Biochemicals and Reagents

<p>mTOR serine/threonine kinase inhibitor</p>

<p>H-VYLKTNVAL-OH is a custom peptide that can be synthesized rapidly by our peptide experts in under 2 weeks. H-VYLKTNVAL-OH is provided at greater that &gt;95% purity (HPLC) from Cymit Quimica for a variety of research applications. We also offer H-VYLKTNVAL-OH in bulk quantities in addition to our standard pack sizes.Please enquire for more information about H-VYLKTNVAL-OH at the technical inquiry form on this page</p>

<p>Amyloid β-peptide (Aβ) has been identified as the key subunit of the extracellular plaques found in the brains of patients with Alzheimer's disease (AD) and Down's syndrome (DS). Aβ has therefore been extensively studied as a potential target for treatment of AD.Aβ is formed from the cleavage of the large, transmembrane protein- APP (amyloid precursor protein). Cleavage of APP by β- and then γ-secretases results in the formation of Aβ. Aβ can aggregate to produce amyloid-β oligomers, which are thought to be highly neurotoxic. Over time Aβ can further aggregate to produce the characteristic senile plaques present in AD and DS.Aβ can be degraded by enzymes such as neprilysin, insulin degrading enzyme or endothelin converting enzyme. At physiological levels Aβ may be involved in controlling synaptic activity and neuronal survival.Aβ1-40 is a major C terminal variant of amyloid β constituting the most abundant AB peptide in the human brain.</p>

<p>TPX0131 is a recombinant human anti-IL8 antibody. TPX0131 has been shown to inhibit IL8 induced neutrophil chemotaxis and to block the IL8 receptor. TPX0131 also blocks the activation of NF-κB, a transcription factor involved in inflammation, and inhibits IL8 induced expression of adhesion molecules on endothelial cells. The affinity of TPX0131 for IL8 is low (Kd=1.5 nM) and it has high purity (&gt;98%).</p>

<p>Rabbit polyclonal CPT1B antibody</p>

<p>The heat shock protein 27 (HSP27) is a protein chaperone which ensure that damaged proteins are refolded into their correct conformation in the event of heat shock or other stress stimuli. In the event of oxidative stress HSP27 increases glutathione and decreases iron levels thus acting as an antioxidant. Further roles of HSP27 include, the inhibition of the apoptotic process through binding to the apoptotic proteins Bax, cytochrome C and DAXX and it also controls the actin cytoskeleton.HSP27 itself is regulated by the phosphorylation of its serine residues and this phosphorylation can alter the properties of HSP27. For example in its role in the actin cytoskeleton, when HSP27 is phosphorylated it stimulates actin filament polymerisation. Conversely when it is not phosphorylated HSP27 is an actin capping protein.HSP27 has the potential to be used as a biomarker in renal disease due to its levels being increased in renal fibrosis. It may also be used as a therapeutic target to treat cancers such as prostate carcinoma and breast cancer as it promotes tumour cell survival through inhibition of the apoptotic process. Moreover HSP27 may contribute to the treatment of neuro-degenerative diseases in that they can prevent the accumulation of misfolded proteins.</p>

<p>Lys-des-Arg(9) bradykinin is a metabolite of des-Arg(9)kinin and its production is increased upon inflammation. Lys-des-Arg(9) bradykinin acts on the bradykinin B(1) receptor as a potent and highly selective receptor agonist.The bradykinin receptor B1Rs is a heterotrimeric G-protein coupled receptor which regulates migration of microglia and T cells. The expression of the B(1) receptor is induced in various inflammatory conditions, and may play an important role in modulating cardiovascular function.The phenylalanine-residue at positions 6 of this peptide is isotopically labelled with carbon-13 (9) and nitrogen-15 (1), giving this peptide a mass increase of 10 compared to the unlabelled peptide.</p>

<p>DDOST (47-57) (heavy) is derived from dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST). Congenital disorders of glycosylation are associated with functionally inactive DDOST. The arginine residue at position 11 is isotopically labelled with carbon-13 (6) and nitrogen-15 (4).</p>

