Enzyme

<p>selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity.  It has also been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.</p>

<p>Myokinase (Adenylate kinase, EC 2.7.4.3) catalyzes interconversion between ATP, ADP and AMP by catalyzing the following reaction:ATP + AMP ⇌ 2 ADPOne unit of Myokinase will convert 1.0 µmol ATP and 1.0 µmol AMP to 2.0 µmol ADP per min at 25°C and pH 7.5.</p>

<p>Carbonic anhydrase inhibitor 6 is an hCA inhibitor that inhibits hCA IX, hCA II, hCA XII, and hCA I. It is used in the study of lupus erythematosus.</p>

<p>Lyticase is a lysing enzyme that is designed to lyse cells in a biological sample. It contains an optimized wild-type guanine nucleotide-binding protein and has been shown to have high enzyme activities. Lyticase has also been shown to be active against opportunistic fungal strains, such as Candida glabrata, by disrupting their cell membranes. Lyticase is classified as a signal peptide with nuclear DNA, which allows it to be used in wastewater treatment applications. The enzyme can also be used for the analysis of the Toll-like receptor (TLR) response of microbes due to its electrochemical impedance spectroscopy properties.</p>

<p>CAXII-IN-1, antitumor, selectively inhibits CA XII with Ki of 3.8 nM for hCA XII and 56 nM for hCA IX.</p>

<p>LX-1031 is an effective inhibitor of tryptophan 5-hydroxylase. LX-1031 decreases serotonin (5-HT) synthesis peripherally.</p>

<p>Diethyl-pythiDC is an collagen prolyl 4-hydroxylase inhibitor.</p>

<p>Compound 1e, also known as ALP/Carbonic anhydrase-IN-1, is a dual inhibitor targeting both carbonic anhydrase (CA) isozymes II, IX, and XII, as well as alkaline</p>

<p>CA Inhibitor 12 strongly blocks CA II (K_i 1.72 nM), also inhibits CA I (271 nM), shows anticancer effects.</p>

<p>hCAIX-IN-15 is a potent inhibitor of human carbonic anhydrase IX (hCA IX) with an inhibition constant (Ki) of 38.8 nM, exhibiting broad-spectrum anticancer</p>

<p>N-Desethyl Brinzolamide oxalate functions as a dual inhibitor targeting Carbonic anhydrase II and Carbonic anhydrase IV, exhibiting inhibitory concentrations (IC50) of 1.28 nM and 128 nM, respectively [1].</p>

<p>hCAIX/XII-IN-8 (compound 3g) is a potent inhibitor of the human carbonic anhydrases (CAs) IX and XII, with inhibition constants (K i) of 8.5 nM for CA IX and 6.</p>

<p>hCAIX-IN-16 (Compound 12d), an inhibitor of hCA IX, exhibits inhibition constants (K i) of 190.0 nM for hCA IX and 187.9 nM for hCA XII.</p>

<p>COX-2-IN-30, a benzenesulfonamide derivative, is an orally active, dual inhibitor of cyclooxygenase-2 (COX-2; IC50 = 49 nM) and cyclooxygenase-1 (COX-1; IC50 =</p>

<p>Oxalate Oxidase, freeze-dried, is an enzymatic preparation that serves as a catalyst in biochemical reactions. This enzyme is derived from wheat, a common plant source, ensuring a naturally occurring origin. Its primary mode of action is the oxidation of oxalate into carbon dioxide and hydrogen peroxide. This biochemical activity is significant in various scientific applications, specifically in the breakdown of oxalate, which plays a crucial role in metabolic and environmental processes.</p>

<p>Sulfatase from Helix pomatia is a highly potent enzyme that is capable of hydrolyzing sulfated compounds and sulfate esters. It has been widely used in various applications such as glucosinolate analysis, genistein extraction preparation, and regiospecificity studies. With a concentration of ≥10,000 units per gram of solid, this sulfatase offers exceptional enzymatic activity for sulfatase assays. It effectively catalyzes the hydrolysis of sulfated substrates, including p-nitrocatechol sulfate, naphthyl sulfate and phenyl sulfates.The enzyme can be incubated with the desired sample to facilitate the release of sulfate groups from sulfated compounds. Sulfatase from Helix pomatia is a valuable tool for researchers and scientists working in diverse fields requiring efficient and reliable enzymatic hydrolysis capabilities. Additionally the enzyme has been found to have industrial applications, such as in the bioconversion of industrial chemicals, where it can be used as a catalyst.</p>

