Enzyme Substrates

<p>The AquaSpark® 510 Nitroreductase/NADH Probe (CL-NTR) is a water-soluble probe for the selective chemiluminescent detection of nitroreductase (NTR) activity. It is a versatile tool for monitoring a number of biological processes.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-α-D-glucopyranoside is a high-quality chromogenic pNP enzyme substrate specifically designed to enable effective and accurate detection of enzyme activity. By utilizing this advanced substrate, researchers and professionals can perform a range of applications, including activity assays, enzyme inhibition studies, and substrate specificity profiling. This substrate's unique properties result in exceptional sensitivity and throughput, ensuring effective results for a wide variety of enzyme assays.</p>

<p>Used for synthesis chiral amines from ketones</p>

<p>Please enquire for more information about 4-Nitrophenyl valerate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid, also known as p-nitrophenyl N-acetylneuraminic acid, is a chromogenic substrate specifically designed for the detection and quantification of neuraminidase (sialidase) activity. Upon cleavage by neuraminidase, it releases a yellow-colored product, 4-nitrophenol, which can be easily monitored spectrophotometrically at 405 nm. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of neuraminidase activity in biological samples such as viruses, bacteria, and cell lysates. It is particularly useful for studying the enzymatic properties of neuraminidases from different sources and for the development of novel antiviral and antibacterial agents targeting neuraminidase activity.</p>

<p>The photorelease of 2-deoxy-2-fluoro-L-fucose from 2-nitrobenzyl-2-deoxy-2-fluoro-L-fucopyranoside was performed under a 365 nm LED illumination. It has been shown in plant cells this liberated 2-deoxy-2-fluoro-L-fucose can be converted by the endogenous metabolic machinery into the corresponding nucleotide-sugar, which then efficiently inhibits Golgi-localized fucosyltransferases.</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-4-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Please enquire for more information about 1,2,4-trihydroxynaphthalene-2-O-b-D-glucopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Methyl 4-nitrophenyl hexylphosphonate is a phosphonate inhibitor of the enzyme serine esterase. It has been shown to be more potent than other irreversible inhibitors such as ethyl 4-nitrophenyl hexylphosphonate and methyl 4-nitrophenoxybutyl phosphate. This compound is an immobilized form of the methyl 4-nitrophenyl hexylphosphonate that is immobilized on an inert surface, such as silica gel, via a covalent bonding process. The immobilization of the molecule ensures that it will not leach from the surface and is stable over time. The molecular weight of this substance is 411.5 Daltons and its molecular formula is C8H15NO4P. This substance has a diameter of 0.8 nm and can be found in crystalline form with a melting point of 143 degrees Celsius at atmospheric pressure. Methyl 4-nitrophenyl</p>

<p>4-Nitrophenyl 2-acetamido-3,6-di-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is a fluorogenic substrate that is used in enzyme assays to detect the presence of an enzyme. The substrate reacts with the enzyme to produce a fluorescent product. 4NPAAG can be used for diagnostics, culture media, food testing and environmental testing.</p>

<p>4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.</p>

<p>Bioluminescent substrate for detection of E. coli.</p>

<p>Please enquire for more information about H-Ala-Phe-Pro-pNA trifluoroacetate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chromogenic substrate targeting alpha-D-galactosidase</p>

<p>Luciferin-beta-D-glucuronide, dipotassium salt is a fluorescent compound that is used as a diagnostic tool. It can be used to detect the presence of enzymes, such as beta-galactosidase and beta-glucuronidase, in culture media or biological fluids. Luciferin-beta-D-glucuronide, dipotassium salt is also used for food testing and environmental testing.</p>

<p>6-Hexanoylamino-4-methylumbelliferyl b-D-galactopyranoside is a chromogenic substrate for beta-galactosidase.</p>

<p>4-Methylumbelliferyl beta-D-xylobioside is a fluorogenic substrate for xylanase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-xylobiosideas a substrate for measuring the xylanase activity is used for detecting bacterial infection and gut microbiome analysis.</p>

<p>Please enquire for more information about 1,8,15,22,29,36-Hexaazacyclodotetracontane-2,7,16,21,30,35-hexone including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>6,8-Difluoro-4-methylumbelliferyl b-D-galactopyranoside, commonly known as DiFMUG, is a fluorogenic substrate to detect beta-galactosidase. It has higher sensitivity than the common MUG (4-methylumbelliferyl beta-D-galactopyranoside) subtrate.</p>

<p>Please enquire for more information about cis-6,7-Dimethyl-5,6,7,8-tetrahydropterin hydrochloride including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Chloro-1-naphthol is a chemical compound that has been used in clinical pathology to identify human immunoglobulin. It is also used as a potential biomarker for the diagnosis of Alzheimer's disease. 4-Chloro-1-naphthol binds to the amyloid fibrils and has anti-angiogenic effects in the hippocampus, which may be due to its ability to inhibit the formation of new blood vessels. This chemical compound can be used as a substrate molecule for electrochemical impedance spectroscopy and is used in wastewater treatment.</p>

<p>Chemiluminescence substrate for AP detection</p>

<p>5-Iodo-3-indolyl b-D-galactopyranoside is a chromogenic substrate used for beta-galactosidase. It is incorporated into media or detection kits for rapid and direct identification of microorganisms.</p>

<p>AquaSpark® Enhanced HRP Detection Solution is a sensitive chemiluminescent substrate solution for detecting horseradish peroxidase (HRP) conjugates on immunoblots (Western blot, dot blot) and in enzyme-linked immunosorbent assays (ELISA). The ready-to-use detection solution contains the AquaSpark® substrate and the oxidizing reagent hydrogen peroxide (H2O2).</p>

<p>Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>Fluorogenic substrate for β-glucosidase</p>

