CAS 2440-62-2

N-Succinyl-L-phenylalanine p-nitroanilide

Description:

N-Succinyl-L-phenylalanine p-nitroanilide is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of proteolytic enzymes such as trypsin. This compound features a succinyl group attached to the amino acid L-phenylalanine, which is further linked to a p-nitroanilide moiety. The presence of the p-nitroanilide group allows for colorimetric detection upon cleavage by enzymes, making it valuable in laboratory settings for enzyme activity studies. The compound is typically characterized by its solubility in organic solvents and its stability under standard laboratory conditions. Its molecular structure contributes to its specificity and reactivity, enabling it to serve as a reliable indicator in various biochemical applications. Safety precautions should be observed when handling this compound, as it may pose health risks if ingested or inhaled. Overall, N-Succinyl-L-phenylalanine p-nitroanilide is an important tool in enzymology and related fields.

Formula: C₁₉H₁₉N₃O₆

InChI: InChI=1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1

InChI key: InChIKey=KNBLWBFJHZHYFG-INIZCTEOSA-N

SMILES: [C@H](CC1=CC=CC=C1)(C(NC2=CC=C(N(=O)=O)C=C2)=O)NC(CCC(O)=O)=O

Synonyms:

• 4-({1-[(4-Nitrophenyl)Amino]-1-Oxo-3-Phenylpropan-2-Yl}Amino)-4-Oxobutanoic Acid
• 4-[[(1S)-2-[(4-Nitrophenyl)amino]-2-oxo-1-(phenylmethyl)ethyl]amino]-4-oxobutanoic acid
• Butanoic acid, 4-[[(1S)-2-[(4-nitrophenyl)amino]-2-oxo-1-(phenylmethyl)ethyl]amino]-4-oxo-
• Butanoic acid, 4-[[2-[(4-nitrophenyl)amino]-2-oxo-1-(phenylmethyl)ethyl]amino]-4-oxo-, (S)-
• N-Succinyl-<span class="text-smallcaps">L</span>-phenylalanine p-nitroanilide
• N-Succinyl-L-phenylalanine p-nitroanilide
• Nα-(3-Carboxypropionyl)-<span class="text-smallcaps">L</span>-phenylalanine-p-nitroanilide
• Succinamic acid, N-[α-[(p-nitrophenyl)carbamoyl]phenethyl]-, <span class="text-smallcaps">L</span>-
• Succinamic acid, N-[α-[(p-nitrophenyl)carbamoyl]phenethyl]-, L-

Suc-Phe-pNA

CAS: 2440-62-2

Substrate for chymotrypsin, also used for the identification of this enzyme by enzymoblotting.

Formula: C₁₉H₁₉N₃O₆

Purity: > 99%

Color and Shape: Light Yellow Powder

Molecular weight: 385.38

Ref: 01-4000089

4-((1-Benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl)amino)-4-oxobutanoic Acid

CAS: 2440-62-2

Formula: C₁₉H₁₉N₃O₆

Purity: 98%

Molecular weight: 385.3707

Ref: IN-DA00C1EU

4-({1-benzyl-2-[(4-nitrophenyl)amino]-2-oxoethyl}amino)-4-oxobutanoic acid

CAS: 2440-62-2

Formula: C₁₉H₁₉N₃O₆

Purity: 98% (TLC)

Molecular weight: 385.376

Ref: 10-F374651

Suc-Phe-pNA

CAS: 2440-62-2

Suc-Phe-pNA is a synthetic substrate that has been shown to be a proteolytic enzyme. It is used for the study of proteolytic enzymes and in pharmaceutical research because it can inhibit the activity of pancreatic enzymes such as trypsin or chymotrypsin. Suc-Phe-pNA has also been shown to have antiviral properties, which may be due to its ability to inhibit viral proteases. Suc-Phe-pNA is an inhibitor of serine protease, which is an enzyme that catalyzes reactions involving peptide bonds. The pH optimum for Suc-Phe-pNA is 8 and it has been shown to have a low inhibitory effect on trypsin activity at all pH levels.

Formula: C₁₉H₁₉N₃O₆

Purity: Min. 95%

Molecular weight: 385.37 g/mol

Ref: 3D-FS110741