<p>p-Ethynylphenylalanine (4-Ethynyl-L-phenylalanine), a tryptophan hydroxylase (TPH) inhibitor, is competitive, effective, selective, and reversible, with a Ki of</p>

<p>Phosphodiesterase II from bovine spleen is an enzyme derived from the spleen of cattle, which serves as a crucial biological catalyst for the hydrolysis of phosphodiester bonds in nucleotide sequences. This enzyme's mode of action involves cleaving the phosphodiester linkages within nucleic acids, facilitating the breakdown of these macromolecules into smaller nucleotide units.</p>

<p>Thioredoxin reductase (TR, TrxR) (EC 1.8.1.9) is an enzyme that reduce thioredoxin using NADPH as a co-factor, and also contains FAD. One unit of thioredoxin reductase will raise increase light absorbance by 1.0 per minute at 412nm in the presence of thioredoxin and Ellman's reagent at pH 7.0 and 25 °C.</p>

<p>Glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the following reaction:  glycerol-3-phosphate + O2 ⇌ dihydroxyacetone phosphate + H2O2  One unit of Glycerol-3-phosphate oxidase will generate 1.0 μmole H2O2 per min at 37°C, under the presence of O2 and the optimal pH. If required, you can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Glycerokinase (glycerol kinase, GP, ATP-glycerol 3-phosphotransferase; EC 2.7.1.30) is an enzyme that catalyzes the following reaction:  ATP + glycerol ⇌ ADP + glycerol 3-phosphate  One unit of Glycerokinase will convert 1.0 μmole of glycerol and ATP to glycerol 3-phosphate and ADP per min at pH 9.8 and 25 °C.</p>

<p>Triose-phosphate isomerase (TPI, TIM; EC 5.3.1.1) is an enzyme that catalyzes the reversible isomerisation of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate:  DHAP ⇌ GADP  The reaction mechanism involves the formation of an enediol intermediate. One unit of Triose-phosphate isomerase will convert 1.0 μmole glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 and 25 °C.</p>

<p>Cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the following reaction: cholesterol + O2 ⇌ cholest-4-en-3-one + H2O2One unit of cholesterol oxidase will convert 1.0 μmole of cholesterol into cholest-4-en-3-one per min at pH 7.5 and 25 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Poly(ethylene terephthalate) hydrolase is an enzyme involved in the breakdown of Polyethylene terephthalate which is present in many plastics Polyethylene terephthalate hydrolytic enzymes may be useful in biotechnology, for use in waste treatment, biocatalysis and biorecycling</p>

<p>Sarcosine oxidase (Monomeric sarcosine oxidase, MSOX, EC 1.5.3.1) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2 and formaldehyde in the following reaction:  CH3-NH2+-CH2-COO- + H2O + O2 → NH3+-CH2-COO- + H2O2 + CH2O or sarcosine + water + oxygen → glycine + hydrogen peroxyde + formaldehydeOne unit of Sarcosine oxidase will form 1.0 micromole of formaldehyde from sarcosine per minute at pH 8.3 and 37 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Carbonic anhydrase inhibitor 3 (compound 11g) is an inhibitor of carbonic anhydrase II that reduces the intraocular pressure in glaucomatous rabbits [1].</p>

<p>4-Acetylphenylboronic acid acts as an effective inhibitor targeting carbonic anhydrase II (CAII), displaying inhibitory concentrations (IC50) of 246 μM for bovine CAII (bCA II) and 281.40 μM for human CAII (hCA II).</p>

<p>Tyrosinase-IN-20 (compound 6a) acts as a potent Tyrosinase inhibitor, demonstrating an IC 50 value of 28.50 μM [1].</p>

<p>Sulclamide, a sulfamoylbenzoic acid derivative, exhibits diuretic activity and functions as an inhibitor of carbonic anhydrase [1].</p>

<p>hCAVII/IX-IN-1 (compound 4) functions as an inhibitor of hCAVII/IX, exhibiting Ki values of 56.5 nM and 38.2 nM, respectively. It is applicable in the field of cancer research.</p>

<p>CAII-IN-1 (3n) is a selective bovine CA-II inhibitor with 10.3 μM IC50, used in carbonic anhydrase disorder studies.</p>

<p>β-Glucuronidase/hCAII-IN-2 is a potent inhibitor of β-glucuronidase and hCA II, and their IC50 values were 670.7 μM and 21.77 μM, respectively.</p>