<p>[6-FAM]-Arg8 is an arginine rich cell penetrating peptide labelled with 6-carboxyfluorescein (6-FAM).</p>

<p>Ovalbumin (OVA) is the primary protein in egg-white, and is involved in initiating food allergies and asthma. It is a highly immunogenic protein and can be used for peptide conjugation in the development of antibodies.OVA (324-340) is a class I (Kb)-restricted peptide epitope of OVA. The ovalbumin fragment is presented by the class I MHC molecule, H-2Kb.</p>

<p>ACE I is a peptidyl-dipeptidase that has been well studied due to its crucial role in blood pressure regulation- ACE I converts angiotensin II to angiotensin I plus degradation of bradykinin as part of the circulating renin-angiotensin system (RAS). ACE I is involved in age-related neurodegeneration. Deregulation of dopamine is evident in Alzheimer's disease and Parkinson's disease with links to RAS. Acein has been shown to interact with ACE I with high affinity without effecting peptidase activity. Furthermore, acein was shown to stimulate dopamine release from rat brain tissue in vitro and in vivo. The mechanism of action has yet to be uncovered.</p>

<p>AF12198 is a selective receptor antagonist to the human cytokine, type 1 interleukin-1 (IL-1) and thus blocks IL-1β signalling. AF12198 can inhibit IL-1-induced IL-8 production by human dermal fibroblasts and IL-1-induced intercellular adhesion molecule-1 (ICAM-1) expression by endothelial cells. AF12198 also blocks IL-1 induction of IL-6 and down regulates IL-6 induction in monkeys. AF12198 does not bind the human type II IL-1 receptor, or the murine type I IL-1 receptor.IL-1 influences a wide range of immune and inflammatory responses. Sustained expression of even low levels of IL-1 can be harmful in chronic inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease.</p>

<p>Peptide H-I^LGGQE-OH is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>HIF-1 alpha (556-574) is derived from hypoxia-inducible factor 1-alpha (HIF-1 alpha), a subunit of the heterodimeric transcription factor HIF-1 and is responsible for maintaining oxygen homeostasis. HIF-1 alpha is expressed under hypoxic conditions and is oxygen sensitive. Hypoxia can occur in cancer, heart disease and pulmonary disorders.Structurally HIF-1 alpha contains a N-terminal transactivation domain (N-TAD) which stabilises HIF-1 alpha and a C-terminal transactivation domain (C-TAD) which under hypoxic conditions regulates the transcription of HIF-1 alpha. Moreover HIF-1 alpha features an oxygen dependent degradation domain containing two proline residues and a lysine532. The two proline residues are substrates of prolyl-4-hydroxylases (PHDs) which in the presence of sufficient oxygen, are hydroxylated. Similarly the lysine residue is acetylated by arrest-defective-1 and this is reduced in hypoxic conditions. These hydroxylated prolines and acetylated lysine target HIF-1 alpha for ubiquitination and degradation.</p>

<p>Galanin is a widely distributed neuropeptide in the central nervous system, peripheral regions and endocrine system. Galanin has a role in energy homeostasis- central injections of galanin to the amygdala led to food intake in rats. Galanin also acts in the CNS to inhibit neurotransmitter release, such as acetylcholine. Galanin has been implicated in numerous neurological conditions, including Alzheimer's disease, depression, and epilepsy.Galanin interacts with 3 receptor subtypes, GalR1-3 G protein-coupled receptors inserted into the plasma membrane. GalR1 is believed to activate a Gβγ pathway to regulate MAPK activation. GalR2 can also activate the MAPK pathway, but unlike GalR1, there is detectable inositol phosphate production. GalR3 is associated with the Galphai/o pathway. Activation of the receptor leads to a cellular influx of potassium ions.The galanin active fragment 1-16 has been identified as a highly potent agonist for the galanin receptors.  These have become a basis for galanin-based peptides, which are neuroactive. These are being investigated as a potential source for anticonvulsant neuropeptides as a therapeutic for conditions such as epilepsy. A library of galanin fragments has allowed screening of their properties to be assessed and used to generate chimeric peptides. Galanin fragments have different affinities for GalR receptors however, the N-terminal 1-16 have been shown to have a conserved affinity for the receptors. This galanin (2-13) peptide is provided in the acidic form. The amide form is also available in our catalogue.</p>

