. Mainly used in histochemistry.</p>

<p>4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is a chromogenic substrate for glucose-6-phosphatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is used in different applications such as the Glycogen storage disease diagnosis (e.g., von Gierke disease)</p>

<p>Histochemical substrate for alkaline phosphatase</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>Aldol® 518 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase-producing bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 518 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>2-Nitrophenyl b-D-xylobioside is a chromogenic substrate for the enzyme xylanase.</p>

<p>4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.</p>

<p>2-Chloro-4-nitrophenyl b-D-maltoheptaoside is a fluorescent compound that is used in medical research for the visualization of muscle glycogen. It is also used to study the binding of molecules to proteins, as well as to measure enzyme activity. The compound has been shown to be insensitive to changes in pH and temperature, so it can be used at different pH levels and temperatures without affecting its function. 2-Chloro-4-nitrophenyl b-D-maltoheptaoside can be detected by titration calorimetry due to its high molecular weight. The molecule's affinity for carbohydrates also allows it to be used in optimization studies of carbohydrate molecules.</p>

<p>4-Chloro-3-indoxyl-1,3-diacetate is a fluorogenic or chromogenic substrate used in food testing. It is also an enzyme substrate that can be used to detect the presence of bioluminescence. 4-Chloro-3-indoxyl-1,3-diacetate has been shown to have high purity and high quality for use in diagnostic kits. 4-Chloro-3-indoxyl-1,3-diacetate is a ligand that is conjugated with other molecules to form complexes for use in fluorescence and chemiluminescence experiments.</p>

<p>6-Chloro-3-indoxyl choline phosphate is a chromogenic substrate for phosphatidylcholine-specific phospholipase C (PC-PLC) for the detection of mibrobial metabolites. The use of this specific substrate permits monitoring the activity of PC-PLC. This an important enzyme for the regulation of steady-state levels of phosphatidylcholine (PC) and diacylglycerol (DAG). Abnormally high levels of PC-PLC activity in various tissues have been linked to many disease progressions.</p>

<p>Chromogenic substrate for beta-galactofuranosidase</p>

<p>Phosphatase substrate</p>

<p>A substrate for alkaline phosphatase yielding a blue insoluble product upon cleavage. Colour can be further enhanced when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. The micronized structure enhances solubility.</p>

<p>5-Bromo-6-chloro-3-indolyl sulfate potassium salt is a fluorogenic substrate that can be used for food testing. It is a conjugate of 5-bromo-6-chloroindolylsulfate and potassium, which is dissolved in buffer solution. This product can also be used as a chromogenic substrate in environmental testing, an enzyme substrate in diagnostics, and a ligand for bioluminescence. It has high purity and quality, and can be used for chemiluminescence or bioluminescence. The CAS number for this product is 6581-24-4.</p>

<p>5-Bromo-3-indoxyl phosphate, disodium salt is an enzyme substrate that is used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate and food testing. It is also used as a ligand for diagnostic purposes. 5-Bromo-3-indoxyl phosphate, disodium salt has CAS number 16036-59-2 and it is soluble in water. This product has high quality and purity with a conjugate of 5% phenol red. 5-Bromo-3-indoxyl phosphate, disodium salt reacts with the enzyme horseradish peroxidase to produce a red color. This product can be used to detect hydrogen peroxide in the presence of an oxidizing agent such as hydrogen peroxide or potassium permanganate. The reaction produces a blue color when the solution is viewed in daylight or under ultraviolet light.</p>