Chromogenic Substrates

2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.

2-Nitrophenyl butyrate is a chromogenic enzyme substrate that is commonly used in enzymatic assays to detect lipases and esterases. When hydrolyzed by these enzymes, the substrate yields a yellow product that can be detected spectrophotometrically at 405 nm. The advantage of using 2-nitrophenyl butyrate is its high sensitivity and specificity for detecting lipolytic and esterolytic activities. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the determination of esterase activity in biological samples such as serum, plasma, and cell lysates.

6-Chloro-3-indolyl b-D-glucuronide sodium salt is a chromogenic substrate for beta-glucuronidase, a reporter gene used to monitor gene expression by detecting the enzyme's activity. It yields a salmon colored precipitate upon cleavage.

Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color

5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.

6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.

5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is a chromogenic substrate used to detect the specific enzymatic activity of alkaline phosphatase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl phosphate, dilithium salt hydrate is used in ELISA and molecular biology assays such as lacZ reporter systems.

Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for alpha-glucosidase activity (EC 3.2.1.20, alpha-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.

6-Chloro-3-indolyl b-D-cellobioside is a chromogenic substrate that produces a salmon-pink color after enzymatic cleavage. It is used for detecting the activity of beta-D-cellobiosidase in enzyme assays.

8-Hydroxyquinoline-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used for enzymatic detection of peroxidase. The substrate is available in a variety of forms, including powder and liquid. This product has been tested for environmental and bioluminescence activity, as well as food safety. It has also been shown to be a useful ligand in immunoassays.

6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a pink enzyme substrate commonly used in biochemical research and diagnostic applications. This compound is a derivative of indolyl glucopyranoside, which is known for its ability to produce a colored product upon enzymatic hydrolysis. 6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is particularly useful for studying glycosidases, enzymes that cleave glycosidic bonds in complex carbohydrates. Its pink coloration makes it an ideal choice for colorimetric assays, enabling researchers to monitor enzyme activity in real-time and facilitating the development of new diagnostic tools and therapeutic strategies.

6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a chromogenic substrate for N-acetyl-beta-galactosaminidase. It produces a salmon-pink color after enzymatic cleavage.

Chromogenic substrate for esterase with C9 activity yielding a salmon colored precipitate upon cleavage.

Chromogenic substrate used to measure phospholipase C (PLC) activity

Aldol 458 nonanoate is a fluorogenic substrate that can be used in the detection of enzyme activity particularly C8/C9-esterases, lipases activity producing a yellow/green-orange color. Aldol 458 nonanoate reacts with enzymes, such as oxidoreductases and hydrolases, to produce light. This product is a conjugate of aldol 458 nonanoate and an enzyme substrate for the detection of these enzymes. It has been shown to be useful in food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, conjugate, high purity, enzyme substrate for diagnostics and ligand.

Modified naphtol AS substrate used in histochemical and cytochemical staining

Modified naphtol AS substrate used in histochemical and cytochemical staining

Substrate for horseradish peroxidase

Chromogenic substrate for beta-glucuronidase

Phosphatase substrate

4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.

Inorganic phosphate targeted colorimetric probe

N-(3-Sulfopropyl)-3,3',5,5'-tetramethylbenzidine (TMB) sodium salt is a highly sensitive chromogenic substrate used for the detection and quantification of peroxidase enzyme activity. This water-soluble substrate undergoes a color change from colorless to blue upon oxidation by peroxidase enzymes in the presence of hydrogen peroxide. The reaction can be stopped by adding an acid, resulting in a yellow color that can be measured spectrophotometrically. TMB sodium salt is widely used in various applications, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and other diagnostic tests, due to its high sensitivity, low background signal, and excellent stability.

Aldol® 470 acetate is a Fluorogenic substrate that is used in enzymatic reactions to produce a fluorescent product. Aldol® 470 acetate can be used as a ligand or a chromogenic substrate. This product is also used in diagnostic tests, such as conjugates and chemiluminescence, to detect the presence of certain enzymes or microorganisms. It can be used in culture media to measure the amount of certain enzymes, such as phosphatases or proteases, and has been shown to have high purity and quality. This product has CAS No. 1318785-37-3, 1318785-38-4, 1318785-39-5, 1318785-40-6 and 1318785-41-7.

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.

2-Chloro-4-nitrophenyl a-D-maltotrioside (2CNP) is a potent hypoglycemic agent that has been shown to decrease postprandial blood glucose levels in humans. 2CNP is a white crystalline solid that is soluble in water and ethanol. The transfer reactions of 2CNP are enhanced by benzalkonium chloride, which forms an organic complex with the drug. The optimum concentration for the hypoglycemic effect of 2CNP is determined to be 10 μM, which can be detected using an optical sensor. This compound also inhibits α-amylase and other enzymes involved in carbohydrate metabolism, leading to the accumulation of glycogen and lowering the blood glucose level.