<p>Sheep polyclonal Zearalanone antibody</p>

<p>The ionic-complementary octapeptide, FEFEFKFK, can self-assemble into antiparallel β-sheet rich fibres and forms very stable hydrogels when at a concentration of more than 20 mg/mL (in water).These peptide hydrogels are naturally biocompatible and biodegradable and can be metabolised by the body. Such hydrogels have been shown to mimic the structure of the extracellular matrix, thus offering cells a niche to undertake their physiological functions. These properties mean that these hydrogels have the potential for use in medical applications.FEFEFKFK hydrogels are able to support the culture of cells such as mesenchymal stem cells in three dimensions with sustained cell viability. They can also support the differentiation into osteoblasts and promote mineralisation upon addition of osteogenic stimulation. They therefore have potential for use in the regeneration of hard tissues such as alveolar bone following injury or degeneration.</p>

<p>The acetylated N-terminal peptide of murine myelin basic protein (MBP) (1-9).</p>

<p>The SARS-CoV-2 non-structural protein 13 (NSP13) has been identified as a target for anti-viral therapeutics due to its highly conserved sequence and is essential for viral replication.  NSP13 is part of the helicase superfamily 1B. As an NTPase and RNA helicase, NSP13 binds to RNA-dependent RNA polymerase and acts in concert with the replication-transcription complex to stimulate backtracking and further activate NSP13 helicase activity. These factors make NSP13 a good target for developing new antiviral drugs. In addition, the identification of epitopes within the NSP13 sequence can help design more effective SARS-CoV-2 vaccines.Models have predicted epitopes exhibiting antigenicity, stability and interactions with MHC class-I and class-II molecules. NSP13 (581-595) is an epitope candidate with various HLA restrictions. This epitope can be used to better vaccine design for more durable CD4+ and CD8+ T cell responses for long-lasting immunity.</p>

<p>Cyclic L27-11 is a peptidomimetic compound that targets the outer membrane protein LptD. LptD is part of a complex that functions to transport lipopolysaccharides to the cell surface through the C-terminal β-barrel domain lumen, embedded within the outer membrane. Cyclic L27-11 can bind LptD and prevent translocation of lipopolysaccharides across the periplasm, even nanomolar doses were effective. This interaction gives cyclic L27-11 a potent antimicrobial activity particularly against the human pathogen Pseudomonas aeruginosa.Modifications of cyclic L27-11 are under research to improve its stability for drug delivery. The peptide provided here has a D-proline substitution, characterised as have no antimicrobial activity against Pseudomonas aeruginosa.</p>

<p>AZD 7140 is a distal radius fracture treatment device that is designed to deliver a controlled force to the distal radius and to provide fluid therapy. It is made of an elastomeric material that has been shown to both access and distribute fluids in the proximal region of the forearm. The device has been shown to provide a force of up to 375 N, which can be delivered in three modes: constant, intermittent, or pulsatile. This device has also been shown to have no adverse effects on bone healing or blood flow.</p>

<p>Portion of Influenza A</p>

<p>TP10 is an amphipathic cell-penetrating peptide (CPPs) known as transportan 10. Its formation involves the use of a lysine residue to form a chimeric linkage between a mastoparan 21-residue peptide, a wasp venon 14-residue peptide and 6-residues derived from the neuropeptide galanin. Structurally TP10 contains only positively charged amino acids along with 4 lysines and an N-terminus. Therefore it will produce a +5 charge under conditions of a neutral pH. It has been found that TP10 may aid molecules in penetrating through the cell membrane barrier through directly interacting with the lipid bilayer. During these interactions with the membrane TP10 will form an amphipathic α-helix. Consequently TP10 can be used in transduction methods.It contains 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag.</p>

