Enzyme Substrates

4-Trifluoromethylumbelliferyl β-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase . Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl β-D-galactopyranoside is commonly used in bacterial detection and in LacZ gene expression assays.

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Boc-Val-Pro-Arg-PAB-Resorufin is fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex.

5-Bromo-4-chloro-3-indoxyl-1,3-diacetate (BCID) is a potent and selective inhibitor of the tyrosine kinase enzyme. BCID has been shown to inhibit the growth of various cell cultures in vitro including murine and human cells. BCID has been shown to be an effective inhibitor of TNF-α production in murine models for asthma and allergic rhinitis. The drug has also been shown to reversibly inhibit the activity of the enzyme without affecting its ability to bind substrate or to regenerate active enzyme form inactive enzyme, indicating that it does not act as a suicide inhibitor.

Luc-Enterococcus K is a conjugate of the bacterial luciferase enzyme and Enterococcus faecalis. It is used in food testing, environmental testing and diagnostics. Luc-Enterococcus K can be used as a replacement for the fluorogenic substrate luminol, which is difficult to handle because it has to be kept cold during use and is explosive. Luc-Enterococcus K produces light when it reacts with the chromogenic substrate luciferin, which can be stored at room temperature without degrading. The light produced by Luc-Enterococcus K is blue green in color, making it easy to detect with a camera or eye.

4-Methylumbelliferyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate for β-glucuronidase. It is used in diagnostic applications and as a substrate in enzyme kinetics experiments. 4-Methylumbelliferyl-beta-D-glucuronic acid methyl ester has fluorescence properties that allow it to be detected by chemiluminescence and bioluminescence.

Chromogenic substrate for α-D-Fucosidase yielding a blue precipitate.

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Ready-to-use chemiluminescence substrate solution for AP detection

Crimson-Gal (Crimson-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a crimson colored precipitate. Crimson-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.

4-Trifluoromethylumbelliferyl b-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucopyranoside is commonly used in bacterial detection and in Gaucher disease diagnosis.

Resorufin b-D-glucopyranoside is a stable red fluorogenic galactosidase substrate.

5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.

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Chromogenic substrate targeting beta- galactosidase and whose color is dependent on the metal chelating.Alizarin-2-beta-D-galactopyranoside is a chromogenic substrate for enzymes based on alizarin and has been reported for the detection of bacterial beta-galactosidase by researchers at the University of Northumbria and the Freeman Hospital, Newcastle upon Tyne in the UK (James, 2000).

Luciferin is a product that will react with oxygen in the presence of luciferase BX180145 to release energy in the form of light.  UV wavelength - 383 to 387 nmMolar extinction coefficient - min. 17500 L/(mol cm)

Ac-DEVD-6-amino-D-luciferin is a substrate for luciferase that can be used in diagnostic, bioluminescence, and chemiluminescence assays. Ac-DEVD-6-amino-D-luciferin is a high quality compound that has been conjugated with various ligands. The CAS number for Ac-DEVD-6-amino-D-luciferin is 612061–87–8.

A substrate used for the diagnosis of Sanfilippo disease type D (MPS IIID).

Fluorescent dye substrate for flow cytometric measurements