Enzyme Substrates

4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production.

Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.

Fluorogenic substrate for beta-glucuronidase GUS

Fluorescein-dipalmitoylphosphatidylethanolamine triethylammonium salt is a fluorescent phospholipid derivative for phospholipase. This substrate consists of a fluorescein conjugated to DPPE (dipalmitoylphosphatidylethanolamine), a key component of biological membranes. This compound is frequently used in membrane dynamics studies and lipid metabolism research. The fluorescein moiety allows visualization of lipid interactions in model membranes, liposomes, or live-cell imaging applications. The triethylammonium salt form enhances solubility and stability, property that facilitates its use in aqueous and lipid-based environments.

2-Chloro-4-nitrophenyl a-D-maltotrioside (2CNP) is a potent hypoglycemic agent that has been shown to decrease postprandial blood glucose levels in humans. 2CNP is a white crystalline solid that is soluble in water and ethanol. The transfer reactions of 2CNP are enhanced by benzalkonium chloride, which forms an organic complex with the drug. The optimum concentration for the hypoglycemic effect of 2CNP is determined to be 10 μM, which can be detected using an optical sensor. This compound also inhibits α-amylase and other enzymes involved in carbohydrate metabolism, leading to the accumulation of glycogen and lowering the blood glucose level.

5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is a chromogenic substrate used to detect the specific enzymatic activity of alkaline phosphatase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is used in ELISA and molecular biology assays such as lacZ reporter systems.

Luc-Salmonella is a conjugate of the enzyme luciferase and the substrate 5-bromo-4-chloro-3-indolyl phosphate. Luc-Salmonella has been used for diagnostic purposes in the detection of bacteria in culture media, as well as in environmental and food testing. Luc-Salmonella can be used to detect Salmonella, which is a bacterium that can cause gastrointestinal disease. Luminescence is measured using a luminometer or fluorimeter with an excitation wavelength of 485 nm and emission wavelengths at 510 nm. Luminescence from Luc-Salmonella is typically measured after 1 hour incubation with bacterial cells.
Luc-Salmonella has been shown to have high purity and quality due to its unique composition of enzyme substrate. This product has been tested for contamination by sensitive assays such as chemiluminescence, bioluminescence, or fluorescence. The purity level of Luc-Sal

Caspases are a family of protease enzymes playing essential roles in programmed cell death and inflammation. The acronym caspase stands for Cysteine Aspartic Acid Specific Protease due to their specific cleavage of an aspartic acid residue, by mean of a cysteine in the catalytic site of the enzyme._x000D_About 11 or 12 different caspases are known in humans and 10 in mice, participating in numerous different cellular functions. Caspase deficiency, for example, has been identified as a cause of tumor development, while over-activation of some caspases such as caspase-3 can lead to excessive apoptosis that can be observed in several neurodegenerative diseases. Caspases cleave proteins at specific peptide recognition sequences. Ac-DEVD-6-Amino-D-Luciferin (Ac-Asp-Glu-Val-Asp-6-Amino-D-Luciferin, Cat.No_x000D_L-8282) is a synthetic chemiluminescent peptide for the determination of caspase-3 and caspase-7activity.

Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.