<p>Histone H3 (1-20)-GG-[Lys(5-FAM)] is derived from Histone 3 (H3) which is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Both H4 and H3 are highly conserved and perform roles in binding to segments of DNA which enter and leave the nucleosome and in chromatin formation. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, 'histone tail' which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. Due to histones containing a large number of lysine and arginine residues they have a positive net charge which interacts in an electrostatic manner with the negatively charged phosphate groups in DNA. The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter the positioning of the nucleosome, allowing the DNA it to be either available or inaccessible to the transcription machinery.Histone H3 (1-20)-GG-[Lys(5-FAM)] has a C-terminal GKK linker labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag. This peptide also has an uncharged C-terminal amide.</p>

<p>Peptide Ac-CAF-NH2 is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>Peptide H-FNKPFVFLMIEQNTK^-OH is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>3xFlag repeats [DYKDDDDK], supplied as the ammonium salt. An epitope for capture and detection containing an enterokinase site [DDDDK].</p>

<p>Residues 38-54 of neurofilament light polypeptide (NEFL), a neuronal intermediate filament (IF) which forms the major cytoskeletal structure of the axon. Neurofilaments help maintain the morphology of neurons, regenerate myelinated axons, and help transport neurotransmitters to axons and dendrites.The NEFL gene is located in the chromosomal region 8p21, which is a genetic locus frequently found to have deletions in a variety of common human cancers. Perturbations in NEFL have been linked to a number of cancers including breast cancer and head and neck cancer and NEFL-has been shown to act as a tumour suppressor. In addition to its role in cancers, mutations in NEFL have also been linked to motor neuron diseases, such as Charcot-Marie-Tooth disease (CMT).</p>

<p>HIV1 p24 antibody was raised in mouse using HIV1 p24 (native antigen) as the immunogen.</p>

<p>Custom research peptide; min purity 95%. For different specs please use the Peptide Quote Tool</p>

<p>Rabbit polyclonal ALPL antibody (biotin)</p>

<p>Farnesyl pyrophosphate synthase inhibitor</p>

<p>Complement C3 is a fundamental factor featured in all three complement system pathways: the classical, lectin and alternative. To activate the complement cascade C3 associates with C3 convertase to produce C3a and C3b. It is also thought that C3 can be cleaved by proteases outside of the complement cascade. C3b can bind to carbohydrate and protein hydroxyl groups through a thioester bond generated by C3 convertase cleavage. This action allows C3b to be used as a 'marker of foreign molecules such as pathogens and ultimately leads to the assembly of the membrane attack complex (MAC) and anaphylatoxin production.Overall the main functions of the complement system are to mark cells for phagocytosis, the recruitment of inflammatory cells and the cell lysis of bacteria cell by the MAC. The anaphylatoxins C3a and C5a recruit neutrophils and causes the inflammatory response while the MAC produces pores in the bacterial membrane thus causing a Ca2+ influx into the cell and bacterial cell death.The complement system as a whole can be associated with the neurological diseases, bacterial meningitis, thrombotic disorders, neurological and autoimmune diseases.The Leucine residue at position 16 of Complement C3 has been isotopically labelled with carbon-13 (6) and nitrogen-15 (1).</p>

<p>The development of type I diabetes occurs when auto-aggressive T cells are not controlled by T regulatory cells and are allowed to destroy pancreatic islet cells. This ultimately eliminates the production of insulin within the body.  A more precise understanding of the regulatory T cell's antigen specificity by using antigens can provide significant clinical insights into the development of long-term diabetes. The proinsulin 90-104 fragment was used to test antigen-specific T-cell responses in type I diabetes CD4+ T cells. This peptide has helped show that the development of diabetes is linked to an increase in proinsulin-specific T regulatory cells, which may be regulating the rise in auto-aggressive T cells. Further work with the proinsulin 90-104 peptide could help design novel antigen-specific therapy.</p>