<p>Chromogenic substrate for alkaline phosphatase</p>

<p>Fluorogenic substrate for β-galactosidase</p>

<p>4-Nitrophenyl-beta-D-glucopyranoside is a fluorescent probe that can be used in the study of the biochemical properties of enzymes. This compound binds to the enzyme's active site, which allows for transfer reactions to occur. 4-Nitrophenyl-beta-D-glucopyranoside is an artificial substrate that is biochemically inert and has a similar physical structure to natural substrates. It can be used as a model system to study enzymatic reactions and has been shown to have pharmacokinetic properties and a pH optimum. The detection sensitivity of this compound is high, making it ideal for use in biochemical studies.</p>

<p>4-Methylumbelliferyl beta;-D-glucuronide (MUG) is commonly used as a substrate for detecting beta;-glucuronidase. MUG is used for identifying E. coli contamination in food, drinkingwater, clinical samples and for rapid bacterial identification in blood cultures. It is recommended in international normatives (ISO, DIN) as a fluorogenic substrate for detection of E. coli. MUG is also a tool assaying the glucuronidase gene in plants.</p>

<p>5-Amino-3-indoxyl-b-D-galactopyranoside is a chromogenic substrate for the enzyme galactosidase. 5-Amino-3-indoxyl-b-D-galactopyranoside is used in diagnostic tests to identify the presence of lactose intolerance. It can also be used to detect traces of blood in food, water, and other substances. This product has a high purity that makes it suitable for conjugation applications.</p>

<p>Chromogenic substrate targeting cellulases</p>

<p>Chemiluminescent phosphatase substrate in a wide pH range (pH 6 - 10)</p>

<p>A substrate for visualising alkaline phosphatase activity. Produces a red colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.</p>

<p>Chemiluminescence substrate for C2-esterase (acetyl esterase)</p>

<p>Please enquire for more information about 4-Methylumbelliferyl b-D-xylopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>5-Cyano-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that can be used to measure the activity of esterases and other enzymes. It has been shown to be a potent inhibitor of human erythrocyte esterase. The compound is also a potent inducer of bioluminescence in bacteria, which can be used for diagnostic applications. 5-Cyano-3-indolyl-b-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of phosphatases and other enzymes. This product has an enzyme substrate function, which can be used for enzyme assays. 5CIGP is useful for measuring high levels of enzyme activity in the presence of strong inhibitors such as phenylmethylsulfonyl fluoride (PMSF).</p>

<p>Please enquire for more information about AquaSpark® beta-D-glucoside, 10 mM in DMSO, Ramot at Tel-Aviv University Ltd. Patent family WO 2017/130191 including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Crimson-Gal (Crimson-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a crimson colored precipitate. Crimson-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>Aldol® 518 alpha-D-glucopyranoside is a fluorogenic enzyme substrate used for detection of alpha-D-glucosidase activity. The colorless enzyme substrate is cleaved by alpha-D-glucosidase, generating red fluorescent signal and in some conditions, an orange colour in the visible spectrum can get formed, staining bacterial colonies in faint orange coloration. Aldol® 518 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>5-Bromo-6-chloro-3-indolyl b-D-glucuronide cyclohexylammonium salt is a magenta enzyme substrate commonly used in biochemical assays. This compound is a derivative of indole, containing bromine and chlorine atoms at positions 5 and 6, respectively. The glucuronide moiety is attached to the indolyl ring, and the cyclohexylammonium cation serves as a counterion to balance the charge. This substrate is often utilized in the detection and quantification of β-glucuronidase activity, an enzyme involved in the metabolism of glucuronides. Upon cleavage by β-glucuronidase, the substrate produces a magenta-colored product, allowing for easy visualization and measurement of enzyme activity.</p>

<p>4-Methylumbelliferyl-β-D-xylopyranoside is a fluorescent probe that has been used to visualize the enzyme activities of glycosidases, such as endoglycosidase H and α-amylase. It is also used as a tool in structural analysis of oligosaccharides. 4-Methylumbelliferyl-β-D-xylopyranoside has been shown to inhibit collagenase activity in mice, which may be due to its ability to inhibit protein synthesis and cell division.</p>

<p>Aldol® 470 phosphate, disodium salt is a fluorogenic substrate that is used in the detection of enzyme activity. It can be used to detect the presence of an enzyme by measuring the light emitted from the substrate after it has been converted to fluorescent product. Aldol® 470 phosphate, disodium salt is a chromogenic substrate that is used for detecting and measuring enzyme activity. The product of this reaction can be measured by absorbance or fluorescence spectroscopy, making it useful for many different types of diagnostic tests. This product can be conjugated with a variety of ligands for use in diagnostic assays and research. Aldol® 470 phosphate, disodium salt can also be used in culture media as a bioluminescent substrate for bacterial growth and metabolism studies. This product can also be used as an environmental testing reagent or as a staining agent in microscopy applications.</p>

<p>Bronze-Gal (Bronze-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a bronze colored precipitate. Bronze-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>4-Methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is a chromogenic substrate that is used in the detection of glycosidases. It reacts with enzymes such as alpha-amylase, beta-galactosidase, and beta-glucosidase to produce a fluorescent product. This product can be detected using a fluorometer or spectrophotometer. The CAS number for 4-methylumbelliferyl alpha-L-idopyranosiduronic acid 2-sulfate disodium salt is 1045020-74-3.</p>

<p>Chemiluminescent phosphatase substrate in a wide pH range (pH 6 - 10)</p>

<p>Please enquire for more information about 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-L-fucopyranoside is a chromogenic substrate for alpha-L-Fucosidase. It produces a green-blue color.</p>

<p>Ready-to-use chemiluminescence substrate solution for AP detection</p>