Enzymes

<p>Isoschizomers: HindII<br> Source: Haemophilus influenzae Rc<br> 5'-GTY↓RAC-3'<br> 3'-CAR↑YTG-5 </p>

MspCI is an endonuclease enzyme isolated from strains of Micrococcus species and is part of the restriction modification system. It is an isoschizomer of AflII. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CTTAAG, which, as the complementary sequence is GAATTC, has rotational symmetry.

Isoschizomers: AhlI, BcuI Sphaerotilus species A↓CTAG T-3' 3'-T GATC↑A-5'

<p>PstI is an endonuclease enzyme isolated from strains of Providencia stuartii, recombinant, E.coli and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is CTGCAG, which, as the complementary sequence is GACGTC, has rotational symmetry. </p>

<p>Isoschizomers: FauNDI<br> Source: Neisseria denitrificans<br> 5'-CA↓TA TG-3'<br> 3'-GT AT↑AC-5 </p>

<p>BioLit QuickRxn EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa. In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry. The reaction is conducted for 5 -15 minutes at 37°C ( Quick digestion with high fidelity) and heat-inactivated at 65°C for 20 minutes. Not sensitive to dam, dcm but Inhibition by CpG methylation. It is accompanied with the specific QuickRxn Buffer to enable 100 % enzyme activity.Concentration : 20U/mcl </p>

<p>BglI is an endonuclease enzyme isolated From strains of Bacillus globigii, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme.The enzyme recognizes internal nucleotides of the SfilI recognition site. It cuts similarly.</p>

<p>PvuII is an endonuclease enzyme isolated from strains of Proteus vulgaris, recombinant, E. coli, and is part of the restriction modification system.In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is CAGCTG which, as the complementary sequence is GTCGAC, has rotational symmetry.</p>

NcoI is an endonuclease enzyme isolated from strains of Nocardia corallina and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is CCATGG which, as the complementary sequence is GGTACC, has rotational symmetry.

<p>HindIII is an endonuclease enzyme isolated from strains of Haemophilus influenzae Rd, and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is AAGCTT, which, as the complementary sequence is TTCGAA, has rotational symmetry. </p>

DraI is an endonuclease enzyme isolated from strains of Deinococcus radiophilus having the DraI gene, and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme.It recognizes the following sequence and produces a blunt end.

<p>Isoschizomers: BfuCI, Bsp143I, BstENII, BstMBI, DpnII, Kzo9II, NdeII<br> Neoschizomers: BstKTI<br> Source: Streptomyces species<br> 5'-↓GATC -3'<br> 3'- CTAG↑-5' </p>

<p>HpaI is an endonuclease enzyme isolated from strains of Haemophilus parainfluenzae, recombinant, E. coli and is part of the restriction modification system.<br> In molecular biology it is used as a restriction enzyme. It creates blunt ends. The nucleic acid sequence where the enzyme cuts is GTTACC, which, as the complementary sequence is CAATGG, has rotational symmetry. </p>

<p>Source: Sphaerotilus species<br> 5'-AAT↓ATT-3'<br> 3'-TTA↑TAA-5' </p>