<p>Peptide H-DI^TP^TLTL^YVGK^-OH is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>Peptide Ac-QVPLRPMTYK-OH is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>Potent and selective GSK3-β inhibitor, with IC50 value of 48 nM. Increases Nanog expression and regulates self-renewal in murine embryonic stem cells (ESCs). Induces reprograming of induced pluripotent stem cells (iPSCs) to derive cells from specific lineages.</p>

<p>Integral membrane protein TGN38 cycles via endosomes between the cell surface and the trans-Golgi network (TGN). Peptides conjugated to the TGN38 sequence inhibit vesicle budding from TGN membranes-in vitro.-This leads to the conclusion that TGN38 is crucial for forming constitutive exocytic vesicles. The trafficking motif SDYQRL in the cytoplasmic domain at the C-terminal contains a critical tyrosine residue (Y). The motif interacts with the medium-chain adaptor subunits of AP-1/2 to allow sorting from the TGN. Mutation of the key tyrosine dramatically reduces binding affinity to AP-1/2. The flanking sequences to the trafficking motif affect the TGN38 localisation of hybrid proteins. Working with the mouse allele TGN38a has helped understand the conservation between mammalian, rat, and mouse TGN protein sequence and function. The TGN38a trafficking motif with flanking regions is provided here in an acid form with N-terminal acetylation.</p>

<p>This product is part of our Bio-X ™ Range. These products are aimed at life science researchers who need high quality ready-to-use products for assay development, screening or other R&amp;D work. With a solubility datasheet and convenient vials, all of our Bio-X ™ products are in stock across our global warehouses for rapid delivery and ease of use.</p>

<p>The SARS-CoV-2 spike protein is present on the outside of the virus particles and can bind to angiotensin-converting enzyme II (ACE2) present on the host cells. The C-terminal receptor binding domain (RBD) of the spike protein binds to the N-terminal peptidase M2 domain of ACE2. This receptor binding results in the internalisation of the virus-receptor complex and is, therefore the mechanism of entry of SARS-CoV-2 into host cells.The spike protein residues GRLQSLQTY (999-1007) from C have been identified as a T-cell epitope with a predicted HLA restriction. Immune targeting of confirmed epitopes may potentially offer protection against SARS-CoV-2 and help the development of vaccines for long-lasting immunity.</p>

<p>CA 125 antibody was raised in mouse using purified human ovarian mucin antigen from ascitic fluid as the immunogen.</p>

<p>Segment 18-39 of adrenocorticotropic hormone (ACTH). ACTH, also known as corticotropin, is a cleavage product from a larger precursor proopiomelanocortin (POMC). This 39 amino acid-peptide hormone is produced in the anterior pituitary gland upon stimulation by the corticotropin releasing hormone from the hypothalamus in response to stress. It stimulates the secretion of steroid hormone, specifically glucocorticoids in the adrenal cortex by acting through a cell membrane receptor (ACTH-R). In mammals, the action of ACTH is limited to those areas of the adrenal cortex in which the glucocorticoid hormones cortisol (hydrocortisone) and corticosterone are formed. ACTH has little control over the secretion of aldosterone, the other major steroid hormone from the adrenal cortex.</p>

<p>This peptide is recognised and cleaved by the enzyme Sortase A (SrtA) from-Staphylococcus aureus. The catalytic cysteine residue in the active site of SrtA serves as a nucleophile to cleave the peptide bond between threonine and glycine. Cleavage results in the formation of a thioacyl intermediate between the peptide and SrtA. This intermediate is then resolved by the N-terminus of an (oligo)glycine nucleophile, resulting in the creation of a new peptide bond that links the peptide and its fluorescent dye to the incoming nucleophile. This method of protein labelling is known as sortagging.5-Carboxytetramethylrhodamine (5-TAMRA) is a widely used fluorescent dye which excites at 546 nm and emits at 579 nm.</p>

