CAS 4091-99-0

2′,7′-Dichlorodihydrofluorescein diacetate

Description:

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent, cell-permeable compound widely used as a probe for detecting reactive oxygen species (ROS) in biological systems. Upon entering cells, DCFH-DA is hydrolyzed by intracellular esterases to form dichlorodihydrofluorescein (DCFH), which can then be oxidized by ROS to produce a highly fluorescent compound, dichlorofluorescein (DCF). This transformation allows for the quantification of oxidative stress within cells. DCFH-DA is characterized by its stability, ease of use, and ability to provide real-time measurements of ROS levels. It is typically utilized in various applications, including cell culture studies, flow cytometry, and fluorescence microscopy. The compound is soluble in organic solvents and has a relatively low toxicity profile, making it suitable for in vitro experiments. However, care must be taken to avoid light exposure during experiments, as fluorescence can be affected by photobleaching. Overall, DCFH-DA is a valuable tool in the field of cellular biology and oxidative stress research.

Formula: C₂₄H₁₆Cl₂O₇

InChI: InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key: InChIKey=PXEZTIWVRVSYOK-UHFFFAOYSA-N

SMILES: C(O)(=O)C1=C(C2C=3C(OC=4C2=CC(Cl)=C(OC(C)=O)C4)=CC(OC(C)=O)=C(Cl)C3)C=CC=C1

Synonyms:

• 2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate
• 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
• 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid
• 2′,7′-Dichlorodihydrofluorescein diacetate
• Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
• Benzoic acid, o-(2,7-dichloro-3,6-dihydroxyxanthen-9-yl)-, diacetate
• Dcfh-Da
• H2Dcfda
• H₂DCFDA
• DCFH

2',7'-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

Formula: C₂₄H₁₆Cl₂O₇

Purity: >97.0%(T)(HPLC)

Color and Shape: White to Light yellow powder to crystal

Molecular weight: 487.29

Ref: 3B-D4409

Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-

CAS: 4091-99-0

Formula: C₂₄H₁₆Cl₂O₇

Purity: 97%

Color and Shape: Solid

Molecular weight: 487.2856

Ref: IN-DA00I80J

H2DCFDA

CAS: 4091-99-0

H2DCFDA (DCFH-DA) belongs to the class of green fluorescent dyes and is a probe for the detection of intracellular reactive oxygen species (ROS) (Ex/Em=488/525

Formula: C₂₄H₁₆Cl₂O₇

Purity: 97.04% - 99.47%

Color and Shape: White Solid

Molecular weight: 487.29

Ref: TM-T15458

2',7'-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

2',7'-Dichlorodihydrofluorescein Diacetate

Formula: C₂₄H₁₆Cl₂O₇

Purity: 97%

Color and Shape: Solid

Molecular weight: 487.29

Ref: 54-OR1010239

H2DCFDA

CAS: 4091-99-0

H2DCFDA

Formula: C₂₄H₁₆Cl₂O₇

Purity: ≥95%

Color and Shape: Solid

Molecular weight: 487.29

Ref: 54-BUP16178

2'',7''-Dichlorofluorescin diacetate

CAS: 4091-99-0

Formula: C₂₄H₁₆Cl₂O₇

Purity: ≥ 95.0%

Color and Shape: White to off-white powder

Molecular weight: 487.29

Ref: 7W-GW2593

H2DCFDA

CAS: 4091-99-0

2,7-Dichlorodihydrofluorescein diacetate

Formula: C₂₄H₁₆Cl₂O₇

Purity: 97%

Molecular weight: 487.29

Ref: IN-DG221268

2',7'-Dichlorodihydrofluorescein Diacetate

CAS: 4091-99-0

Stability Light Sensitive
Applications 2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
References Lebel, C., et al.: Chem. Res. Toxicol., 5, 227 (1992), Kjaer, M., et al.: Lipids, 43, 813 (2008), Baron, C., et al.: J. Agric. Food Chem., 57, 4185 (2009),

Formula: C₂₄H₁₆Cl₂O₇

Color and Shape: Neat

Molecular weight: 487.29

Ref: TR-D434215

2',7'-Dichlorodihydrofluorescein diacetate

CAS: 4091-99-0

Cell permeable reagent for use in detection of peroxidase activity

Formula: C₂₄H₁₆Cl₂O₇

Purity: Min. 97 Area-%

Color and Shape: Powder

Molecular weight: 487.29 g/mol

Ref: 3D-FD74827

2,7-Dichlorodihydrofluorescein diacetate

CAS: 4091-99-0

Formula: C₂₄H₁₆Cl₂O₇

Purity: 97%

Color and Shape: Liquid, No data available.

Molecular weight: 487.29

Ref: 10-F544672

2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97%

CAS: 4091-99-0

Formula: C₂₄H₁₆Cl₂O₇

Purity: min. 97%

Color and Shape: White to off - white to pale yellow, Crystalline powder

Molecular weight: 487.29

Ref: SR-85048