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Aldol® 515 L-proline amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-proline aminopeptidase activity. The colorless enzyme substrate is cleaved by L-proline aminopeptidase produced by microorganisms grown in liquid or solid media, yielding red-orange color. In addition, red fluorescence signals are generated when a fluorescence enhancer or a suitable matrix is present. Aldol® 515 L-proline amide is suitable for use under aerobic and anaerobic conditions.

5-Bromo-6-chloro-1H-indol-3-yl-a-D-mannopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in lysosomal storage disorder screening.

N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

2,4-Dinitrophenyl)-L-lysine hydrochloride monohydrate is a lysine derivative that has been modified with a 2,4-dinitrophenyl group. It could be used as an enzyme substrate to monitor enzymes that recognize and modify lysine residues, such as lysine methyltransferases and demethylases, lysine acylases and lysine oxidases.

Used in biological studies as a chromogenic substrate for β-galactosidase; red color.Chlorophenol red indicates changes of pH in the range of 4.8 to 6.7 by turning the solution from yellow (at pH 4.8) to violet (at pH 6.7). Converted as signalogenic moiety of an enzyme substrate, chlorophenol red beta-D-galactopyranoside has a good proven sensitivity in the detection of beta-galactosidase activity in transfected mammalian cells, when compared to ONPG (o-nitrophenyl-beta-D-galactopyranoside) (Eustice, 1991)

6-Benzoyl-2-naphthylphosphate disodium salt is a chromogenic substrate used to detect and quantify alkaline phosphatase activity. After enzymatic hydrolysis, 6-benzoyl-2-naphthol is released, which can be detect or even further react with other reagents (e.g. Fast Red) to increase the intensity of the colorimetric signal. It is used in common applications such as ELISA, immunohistochemistry and Western Blotting.

Remazol Brilliant Blue R D-xylan is a chromogenic substrate used for the detection and quantification of xylanase enzyme activity. This substrate consists of a xylan polymer backbone covalently linked to the Remazol Brilliant Blue R dye. Upon cleavage by xylanase enzymes, the dye is released, producing a vibrant blue color that can be easily measured spectrophotometrically. This substrate is widely used in various applications, including the study of xylanase-producing microorganisms, optimization of enzyme production, and evaluation of enzyme kinetics.

Methyl 4-nitrophenyl hexylphosphonate is a phosphonate inhibitor of the enzyme serine esterase. It has been shown to be more potent than other irreversible inhibitors such as ethyl 4-nitrophenyl hexylphosphonate and methyl 4-nitrophenoxybutyl phosphate. This compound is an immobilized form of the methyl 4-nitrophenyl hexylphosphonate that is immobilized on an inert surface, such as silica gel, via a covalent bonding process. The immobilization of the molecule ensures that it will not leach from the surface and is stable over time. The molecular weight of this substance is 411.5 Daltons and its molecular formula is C8H15NO4P. This substance has a diameter of 0.8 nm and can be found in crystalline form with a melting point of 143 degrees Celsius at atmospheric pressure. Methyl 4-nitrophenyl