CAS 35013-72-0

Biotin N-hydroxysuccinimide ester

Description:

Biotin N-hydroxysuccinimide ester, with the CAS number 35013-72-0, is a bioconjugation reagent commonly used in biochemical applications, particularly in the labeling of proteins and other biomolecules. This compound features a biotin moiety, which is a vitamin that plays a crucial role in cellular metabolism and is known for its strong affinity for avidin and streptavidin, making it invaluable in various assays and purification techniques. The N-hydroxysuccinimide (NHS) component enhances the reactivity of the ester, allowing it to form stable amide bonds with primary amines present in proteins or peptides. This characteristic is particularly useful for creating biotinylated proteins that can be easily isolated or detected. Biotin N-hydroxysuccinimide ester is typically soluble in organic solvents and exhibits stability under appropriate storage conditions. Its applications extend to areas such as immunology, molecular biology, and drug delivery systems, where precise labeling and tracking of biomolecules are essential for experimental success.

Formula: C₁₄H₁₉N₃O₅S

InChI: InChI=1/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI key: InChIKey=YMXHPSHLTSZXKH-RVBZMBCESA-N

SMILES: C(CCCC(ON1C(=O)CCC1=O)=O)[C@H]2[C@@]3([C@@](NC(=O)N3)(CS2)[H])[H]

Synonyms:

• (+)-Biotinyl-N-hydroxysuccinimide
• (N-Hydroxysuccinimidyl)biotin
• 1-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}oxy)pyrrolidine-2,5-dione
• 1-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]oxy}pyrrolidine-2,5-dione
• 1H-Thieno[3,4-d]imidazole, 2,5-pyrrolidinedione deriv.
• 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (3aS,4S,6aR)-
• 2,5-Pyrrolidinedione, 1-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]oxy]-, [3aS-(3aα,4β,6aα)]-
• 2,5-Pyrrolidinedione, 1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-
• <span class="text-smallcaps">D</span>-Biotin N-hydroxysuccinimide ester
• Biotin N-hydroxysuccinimide
• Biotin N-hydroxysuccinimidyl ester
• Biotin NHS ester
• Biotin succinimidyl ester
• Biotin-Nhs
• Biotin-OSu
• EZ-Link NHS-Biotin
• Iotin-Nhs
• N-Hydroxysuccinimidobiotin
• N-Succinimidyl <span class="text-smallcaps">D</span>-biotinate
• NHS Biotin
• NSC 345668
• Biotin N-hydroxysuccinimide ester
• D-BIOTIN, SE
• (+)-BIOTINYL-N-SUCCINIMIDYL ESTER
• BIOTIN (LONG ARM) NHS
• NHS-D-BIOTIN
• D-BIOTINYL-OSU
• BIOTIN SE
• BNHS
• D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
• D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
• 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER
• (+)-BIOTIN N-SUCCINIMIDYL ESTER
• BIOTINYL-N-HYDROXY-SUCCINIMIDE

N-Succinimidyl D-Biotinate

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purity: >97.0%(HPLC)(N)

Color and Shape: White to Almost white powder to crystal

Molecular weight: 341.38

Ref: 3B-S0491

(+)-Biotin N-succinimidyl ester, 98%

CAS: 35013-72-0

In coupling reactions to amino acids, peptides and proteins through primary amines

Formula: C₁₄H₁₉N₃O₅S

Purity: 98%

Color and Shape: Powder or crystals or crystalline powder, White to cream

Molecular weight: 341.38

Ref: 02-044771

D-Biotinyl-OSu

CAS: 35013-72-0

The activated biotin derivative Biotin-OSu is used for labeling the amino groups of amino acids, peptides, and proteins. When conducting the biotinylation at pH 6.5, N-terminal amino groups can be selectively tagged in the presence of lysine.

Formula: C₁₄H₁₉N₃O₅S

Purity: ≥ 97%

Color and Shape: White Powder

Molecular weight: 341.39

Ref: 01-4014225

2,5-Pyrrolidinedione,1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purity: 98%

Color and Shape: Solid

Molecular weight: 341.3828

Ref: IN-DA00I6XN

Biotin NHS

CAS: 35013-72-0

Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.

Formula: C₁₄H₁₉N₃O₅S

Purity: 98.27% - 98.63%

Color and Shape: Powder

Molecular weight: 341.38

Ref: TM-T14579

Biotin NHS

CAS: 35013-72-0

Biotin NHS

Purity: ≥98%

Molecular weight: 341.38g/mol

Ref: 54-BUP15883

(+)-Biotin N-hydroxysuccinimide ester

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Molecular weight: 341.40

Ref: 7W-GV8230

N-Hydroxysuccinimido biotin

CAS: 35013-72-0

N-Hydroxysuccinimido biotin

Purity: 98%

Color and Shape: White To Off White Powder

Molecular weight: 341.38g/mol

Ref: 54-BIB101

Biotin NHS

Controlled Product

CAS: 35013-72-0

Stability Moisture Sensitive
Applications Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.
References Ngo, T.T., et al.: Applied Biochem. Biotech, 7, 443 (1982), Wojchowski, D.M., et al.: J. Immunol. Methods, 90, 173 (1986);

Formula: C₁₄H₁₉N₃O₅S

Color and Shape: Neat

Molecular weight: 341.38

Ref: TR-S689500

Biotin succinimidyl ester

CAS: 35013-72-0

Biotinylation reagent for labeling nucleic acids or proteins.

Formula: C₁₄H₁₉N₃O₅S

Purity: Min. 98 Area-%

Color and Shape: White Off-White Powder

Molecular weight: 341.38 g/mol

Ref: 3D-FB10303

Biotinyl-OSu

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purity: 95%

Color and Shape: Solid, Off-white powder

Molecular weight: 341.38

Ref: 10-F330592

NHS-Biotin

CAS: 35013-72-0

NHS-Biotin is a biotin analog that can be used as an affinity label for the purification of specific proteins. NHS-Biotin is synthesized by reacting monoclonal antibody with lysine residues and a hydroxyl group, which form a covalent bond known as an amide linkage. The NHS-Biotin can then be covalently attached to the protein of interest. This attachment allows the protein to be purified by passing it over a column of immobilized streptavidin. NHS-Biotin has been shown to bind to actin filaments in humans and uptake peptide hormones in the bloodstream. It was also found that human serum contains chemical reactions with NHS-Biotin, forming a disulfide bond between two cysteine residues on the protein.

Formula: C₂₈H₃₉F₃N₆O₉

Purity: Min. 95%

Molecular weight: 660.64 g/mol

Ref: 3D-DPG-5707

Biotin-OSu

CAS: 35013-72-0

Biotin-OSu is a biotin derivative that is used as a fluorescence probe for the detection of DNA binding activity. It has been shown to bind to DNA with high affinity in an experimental model. Biotin-OSu binds to the major groove of DNA and can be used to detect dna binding activities, such as protein-DNA interactions, by forming a ternary complex with the target protein and a fluorophore. This compound has also been shown to react with mouse monoclonal antibodies and human serum proteins, which are transfer reactions. Biotin-OSu has also been used as a coupling agent in peptide hormones and streptavidin polymerase chain reactions (PCR). The presence of disulfide bonds in this compound give it stability but limits its solubility.

Formula: C₁₄H₁₉N₃O₅S

Purity: Min. 95%

Molecular weight: 341.39 g/mol

Ref: 3D-BOS-6003-PI