<p>Peptide Ac-TASSYFTNMFATWSPSKARL-NH2 is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.</p>

<p>Streptavidin antibody was raised in mouse using the streptavidin molecule of Streptomyces avidinii as the immunogen.</p>

<p>Discs large homolog 4 (DLG4), also known as postsynaptic density protein 95 (PSD-95), is involved in glutamate trafficking and targeting in synapses. Changes in its expression are associated with neurological disorders. The arginine residue at position 15 is isotopically labelled with carbon-13 (6) and nitrogen-15 (4).</p>

<p>Apolipoprotein B-100 (apoB-100) is a highly expressed human protein, which is targeted by T- and B-cell immune responses in a subgroup of Lyme arthritis (LA) patients. ApoB-100 is present at high levels in the joint fluid of these patients. The high levels of apoB-100 in joints may lead to antigen presentation of apoB-100 peptides by human leukocyte antigen (HLA)-DR molecules, and to T- and B-cell responses to the protein making this protein a novel autoantigen. HLA-DR-presented peptides of apoB-100 have been shown to be immunoreactive.Lyme arthritis (LA) is a late manifestation of infection with the tick-borne spirochete,-Borrelia burgdorferi.The leucine residue at position three of this peptide is isotopically labelled with carbon-13 (6) and nitrogen-15 (1), giving this peptide a mass increase of 7 compared to the unlabelled peptide.</p>

<p>H-LNDPLCLVHPSS-OH is a custom peptide that can be synthesized rapidly by our peptide experts in under 2 weeks. H-LNDPLCLVHPSS-OH is provided at greater that &gt;98% purity (HPLC) from Cymit Quimica for a variety of research applications. We also offer H-LNDPLCLVHPSS-OH in bulk quantities in addition to our standard pack sizes.Please enquire for more information about H-LNDPLCLVHPSS-OH at the technical inquiry form on this page</p>

<p>Albumin (50-62) Bovine is derived from the globular protein Albumin and is found in the blood plasma of humans (known as Human Serum Albumin, HSA) where it serves to maintain plasma pressure and nutritional balance. Another role it carries out is the transportation of bound molecules through the blood. Bovine serum albumin (BSA), composed of 583 amino acids, is very similar to HSA thus allowing BSA to be used as a successful model and a standard protein in laboratory experiments.Although BSA and HAS share homology in their three domains, I, II and III, BSA contains 2 tryptophan whereas HAS only contains 1 tryptophan residue. In agriculture the presence of the albumin protein has been used to assess the health of cows to ensure that a suitable quality of milk and meat are produced. Moreover it is important to detect bovine albumin in food and pharmaceutical products due to it being an allergenic protein.In agriculture the presence of the albumin protein has been used to assess the health of cows to ensure that a suitable quality of milk and meat are produced. Moreover it is important to detect bovine albumin in food and pharmaceutical products due to it being an allergenic protein.</p>

<p>EBV BZLF1 (40-48) (HLA-E) is an immunodominant CEF control peptide that is derived from the Epstein-Barr virus (EBV). EBV targets B cells, which can cause lytic infection and the consequent death of these cells. Natural killer (NK) cells, invariant (iNKT) cells, CD4T cells and CD8 T cells are essential to control the action of EBV-infected cells. EBV BZLF1 (40-48) (HLA-E) is defined as a CEF control peptide due to its antigenic properties. Clinically, these peptides are suitable epitopes for CD8+ T cells and can be used to stimulate the release of IFNg. HLA-E refers to the cell HLA type that this peptide acts on.The BZLF1 protein mediates the transition from the latent stage to the lytic stage of the virus's infection. The lytic stage of the γ herpesvirus has been found to be associated with human carcinogenesis and BZLF1's ability to activate p53 may allow it to induce DNA damage. Structurally it is similar to the leucine zipper family of transcriptional factors.</p>