Chromogenic Substrates

<p>4-Nitrophenyl (N-acetyl-a-neuraminosyl)-(2,3)-O-b-D-galactopyranosyl-(1,3)-2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of enzyme activity. It reacts with a specific enzyme to produce light. This product has been shown to be useful for detecting β -galactosidase, β -glucuronidase, α -mannosidase, and lactose permeases. The product is also used in the diagnosis of urinary tract infections and other diseases. This product is not intended for use in humans or animals.</p>

<p>4-Nitrophenyl 2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that contains a reactive group of 2,3,4-tri-O-levulinoyl. This compound can be used to measure the activity of enzymes such as beta-glucosidase and alpha-galactosidase. 4NP2OMP is an excellent ligand for metal ions and has been shown to bind to copper in the presence of ATP. 4NP2OMP can also be used as a diagnostic agent for measuring the activity of bacterial beta-glucosidase and alpha galactosidase.</p>

<p>Butyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring long chain leaving groups.</p>

<p>2-Chloro-4-nitrophenyl b-D-cellobioside (CNPG) is a chromogenic enzyme substrate that is commonly used to detect and measure the activity of β-glucosidases. CNPG is cleaved by the enzyme into the chromogenic product, which can be detected spectrophotometrically. β-glucosidases are important enzymes in the breakdown of cellulose, and CNPG is a widely used substrate for the detection and quantification of these enzymes in various research studies.</p>

<p>4-Nitrophenyl 4,6-O-isopropylidene-a-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of beta-galactosidase. This product is high purity, high quality and has a CAS number of 29781-31-5. It is an enzyme substrate with chromogenic and diagnostic properties that are used for conjugation to other molecules, as well as for culture media, chemiluminescence and food testing. 4NP4IPG is also used in bioluminescence assays to detect the presence of beta-galactosidase.</p>

<p>4-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-2-deoxy-b-D-glucopyranoside is a versatile chromogenic pNP enzyme substrate designed to offer superior results for detecting enzyme activity in research and diagnostic applications. This substrate enables faster, more accurate, and reliable detection of hydrolase enzymes, making it ideal for various research and testing needs, such as studying enzyme kinetics, screening inhibitors, and profiling enzyme specificity.</p>

<p>4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a synthetic compound with the ability to inhibit the enzyme glutamicum. It is a radionuclide that can be used as a focusing agent for gamma cameras and has been shown to be effective against corynebacterium glutamicum and other organisms. 4NP2AG was synthesized by reacting nitrophenol with acetamide in the presence of an acid catalyst and hydrogenating the product. The synthesis employs precession chemistry, which involves rotating a molecule around its axis at high speed to increase its concentration in one area. This treatment leads to a higher yield of desired product. 4NP2AG contains two acetamido groups on the glucopyranoside moiety, which are esterified with nitrophenol and acetic acid respectively. These moieties are</p>

<p>4-Nitrophenyl 6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside (4NBP) is a fluorogenic substrate for β-galactosidase. It has been used as an enzyme substrate in the detection of β-galactosidase activity. 4NBP is also used to detect the presence of galactose in culture media and as a chromogenic substrate in diagnostics.</p>

<p>2-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)]--2-deoxy--bDglucopyranoside is an acylated glucopyranoside that is used as a substrate for the enzyme β -galactosidase. When hydrolyzed by β -galactosidase, it yields a blue product that has been widely used in chemiluminescence and bioluminescence assays. It also can be used for staining and ligand binding studies.</p>

<p>Tetrabutylphosphonium p-toluenesulfonate is a fluorogenic substrate that is used in the diagnosis of cytochrome P450 enzymes. It is a ligand that binds to metal ions, such as copper and zinc. This compound is also used for the preparation of bioluminescent substrates for use in diagnostics and research.</p>

<p>4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.</p>

<p>2-Nitrophenyl laurate is a fatty acid that is not able to be degraded and has a high affinity for magnetic particles. It can be used in the immobilization of bacteria, such as Stenotrophomonas maltophilia, which are resistant to many antibiotics. 2-Nitrophenyl laurate also inhibits the enzyme lipolytic enzymes, which are responsible for the degradation of phospholipids in the cell membrane. This inhibition may lead to an increase in bacterial susceptibility to antibiotics. 2-Nitrophenyl laurate is a synthetic substrate that can be used to study the regulation of lipolytic enzymes by regulatory proteins.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic substrate designed for the detection and quantification of enzyme activity. This tetra-acetylated compound is specifically designed to be cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically, allowing for accurate and sensitive analysis of enzyme kinetics and inhibition studies.</p>

<p>Substrate for a-N-acetyl-galactosaminidase</p>

Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside is a versatile chromogenic substrate designed for the detection and quantification of various glycosidase enzymes. This substrate is widely used in enzyme assays, producing a yellow color upon cleavage by target enzymes. The 4-Nitrophenol released in this reaction can be easily monitored spectrophotometrically, allowing for rapid and accurate enzyme analysis. This high-quality substrate provides a convenient and reliable tool for researchers in biochemistry, enzymology, and related fields.</p>

<p>4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-benzylidene-b-D-glucopyranoside is a high purity, high quality 4nitrophenol derivative that is used as a food testing substrate. It is also used for the detection of fluorescence in environmental testing. This product can be conjugated with ligands for use in diagnostic assays.</p>

<p>4-Nitrophenyl 6-O-trityl-a-D-glucopyranoside is a high-quality trityl pNP substrate that performs excellently in various enzymatic assays. Containing a trityl-protected sugar moiety, this substrate is perfect for investigating alpha-D-glucopyranoside-specific enzymes and providing precise data for your experiments.</p>

<p>N-Acetyl-2-nitrophenyl 6-O-DMT-b-D-galactopyranoside is a fluorogenic substrate that is used in enzyme and chromogenic assays, including the determination of aminoglycoside activity. This product has been shown to be suitable for detection of bacterial contamination in food products and environmental samples. N-Acetyl-2-nitrophenyl 6-O-DMT-b-D-galactopyranoside reacts with aminoglycosides at the O4 position, forming a fluorescent product that can be measured using a fluorometer.</p>

<p>2-Methyl-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used in various applications. It is a ligand that binds to the enzyme beta-galactosidase and is used in diagnostics for detecting the presence of the enzyme. 2-Methyl-4-nitrophenyl b-D-galactopyranoside has been shown to be an appropriate substrate for chemiluminescence reactions, such as those used in diagnostic or research applications. The product also has been tested for potential toxicity and found not to be toxic at concentrations up to 100mg/mL. This product can be used in a variety of applications, including food testing, bacterial culture media, and environmental testing.</p>