<p>Carbonic anhydrase inhibitor19 (compound 26a) targets glaucoma-associated isozymes hCA II and hCA XII, with inhibition constants (Kis) of 9.4 nM and 6.7 nM, respectively. This compound is effective in reducing intraocular pressure.</p>

<p>Compound 7c inhibits carbonic anhydrase II, lowering intraocular pressure in glaucomatous rabbits.</p>

<p>CAII-IN-3, a thiosemicarbazone, potently inhibits CA-II with an IC50 of 13.4 μM.</p>

<p>hCAII-IN-4 (Compound 12j) is a potent inhibitor of human carbonic anhydrase II (hCA II), exhibiting an inhibitory concentration (IC50) of 7.78 μM.</p>

<p>α-Glucosidase-IN-63 (Compound 4d) serves as an α-Glucosidase inhibitor with an IC 50 value of 0.44 μM. Additionally, it exhibits inhibitory activity against hCA II, demonstrating a K i of 7.0 nM. The compound is also effective when administered orally. [1]</p>

<p>hCAII-IN-3 inhibits key hCA isoforms with Ki: hCA I (403.8 nM), hCA II (5.1 nM), hCA IX (10.2 nM), hCA XII (5.2 nM); shows anticancer potential.</p>

<p>hCAIX/XII-IN-15 (Compound 17β) is an inhibitor of hCA IX and hCA XII, exhibiting Ki values of 0.42 and 4.37 μM, respectively. It demonstrates a pro-apoptotic effect in multiple myeloma cells.</p>

<p>Tyrosinase-IN-37 (Compound 3c) is a potent inhibitor of tyrosinase, with an IC50 value of 1.02 μM, which is 14 times more effective than kojic acid (IC50 of 14.74 μM). This compound effectively prevents the browning of Rosa roxburghii and can also inhibit browning not caused by tyrosinase.</p>

<p>Tyrosinase-IN-29 (compound 5c) is an effective inhibitor of abTYR tyrosinase, demonstrating an IC50 value of 6.11 μM. It is suitable for further research into the inhibition of excessive skin pigmentation.</p>

<p>O-Desmethyl Brinzolamide hydrochloride (compound 6a), a potent metabolite derived from Brinzolamide, serves as a carbonic anhydrase (CA) inhibitor. It exhibits a dissociation constant (Kd) of 0.136 nM for CA II and an inhibitory concentration (IC50) of 165 nM for CA IV [1].</p>

<p>Carbonic anhydrase inhibitor 16 (compound 1) is a CA I/CA II inhibitor with potential antiviral activity, used in virus infection studies.</p>

<p>Tyrosinase-IN-33 (compound 5), a pyridine-based compound, acts as an effective inhibitor of diphenolase activity in mushroom tyrosinase. It significantly reduces enzyme activity with an IC50 of 9.0 μM.</p>

<p>Tyrosinase-IN-35 (compound 6g), exhibiting a IC 50 value of 2.09 μM, serves as a more effective inhibitor of human tyrosinase compared to Kojic Acid (IC 50: 16.38 μM). At concentrations of 4 μM and 8 μM, this compound effectively lowers melanin levels in melanoma B16F10 cells in vitro.</p>

<p>GSK 366 is a potent kynurenine-3-monooxygenase (KMO) inhibitor (IC50s: 0.7 nM and 2.3 nM for P. fluorescens-KMO and human KMO).</p>

<p>Perfluorohexane sulfonamide (FHxSA) serves as an inhibitor of carbonic anhydrase (CA), effectively inhibiting bovine CA and human CAII with IC50 values of 0.122 and 1.38 μM, respectively. Additionally, it acts as a delayed-action insecticide for controlling red imported fire ants (Solenopsis invicta). Furthermore, Perfluorohexane sulfonamide is considered a potential environmental pollutant.</p>

<p>AChE/CA I-IN-1 (Compound 2g) acts as an inhibitor for both AChE and hCA I, with Ki values of 1.85 µM and 0.53 µM, respectively. It has shown potential applications in the research of Alzheimer's disease, glaucoma, and epilepsy.</p>

<p>Tyrosinase-IN-39 (compound 5r) is a competitive inhibitor of tyrosinase, with an IC50 of 6.4 μM, and is used in the study of skin diseases.</p>

<p>Isobutylamido thiazolyl resorcinol is a tyrosinase (Tyrosinase) inhibitor that prevents pigment deposition induced by ultraviolet radiation.</p>