CAS 4091-99-0: 2′,7′-Dichlorodihydrofluorescein diacetate

Description: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent, cell-permeable compound widely used as a probe for detecting reactive oxygen species (ROS) in biological systems. Upon entering cells, DCFH-DA is hydrolyzed by intracellular esterases to form dichlorodihydrofluorescein (DCFH), which can then be oxidized by ROS to produce a highly fluorescent compound, dichlorofluorescein (DCF). This transformation allows for the quantification of oxidative stress within cells. DCFH-DA is characterized by its stability, ease of use, and ability to provide real-time measurements of ROS levels. It is typically utilized in various applications, including cell culture studies, flow cytometry, and fluorescence microscopy. The compound is soluble in organic solvents and has a relatively low toxicity profile, making it suitable for in vitro experiments. However, care must be taken to avoid light exposure during experiments, as fluorescence can be affected by photobleaching. Overall, DCFH-DA is a valuable tool in the field of cellular biology and oxidative stress research.

Formula: C₂₄H₁₆Cl₂O₇

InChI: InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key: InChIKey=PXEZTIWVRVSYOK-UHFFFAOYSA-N

SMILES: O=C(O)C=1C=CC=CC1C2C3=CC(Cl)=C(OC(=O)C)C=C3OC4=CC(OC(=O)C)=C(Cl)C=C42

Synonyms:

• 2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate
• 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
• 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
• 2′,7′-Dichlorodihydrofluorescein diacetate
• Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
• Benzoic acid, o-(2,7-dichloro-3,6-dihydroxyxanthen-9-yl)-, diacetate
• Dcfh-Da
• H2Dcfda
• H₂DCFDA
• DCFH

2',7'-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: >97.0%(T)(HPLC)
• Color and Shape: White to Light yellow powder to crystal
• Molecular weight: 487.29

H2DCFDA

CAS: 4091-99-0

H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for …

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: 98.06% - 99.47%
• Color and Shape: Solid
• Molecular weight: 487.29

Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-

CAS: 4091-99-0

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: 97%
• Color and Shape: Solid
• Molecular weight: 487.2856

2?,7?-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

• Purity: 97%
• Molecular weight: 487.29g/mol

H2DCFDA

CAS: 4091-99-0

• Purity: ≥98%
• Molecular weight: 487.29g/mol

2'',7''-Dichlorofluorescin diacetate

CAS: 4091-99-0

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: ≥ 95.0%
• Color and Shape: White to off-white powder
• Molecular weight: 487.29

2,7-Dichlorodihydrofluorescein diacetate

CAS: 4091-99-0

• Purity: 97.0%
• Color and Shape: Liquid, No data available.
• Molecular weight: 487.2900085449219

2',7'-Dichlorodihydrofluorescein diacetate

CAS: 4091-99-0

Cell permeable reagent for use in detection of peroxidase activity

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: Min. 97 Area-%
• Color and Shape: Powder
• Molecular weight: 487.29 g/mol

2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97%

CAS: 4091-99-0

• Formula: C₂₄H₁₆Cl₂O₇
• Purity: min. 97%
• Color and Shape: White to off - white to pale yellow, Crystalline powder
• Molecular weight: 487.29

2',7'-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

Stability Light Sensitive Applications 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and …

• Formula: C₂₄H₁₆Cl₂O₇
• Color and Shape: Colourless
• Molecular weight: 487.29