5-Carboxytetramethylrhodamine succinimidyl ester

CAS: 150810-68-7

Ref. 3D-FC45651

Product Information

• Name: 5-Carboxytetramethylrhodamine succinimidyl ester
• Synonyms: 5-TAMRA SE
• Brand: Biosynth

Description

5-Carboxytetramethylrhodamine succinimidyl ester is a widely employed fluorescent dye (orange/red) that is suitable for different uses. In PCR applications, it has been used as both a reporter at the 5’ or as a quencher at the 3’ end. TAMRA-labelled oligonucleotides have been used to study interactions with DNA or DNA/protein interactions. It is an excellent fluorescent acceptor for FRET applications with a suitable dye pair. It is used as a protein/antibody labelling agent for structural studies, immunochemistry and flow cytometry. Bioconjugation is generally performed through coupling of the carboxylic acid to a primary amine at the N-terminus of a protein, on lysine side chains or amino-modified oligonucleotide. Carboxylic acids that are activated with succinimidyl esters react with primary amines.

Chemical properties

• Molecular weight: 527.52 g/mol
• Formula: C₂₉H₂₅N₃O₇
• Purity: Min. 95 Area-%
• Color/Form: Red Powder