Imidazole

CAS: 288-32-4

Ref. 3D-J-200340

Product Information

• Name: Imidazole
• Synonyms: Glyoxaline 1,3-Diaza-2,4-cyclopentadiene
• Brand: Biosynth
• Description: Imidazole is a highly polar aromatic compound with two annular nitrogen atoms. Imidazole is widely used in the affinity purification of proteins with polyhistidine tags (His-Tag). In the protein purification process, imidazole is used for the elution of His-Tag-fused recombinant proteins from a metal-immobilised resins. In the elution buffers, imidazole is typically used in the 50 – 500 mM concentration range (Bornhorst and Falke, 2000).

Chemical properties

• Molecular weight: 68.08 g/mol
• Formula: C₃H₄N₂