Chromogenic Substrates

<p>S-(4-Nitrophenyl)mercapturic acid (S-NPMA) is the major metabolite of 4-nitrophenol, which is a precursor to dyes and pharmaceuticals. S-NPMA has been detected in urine samples from humans, rats, and mice. The main metabolic pathway for S-NPMA is an oxidative conversion by cytochrome P450 enzymes. S-NPMA has been found to be a useful biomarker for the presence of polycyclic aromatic hydrocarbons in wastewater and human urine. The levels of this compound have also been shown to increase with age in humans.</p>

<p>4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is an advanced chromogenic pNP enzyme substrate that delivers unparalleled performance in enzymatic assays. This high-quality substrate is specifically designed for studying glycosidases and other enzymes involved in carbohydrate metabolism. Our 4-Nitrophenyl 6-O-(a-D-glucopyranosyl)-a-D-glucopyranoside is synthesized with the highest quality materials to provide incredible accuracy and reliability in your research applications.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-(2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2,6,8,10,12-pentaoxoheptaacetic acid is a chemiluminescent substrate that is used for the detection of peroxidase and other enzymes. The reaction product is a yellow fluorescent product which can be measured using a fluorometer. It is sold as an aqueous solution at 1mg/mL in phosphate buffer at pH 7.4.</p>

<p>The 4-Nitrophenyl 2-acetamido-2-deoxy-4-O-[2-O-(a-L-fucopyranosyl)-b-D-galactopyranosyl]-b-D-glucopyranoside is a specialized pNP enzyme substrate designed to study fucosyl-galactose-dependent enzymes. This chromogenic substrate is valuable for researchers investigating glycosyltransferase activity, structure-function relationships of glycan-binding proteins, and regulation of cellular signaling pathways involving fucose modifications. Its high specificity and sensitivity ensure accurate measurements in diverse experimental setups, and its chromogenic detection system facilitates rapid and reliable analysis.</p>

<p>2,4-Dinitrophenyl b-D-cellobioside is a chemiluminescent substrate that is used to detect the activity of enzymes and other proteins. It can be conjugated with an antibody or other ligand for use in diagnostic purposes. 2,4-DNPBC is a pure compound with a high quality that does not contain any other impurities. Its CAS number is 157956-96-2.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(a-L-fucopyranosyl)-b-D-glucopyranoside is a fluorescent substrate for beta-galactosidase that is used in diagnostics and research. The product has been shown to be an excellent substrate for beta-galactosidase and beta-glucuronidase, as well as other glycosidases. It can be used to detect the presence of these enzymes in any sample containing glycosides. This product is also used in food testing, environmental testing, and bioluminescence experiments. 4NPAFG is available in high purity with a conjugate group at the C4 position.</p>

<p>b-Naphthyl-b-maltotrioside is a fluorogenic substrate that is used in enzymatic and chromogenic assays. It is a bifunctional substrate that reacts with enzymes to produce light. b-Naphthyl-b-maltotrioside can be used as a conjugate for immunoassays, such as ELISA. This substrate is also suitable for use in diagnostics, food testing, and environmental testing.</p>

<p>N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.</p>

<p>2-Chloro-4-nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-glucopyranoside is a chromogenic enzyme substrate used in the biochemical characterization of various enzymes. It produces a yellow color when cleaved by enzymes and is commonly used in assays to study enzyme kinetics and activity. It is commonly used is used to study the biochemical and physiological effects of glycosylation and acts as a substrate for glycosylation-dependent enzymes involved in carbohydrate metabolism. Glycosidases hydrolyse 2-Chloro-4-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranoside, releasing 2-chloro-4-nitrophenyl moiety, and the substrate can then be further metabolized by other enzymes. It is also used in the investigate the effects and activity on glycoproteins and glycolipids.</p>

<p>4-Nitrophenyl 2-O-(2,3,4-tri-O-acetyl-a-L-fucopyranosyl)-6-O-benzoyl-3,4-O-isopropylidene a D galactopyranoside is an analog of galactose that binds to the DNA and regulates gene expression. This compound has been shown to be able to regulate gene expression levels in cells. 4NP2B6OB was used as a probe for microarray analysis of gene expression profiles from environmental samples. It was found that 4NP2B6OB could be used as a probe for the identification of genes in high density arrays.</p>

<p>This product is a fluorogenic substrate that reacts with the enzyme beta-galactosidase to produce a fluorescent product. It can be used in food testing, environmental testing, and diagnostics. The CAS number for this product is 609-33-2. This product is available in high purity and high quality.</p>

<p>2-Chloro-4-nitrophenyl b-D-galactopyranoside is a chromogenic enzyme substrate used to study the activity of beta-lactoside. It produces a yellow color when cleaved by the enzyme, making it a useful tool in enzyme assays and screening experiments.</p>

2-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-galactopyranoside is a fluorescent substrate for β-galactosidase. It is used in food testing, environmental testing and diagnostics. This substrate is also used to detect the presence of β-galactosidase activity in cells and tissues. 2NPTABG provides a fluorescence signal that can be detected with an ultraviolet light or a luminometer. The luminescence intensity decreases when it reacts with β-galactosidase enzyme. This product has a CAS number of 3053-17-6 and is available at high purity levels.

<p>BCIP is a dye that can be used as a substrate in a fluorometric assay to detect the presence of effector proteins. It is also used in analytical chemistry to detect phosphate, and as a reagent in diagnostic tests for mucin gene expression. BCIP has been shown to have transcriptional regulatory effects on genes encoding for proteins involved in antibody production. BCIP has been shown to act as an effector protein, which influences the amount of mRNA produced by the gene encoding for tissue culture cells. BCIP can also be used as a model system to study the regulation of transcription in vitro.<br>A hybridization technique using BCIP with DNA-dependent RNA polymerase II has been demonstrated and applied to analyze the mechanism of transcriptional control at the level of individual genes. The use of this technique allows for analysis at a single gene level and may allow for better understanding of transcriptional regulation.</p>

<p>Octyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring hydrophobic leaving groups.</p>

<p>Substrate for alkaline phosphatase</p>

4-Nitrophenyl 2-acetamido-3-O-acetyl-4,6-O-benzylidene-2-deoxy-aDglucopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity. It is a water soluble substrate with an extinction coefficient of approximately 10,000 M−1 cm−1 at 420 nm. 4NPAABGDG is a chromogenic substrate that can be used to detect β-galactosidase activity. It has an extinction coefficient of approximately 1,700 M−1 cm−1 at 420 nm. The CAS number for 4NPAABGDG is 23262–56–8 and it is sold by Sigma Aldrich under catalog number L4959.

<p>N-Acetyl-L-glutamine p-nitroanilide is a fluorogenic substrate that can be used in the diagnosis of bacterial infections. It is a ligand that can bind to enzymes involved in the production of bioluminescence, such as luciferase, and can be used for the detection of bacteria. It is also used for food testing, environmental testing, and diagnostics. N-Acetyl-L-glutamine p-nitroanilide has high purity and high quality, which makes it suitable for conjugation with other molecules.</p>

<p>4-Nitrophenyl 4,6-benzylidene-3-O-(2,3,4,6-tetra-O-[4-methoxybenzyl]-a-D-glucopyranosyl)-a-D-glucopyranoside is a chemiluminescent substrate used for the detection of various enzymes. This substrate is commonly used in enzyme assays to measure the activity of β-galactosidase, α-amylase, and β-lactamase. The product can be used in diagnostic applications such as food testing and bacterial identification.</p>

6-Chloro-3-indolyl phosphate disodium salt is a substrate for visualising alkaline phosphatase activity. It produces a red colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.