CAS 2348-71-2

[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]

Description:

[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], commonly known as MTT, is a yellow, water-soluble compound widely used in cell viability assays. It is a tetrazolium salt that, upon reduction by viable cells, forms a purple formazan product, allowing for quantification of cell metabolic activity. MTT is characterized by its ability to penetrate living cells and its non-toxic nature, making it suitable for in vitro studies. The compound is sensitive to light and should be stored in a cool, dark place to maintain stability. MTT is often utilized in various biological and pharmaceutical research applications, including cytotoxicity assays, proliferation studies, and drug screening. Its reduction to formazan is typically measured spectrophotometrically, providing a quantitative assessment of cell viability. The compound's structure includes a thiazole ring and diphenyl groups, contributing to its unique properties and reactivity. Overall, MTT is an essential tool in biochemistry and cell biology for evaluating cell health and metabolic function.

Formula: C₁₈H₁₆N₅S·Br

InChI: InChI=1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key: InChIKey=AZKSAVLVSZKNRD-UHFFFAOYSA-M

SMILES: CC=1N=C(N2[N+](=NC(=N2)C3=CC=CC=C3)C4=CC=CC=C4)SC1C.[Br-]

Synonyms:

• 2,5-Diphenyl-3-(4,5-dimethylthiazol-2-yl)-2H-tetrazolium bromide
• 2,5-Diphenyl-3-(4,5-dimethylthiazol-2-yl)tetrazolium bromide
• 2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazolium bromide
• 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide
• 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (1:1)
• 3,[4,5-Dimethylthiazol-2-YL]-2,5-DIPHENYLTETRAZOLIUM BROMIDE
• 3-(4,5-Dimethyl-2-THIAZOL-2-YL)-2,5-DIPHENYL-2H-TETRAZOLIUM BROMIDE
• 3-(4,5-Dimethyl-2-THIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE
• 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
• 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide
• 3-(4,5-Dimethylithiazolyl-2)-2,5-Diphenyl Monotetrazolium Bromide
• 3-(4,5-Dimethylthiazol-2-YL)-2,5-DIPHYL-2 H-TETRAZOLIUM BROMIDE
• 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-2H-Tetrazoliumbromide
• 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide
• 3-(4,5-Dimethylthiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
• 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide
• 3-(4,5-Dimethylthiazolyl-2)-2,5-DIPHENYL TETRAZOLIUM BROMIDE
• 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2,3-dihydro-1H-tetrazol-1-ium bromide
• 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide
• 4,5-Dimethythiazoyl-Zyl2,5-Diphenylterrajolium Bromiole
• MMT Tetrazolium
• MTT tetrazolium
• Methylthiazoletetrazolium
• Mtt (1.11714)
• NSC 60102
• Thiazolyl Blue
• Thiazolyl Blue Monotetrazolium
• Thiazolyl Blue tetrazolium bromide
• Thiazolyl blue (Sigma)

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide

CAS: 2348-71-2, 298-93-1

Formula: C₁₈H₁₆BrN₅S

Purity: >98.0%(T)

Color and Shape: Light yellow to Brown powder to crystal

Molecular weight: 414.33

Ref: 3B-D0801

MTT

CAS: 2348-71-2

Used in the MTT assay to asses the viability of cells. The yellow MTT is reduced by the living cells to a water-insoluble purple formazan. In order to make a quantitative measurement the formazan is often dissolved by addition of a solubilization solution to yield a colored solution that can be measured in a spectrophotometer. The rate of color formation reflects the dynamics and intensity of the microbial metabolism.

Formula: C₁₈H₁₇BrN₅S

Purity: Min. 95%

Molecular weight: 415.3 g/mol

Ref: 3D-CAA34871